Effects of Supplementary Threonine, Canola Oil or Enzyme on Nutrient Digestibility, Performance and Carcass Traits of Growing-finishing Pigs Fed Diets Containing Wheat Distillers Grains with Solubles

  • Thacker, P.A. (Department of Animal Science, University of Saskatchewan)
  • Received : 2009.05.21
  • Accepted : 2009.07.09
  • Published : 2009.12.01


This trial was conducted to determine the effects of various feed additives on nutrient digestibility, performance and carcass traits of growing-finishing pigs fed diets containing wheat distiller' grains with solubles (WDGS). Seventy-two, individually fed pigs (19.7${\pm}$2.6 kg), were assigned to one of six dietary treatments in a 6${\times}$2 (treatment${\times}$sex) factorial design (N = 12). The control diet was based on wheat and soybean meal while the five experimental diets contained 20% WDGS during the growing period and 12% WDGS during the finishing period. One 20% WDGS diet was unsupplemented while the remaining diets were supplemented with either 0.1% threonine, 5% canola oil, 0.2% enzyme (0.1% Endofeed W containing 1,250 units/g of xylanase and 385 units/g of $\beta$-glucanase and 0.1% Vegpro containing 7,700 HUT/g protease and 75 CMC/g cellulase), or a combination of the three additives at the same levels as those fed separately. The digestibility of dry matter, crude protein and energy were all significantly higher in the control diet than the unsupplemented diet containing 20% WDGS. None of the feed additives improved nutrient digestibility. In addition, none of the additives had any significant effect on gain or feed intake during the growing (19.7 to 43.6) or finishing (43.6 to 114.3 kg) periods or overall (19.7 to 114.3 kg). During the growing period, feed conversion was significantly improved for pigs fed the combination of additives compared with the unsupplemented WDGS diet. During the finishing period and overall, feed conversion was significantly improved for pigs fed 5% canola oil alone or in combination with the other additives. None of the supplements had any effect on carcass traits. These results indicate that WDGS can be successfully used as a partial replacement for soybean meal in diets fed to growingfinishing pigs. However, due to its low energy content, there may be some merit in including high energy ingredients such as canola oil when diets containing WDGS are fed.


  1. Cromwell, G. L., K. L. Herkelman and T. S. Stahly. 1993. Physical, chemical and nutritional characteristics of distillers dried grains with solubles for chicks and pigs. J. Anim. Sci. 71:679-686
  2. Kennelly, J. J. and F. X. Aherne. 1980. The effect of fibre in diets formulated to contain different levels of energy and protein on digestibility coefficients in swine. Can. J. Anim. Sci. 60:717-726
  3. Lan, Y., F. O. Opapeju and C. M. Nyachoti. 2008. True ileal protein and amino acid digestibilities in wheat dried distillers's grains with solubles fed to finishing pigs. Anim. Feed Sci. Technol. 140:155-163
  4. Lowrey, R. S., W. G. Pond, J. K. Loosli and J. H. Maner. 1962. Effect of dietary fat level on apparent nutrient digestibility by growing swine. J. Anim. Sci. 21:746-750
  5. Nyachoti, C. M., J. D. House, B. A. Slominski and I. R. Seddon. 2005. Energy and nutrient digestibilities in wheat dried distillers' grains with solubles fed to growing pigs. J. Sci. Food Agric. 85:2581-2586
  6. Thacker, P. A. 2000. Recent advances in the use of enzymes with special reference to $\beta$-glucanases and pentosanases in swine rations. Asian-Aust. J. Anim. Sci. 13 (Special Issue):376-385
  7. Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597
  8. Wahlstrom, R. C., C. S. German and G. W. Libal. 1970. Corn distillers dried grains with solubles in growing-finishing rations. J. Anim. Sci. 30:532-535
  9. Just, A. 1982. The net energy value of crude fat for growth in pigs. Livst. Prod. Sci. 9:501-509
  10. Schneider, B. H. and W. P. Flatt. 1975. The evaluation of feeds through digestibility experiments. University of Georgia Press, Athens, Georgia, p. 423
  11. Fenton, T. W. and M. Fenton. 1979. An improved procedure for the determination of chromic oxide in feed and faeces. Can. J. Anim. Sci. 59:631-634
  12. Ingledew, W. M. 1993. Yeasts for production of fuel alcohol. In: The yeasts. 2nd Edition Vol 5. Yeast Technology. (Ed. A. H. Rose and J. S. Harrison). Academic Press, NY. pp. 245-291
  13. Spiehs, M. J., M. H. Whitney and G. C. Shurson. 2002. Nutrient database for distiller's dried grains with solubles produced from new ethanol plants in Minnesota and South Dakota. J. Anim. Sci. 80:2639-2645
  14. Zasoski, R. J. and R. G. Burau. 1977. A rapid nitric-perchloric acid digestion method for multi-element tissue analysis. Commun. Soil Sci. Plant Anal. 8:425-436
  15. Azain, M. J. 2001. Fat in swine nutrition. In: Swine nutrition (Ed A. J. Lewis and L. L. Southern). CRC Press, Boca Raton, Florida pp. 95-105
  16. Burkitt, D. P., A. R. Walker and N. S. Painter. 1972. Effect of dietary fibre on stools and transit times and its role in the causation of disease. Lancet 2:1408-1412
  17. Stein, H. H. and G. C. Shurson. 2009. The use and application of distillers dried grains with solubles in swine diets. J. Anim. Sci. 87:1292-1303
  18. Pluske, J. R. and M. D. Lindemann. 1998. Maximizing the response in pig and poultry diets containing vegetable proteins by enzyme supplementation. In: Biotechnology in the Feed Industry, Proceedings of Alltech's 14th Annual Symposium (Ed. T. P. Lyons and K. A. Jacques). Nottingham University Press, Nottingham. pp. 375-392
  19. Barrera, M., M. Cervantes, W. C. Sauer, A. B. Araiza, N. Torrentera and M. Cervantes. 2004. Ileal amino acid digestibility and performance of growing pigs fed wheat-based diets supplemented with xylanase. J. Anim. Sci. 82:1997-2003
  20. Ham, G. A., R. A. Stock, T. J. Klopfenstein, E. M. Larson, D. H. Shain and H. E. Hanke. 1994. Wet corn distillers byproducts compared with dried corn distillers grains with solubles as a source of protein and energy for ruminants. J. Anim. Sci. 77:3246-3257
  21. Sauer, W. C., A. Just, H. H. Jorgensen, M. Fekadu and B. O. Eggum. 1980. The influence of diet composition on the apparent digestibility of crude protein and amino acids at the terminal ileum and overall in pigs. Acta Agric. Scand. 30:448-459
  22. Cole, D. J. A. and S. A. Chadd. 1989. Voluntary food intake of growing pigs. In: The voluntary food intake of pigs (Ed. J. M. Forbes, M. A. Varley and T. L. J. Lawrence). British Society of Animal Production Occasional Publication Number 13, Edinburgh, UK. pp. 61-70
  23. Thacker, P. A. 2006. Performance and carcass traits of growingfinishing pigs fed diets containing graded levels of wheat distillers grains with solubles. Can. J. Anim. Sci. 86:527-529
  24. Widyaratne, G. P. and R. T. Zijlstra. 2007. Nutritional value of wheat and corn distillers dried grain with solubles: Digestibility and digestible contents of energy, amino acids and phosphorus, nutrient excretion and growth performance of grower-finisher pigs. Can. J. Anim. Sci. 87:103-114
  25. Gabert, V. M., H. Jorgensen and C. M. Nyachoti. 2001. Bioavailability of amino acids in feedstuffs for swine. In: Swine nutrition (Ed. A. J. Lewis and L. L. Southern). CRC Press, Boca Raton, Florida. pp. 151-186
  26. den Hartog, L. A., M. W. A. Verstegen and J. Husiman. 1989. Amino acid digestibility in pigs as affected by diet composition. In: Absorption and utilization of amino acids. Vol III. (Ed. M. Friedman). CRC Press, Boca Raton, FL. pp. 201-216
  27. Saskatchewan Pork International. 2003. Mitchell's Gourmet Foods, Inc., Hog Settlement Grid. Available online at
  28. Varel, V. H. and W. G. Pond. 1985. Enumeration and activity of cellulolytic bacteria from gestating swine fed various levels of dietary fibre. Appl. Environ. Microbiol. 49:858-862
  29. Bell, J. M., A. Shires and M. O. Keith. 1983. Effect of hull and protein contents of barley on protein and energy digestibility and feeding value for pigs. Can. J. Anim. Sci. 63:201-211
  30. Li, S. and W. C. Sauer. 1994. The effect of dietary fat content on amino acid digestibility in young pigs. J. Anim. Sci. 72:1737-1743
  31. Lindeman, M. D., J. L. Gentry, H. J. Moneque, G. L. Cromwell and K. A. Jacques. 1997. Determination of the contribution of an enzyme combination (Vegpro) to performance in growerfinisher pigs. In: Manipulating pig production VI (Ed. P. D. Cranwell). Australian Pig Science Association, Werribee, Victoria, Australia p. 247
  32. Canadian Council on Animal Care. 1993. Guide to the care and use of experimental animals. Vol. 1. 2nd ed. CCAC, Ottawa, ON. p. 298
  33. Lodge, S. L., R. A. Stock, T. J. Klopfenstein, D. H. Shain and D. W. Herold. 1997. Evaluation of corn and sorghum distillers byproducts. J. Anim. Sci. 75:37-43
  34. Stahly, T. S. 1984. Use of fats in growing pigs. In: Fats in animal nutrition. (Ed. J. Wiseman). Butterworths, Stoneham, MA. pp. 313-331
  35. Shurson, G., M. Spiehs and M. Whitney. 2004. The use of maize distiller's dried grains with solubles in pig diets. Pig News Inform. 25:75N-83N
  36. Widyartne, G. P., J. F. Patience and R. T. Zijlstra. 2009. Effect of xylanase supplementation of diets containing wheat distiller's dried grains with solubles on energy, amino acid and phosphorus digestibility and growth performance of growerfinisher pigs. Can. J. Anim. Sci. 89:91-95
  37. Association of Official Analytical Chemists. 1990. Official Methods of Analysis, 15th edn, AOAC, Washington, DC
  38. Statistical Analysis System Institute, Inc. 1999. SAS/STAT users guide, version 6, 4th Edition. SAS Institute Inc., Cary, NC. p. 515
  39. Myer, R. O. and G. E. Combs. 1991. Fat supplementation of diets containing a high level of oats for growing-finishing swine. J. Anim. Sci. 69:4665-4669
  40. National Research Council. 1998. Nutrient requirements of swine. 10th ed. NAS-NRC, Washington, DC. p. 189
  41. Thacker, P. A. 2001. Effect of enzyme supplementation on the performance of growing-finishing pigs fed barley-based diets supplemented with soybean meal or canola meal. Asian-Aust. J. Anim. Sci. 14:1008-1013

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