Free Radical Scavenging Activity of Butanol Fraction from the Fruit of Citrus junos

  • Cho, Eun-Ju (Department of Food Science and Nutrition, Pusan National University) ;
  • Li, Li (Department of Food Science and Nutrition, Pusan National University) ;
  • Piao, Xiang-Lan (Chinese Minority Traditional Medical Center, College of Life and Environmental Science, Central University for Nationalities) ;
  • Lee, Sang-Hyun (Department of Applied Plant Science, Chung-Ang University) ;
  • Kim, Hyun-Young (Department of Food Science, Jinju National University)
  • Published : 2009.03.31


In this study, we investigated the free radical [1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (${\cdot}OH$) and superoxide anion (${O_2}^-$)] scavenging activity of MeOH extract and 3 fractions of Citrus junos. Of the tested fractions, the BuOH fraction showed the strongest DPPH scavenging activity, showing the $IC_{50}$ values of 63.4 mg/mL. Therefore, we continuously carried out DPPH, ${\cdot}OH$ and ${O_2}^-$ scavenging activity tests of BuOH fraction of Citrus junos. The BuOH fraction of Citrus junos inhibited DPPH radical to 97.5% at a concentration of 1000 mg/mL and the scavenging activities were increased concentration-dependently. In addition, BuOH fraction from Citrus junos also scavenged ${\cdot}OH$ in a concentration dependent manner from 5 to 1000 mg/mL. Furthermore, BuOH fraction showed about 56% ${O_2}^-$-scavengimg activity at 25 mg/mL concentration but, the scavenging activities were not enhanced in a dose dependent manner. The present results suggest that BuOH fraction of Citrus junos would have the protective potential from oxidative stress induced by free radicals.


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