DOI QR코드

DOI QR Code

Effects of nanoparticulate saponin-platinum conjugates on 2,4-dinitrofluorobenzene-induced macrophage inflammatory protein-2 gene expression via reactive oxygen species production in RAW 264.7 cells

  • Kim, Young-Jin (Department of Microbiology, College of Medicine, Hallym University) ;
  • Kim, Dong-Bum (Department of Microbiology, College of Medicine, Hallym University) ;
  • Lee, Young-Hee (Department of Biochemistry, College of Natural Sciences, Chungbuk National University) ;
  • Choi, Soo-Young (Department of Biomedical Science, Hallym University) ;
  • Park, Jin-Seu (Department of Biomedical Science, Hallym University) ;
  • Lee, Seung-Yong (BIPL Co., Ltd.) ;
  • Park, Joon-Won (BIPL Co., Ltd.) ;
  • Kwon, Hyung-Joo (Department of Microbiology, College of Medicine, Hallym University)
  • Published : 2009.05.31

Abstract

Nanoparticulate platinum (II) (nano Pt) is a powerful antioxidant that is widely used to scavenge reactive oxygen species (ROS). The antioxidant activity of nano Pt has gained attention as a potentially useful therapeutic for a variety of diseases including cancer and aging. In the present study, we prepared nanoparticulate saponin-Pt (II) (nano saponin-Pt) conjugates using the ethanol reduction method to enhance the permeability and retention effect of Pt. The nano saponin-Pt conjugates were found to restore the viability of approximately 40% of 2,4-dinitrofluorobenzene (DNFB)-treated RAW 264.7 cells. In addition, we found that nano saponin-Pt conjugates acted as a potent antioxidant that reduced the production of ROS and inhibited activation of the MAP kinase pathway and MIP-2 gene expression in response to DNFB. These results provide insight into the potential usefulness of nano saponin-Pt conjugates as a treatment for contact hypersensitivity.

Keywords

DNFB;Macrophages;MIP-2;Nano saponin-Pt;ROS

References

  1. Beckman, K. B. and Ames, B. N. (1997) Oxidative decay of DNA. J. Biol. Chem. 272, 19633-19636 https://doi.org/10.1074/jbc.272.32.19633
  2. Finkel, T. and Holbrook, N. J. (2000) Oxidant, oxidative stress and the biology of ageing. Nature 408, 239-247 https://doi.org/10.1038/35041687
  3. Kitagaki, H., Ono, N., Hayakawa, K., Kitazawa, T., Watanabe, K. and Shiohara, T. (1997) Repeated elicitation of contact hypersensitivity induces a shift in cutaneous cytokine milieu from a T helper cell type 1 to a T helper cell type 2 profile. J. Immunol. 159, 2484-2491
  4. Harada, M. and Einaga, H. (2007) Preparation of Pt/Rh bimetallic colloidal particles in polymer solutions using borohyride- reduction. J. Colloid Interface Sci. 308, 568-572
  5. Cruz, M. T., Duarte, C. B., Goncalo, M., Figueiredo, A., Carvalho, A. P. and Lopes, M. C. (2003) The sensitizer 2,4-dinitrofluorobenzene activates caspase-3 and induces cell death in a skin dendritic cell line. Int. J. Toxicol. 22, 43-48 https://doi.org/10.1080/10915810305069
  6. Yu, J. Y., Jun, Y. J., Jang, S. H., Lee, H. J. and Sohn, Y. S. (2007) Nanoparticulate platinum (II) anticancer drug: synthesis and characterization of amphiphilic cyclotriphosphazene- platinum (II) conjugates. J. Inorg. Biochem. 101, 1931-1936 https://doi.org/10.1016/j.jinorgbio.2007.07.003
  7. Kim D., Kim, J., Kwon, S., Kim, Y. J., Lee, S., Lee, Y., Seo, J. N., Park, C. S., Park, K. L. and Kwon, H. J. (2008) Regulation of macrophage inflammatory protein-2 gene expression in response to 2,4-dinitrofluorobenzene in RAW 264.7 cells. BMB reports 41, 316-321 https://doi.org/10.5483/BMBRep.2008.41.4.316
  8. Kang, K. A., Kang, J. H. and Yang, M. P. (2008) Ginseng total saponin enhances the phagocytic capacity of canine peripheral blood phagocytes in vitro. Am. J. Chin. Med. 36, 329-341 https://doi.org/10.1142/S0192415X08005801
  9. Packer, L. (1992) Interactions among antioxidants in health and disease: vitamin E and its redox cycle. Proc. Soc. Exp. Biol. Med. 200, 271-276 https://doi.org/10.3181/00379727-200-43433
  10. Ahmed, A. R. and Blose, D. A. (1983) Delayed-type hypersensitivity. A review. Arch. Dermatol. 119, 934-945 https://doi.org/10.1001/archderm.119.11.934
  11. Tuschl, H. and Kovac, R. (2001) Langerhans cells and immature dendritic cells as model systems for screening of skin sensitizers. Toxicol. In Vitro 15, 327-331 https://doi.org/10.1016/S0887-2333(01)00030-3
  12. Kajita, M., Hikosaka, K., Iitsuka, M., Kanayama, A., Toshima, N. and Miyamoto, Y. (2007) Platinum nanoparticle is a useful scavenger of superoxide anion and hydrogen peroxide. Free Radic. Res. 41, 615-626 https://doi.org/10.1080/10715760601169679
  13. Herouet, C., Cottin, M., LeClaire, J., Enk, A. and Rousset, F. (2000) Contact sensitizers specifically increase MHC class II expression on murine immature dendritic cells. In Vitro Mol. Toxicol. 13, 113-123 https://doi.org/10.1089/109793300440703
  14. Tomimori, Y., Tanaka, Y., Goto, M. and Fukuda, Y. (2005) Repeated topical challenge with chemical antigen elicits sustained dermatitis in NC/Nga mice in specific-pathogen- free condition. J. Invest. Dermatol. 124, 119-124 https://doi.org/10.1111/j.0022-202X.2004.23516.x
  15. Alley, M.C., Scudiero, D.A., Monks, A., Hursey, M.L., Czerwinski, M.J., Fine, D.L., Abbott, B.J., Mayo, J.G., Shoemaker, R.H. and Boyd, M.R. (1988) Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res. 48, 589-601
  16. Jun, Y. J., Kim, J. I., Jun, M. J. and Sohn, Y. S. (2005) Selective tumor targeting by enhanced permeability and retention effect. Synthesis and antitumor activity of polyphosphazene- platinum (II) conjugates. J. Inorg. Biochem. 99, 1593-1601 https://doi.org/10.1016/j.jinorgbio.2005.04.019
  17. Reedijk, J. (2003) New clues for platinum antitumor chemistry: kinetically controlled metal binding to DNA. Proc. Natl. Acad. Sci. U.S.A. 100, 3611-3616 https://doi.org/10.1073/pnas.0737293100
  18. Cosaert, J. and Quoix, E. (2002) Platinum drugs in the treatment of non-small-cell lung cancer. Br. J. Cancer 87, 825-833 https://doi.org/10.1038/sj.bjc.6600540
  19. Mandal, S., Berube, G., Asselin, E., Richardson, V. J., Church, J. G., Bridson, J., Pham, T. N. Q., Pramanik, S. K. and Mandal, S. K. (2007) A new platinum complex of triazine demonstrates G1 arrest with novel biological profile in human breast cancer cell line, MDA-MB-468. Bioorg. Med. Chem. Lett. 17, 2139-2145 https://doi.org/10.1016/j.bmcl.2007.01.116
  20. Zhang, C. X. and Lippard, S. J. (2003) New metal complexes as potential therapeutics. Curr. Opin. Chem. Biol. 7, 481-489 https://doi.org/10.1016/S1367-5931(03)00081-4
  21. Kim, Y. E., Kang, H. B., Park, J. A., Nam, K. H., Kwon, H. J. and Lee, Y. (2008) Upregulation of NF-κB upon differentiation of mouse embryonic stem cells. BMB Rep. 41, 705-709 https://doi.org/10.5483/BMBRep.2008.41.10.705
  22. Kim, D., Kim, Y. J., Seo, J. N., Kim, J., Lee, Y., Park, C. S., Kim, D. W., Kim, D. S. and Kwon, H. J. (2009) 2,4-Dinitrofluorobenzene modifies cellular proteins and induces macrophage inflammatory protein-2 gene expression via reactive oxygen species production in RAW 264.7 cells. Immunol. Invest. 38, 132-152 https://doi.org/10.1080/08820130802667499
  23. Vital, A. L., Goncalo, M., Cruz, M. T., Figueiredo, A., Duarte, C. B. and Celeste Lopes, M. (2004) The sensitizers nickel sulfate and 2,4-dinitrofluorobenzene increase CD40 and IL-12 receptor expression in a fetal skin dendritic cell line. Biosci. Rep. 24, 191-202 https://doi.org/10.1007/s10540-005-2580-7
  24. Matos, T. J., Duarte, C. B., Goncalo, M. and Lopes, M. C. (2005) Role of oxidative stress in ERK and p38 MAPK activation induced by the chemical sensitizer DNFB in a fetal skin dendritic cell line. Immunol. Cell Biol. 83, 607-614 https://doi.org/10.1111/j.1440-1711.2005.01378.x

Cited by

  1. Pulmonary Effects of Diesel Exhaust vol.179, pp.6, 2011, https://doi.org/10.1016/j.ajpath.2011.08.031
  2. Diesel Exhaust Particulates Exacerbate Asthma-Like Inflammation by Increasing CXC Chemokines vol.179, pp.6, 2011, https://doi.org/10.1016/j.ajpath.2011.08.008
  3. Suberoylanilide hydroxamic acid induces ROS-mediated cleavage of HSP90 in leukemia cells vol.20, pp.1, 2015, https://doi.org/10.1007/s12192-014-0533-4
  4. Cerium oxide and platinum nanoparticles protect cells from oxidant-mediated apoptosis vol.13, pp.10, 2011, https://doi.org/10.1007/s11051-011-0544-3
  5. The Antioxidative Effect of Heat-Shock Protein 70 in Dendritic Cells vol.78, pp.3, 2013, https://doi.org/10.1111/sji.12078
  6. Platinum nanoparticles in nanobiomedicine vol.46, pp.16, 2017, https://doi.org/10.1039/C7CS00152E
  7. Effects of Palladium Nanoparticles on the Cytokine Release from Peripheral Blood Mononuclear Cells of Palladium-Sensitized Women vol.53, pp.9, 2011, https://doi.org/10.1097/JOM.0b013e318228115e
  8. Generation 9 Polyamidoamine Dendrimer Encapsulated Platinum Nanoparticle Mimics Catalase Size, Shape, and Catalytic Activity vol.29, pp.17, 2013, https://doi.org/10.1021/la3046077
  9. Differential expression of cell surface markers in response to 2,4-dinitrofluorobenzene in RAW 264.7 and primary immune cells vol.45, pp.9, 2012, https://doi.org/10.5483/BMBRep.2012.45.9.048
  10. An Early-Stage Atherosclerosis Research Model Based on Microfluidics vol.12, pp.15, 2016, https://doi.org/10.1002/smll.201503241
  11. Proteasome inhibitor-induced cleavage of HSP90 is mediated by ROS generation and caspase 10-activation in human leukemic cells vol.13, 2017, https://doi.org/10.1016/j.redox.2017.07.010