Isolation of Pentacyclic Triterpenoids from Semi-fermented Tea and Its Effects on Oxidative Stress

  • Chung, Ha-Sook (Department of Foods and Nutrition, College of Natural Sciences, Duksung Women's University)
  • Published : 2009.03.31


Antioxidative activities of major pentacyclic terpenoids from the semi-fermented tea of Camellia sinensis L. were investigated. The free radical scavenging activities of triterpenoids $1{\sim}3$ were examined with of DPPH and superoxide anion radical scavenging activity. The $IC_{50}$ of compounds 1 and 2 for DPPH radical scavenging activities were 23.1 and $37.2{\mu}g/mL$ respectively, and for superoxide anion radical scavenging activities were 37.2 and $35.2{\mu}g/mL$, respectively. According to this result, compounds 1 or 2 in semi-fermented tea could be the candidates for bioactive material having antioxidant activity.


  1. Chung HS, Shin JC. 2007. Characterization of antioxidant alkaloids and phenolic acids from anthocyanin-pigmented rice (Oryza sativa cv. Heugjinjubyeo). Food Chem 104: 1670-1677
  2. Carmody RJ, Cotter TG. 2001. Signaling apoptosis: a radical approach. Redox Rep 6: 77-90
  3. Kim HJ, So YJ, Jang JH, Lee JS, Oh YJ, Surh YJ. 2001. Differential cell death induced by salsolinol with and without copper: possible role of reactive oxygen species. Mol Pharmacol 60: 440-449
  4. Hyun JW, Chung HS. 2006. Medicinal plants with cytotoxic and antioxidative activity. In Recent progress in medicinal plants. Govil JN, Singh VK, eds. Studium Press, Texas, USA. p 193-202
  5. Shin JS, Chung HS. 2007. Antibacterial activities of phenolic components from Camellia sinensis L. on pathogenic microrganisms. J Food Sci Nutr 12: 135-140
  6. Jian L, Lee AH, Binns CW. 2007. Tea and lycopene protect against prostate cancer. Asia Pac J Clin Nutr 16: 453-457
  7. Yen GC, Chen HY. 1996. Relationship between antimutagenic activity and major components of various teas. Mutagenesis 11: 37-41
  8. Woo WS. 2001. Study on Natural Products Chemistry. Seoul National University Press, Seoul, Korea. p 215
  9. Chung HS, Chang LC, Lee SK, Shamon LA, van Breeman RG, Mehta RG, Farnsworth NR, Pezzuto JM, Kinghorn AD. 1997. Flavonoid constituents of Chorizanthe diffusa with potential cancer chemopreventive activity. J Agric Food Chem 47: 36-41
  10. Chung HS. 2000. Cytotoxic triterpenoids on human cancer cell lines from Ixeris sonchifolia. Food Sci Biotechnol 9: 364-367
  11. Budzikiewicz H, Wilson JM, Djerassi C. 1963. Mass spectrometry in structural and stereochemical problems. XXXII. Pentacyclic triterpenes. J Am Chem Soc 85: 3688-3699
  12. Kim JS, Kim YJ, Lee SY, Kang SS. 2008. Phytochemical studies on Paeoniae radix (3)-triterpenoids. Kor J Pharmacogn 39: 37-42
  13. An R-B, Na MK, Min BS, Lee HK, Bae WH. 2008. Anti-complement activity of triterpenoids from the whole plant of Patrinia saniculaefolia. Nat Prod Sci 14: 249-253
  14. Liu J. 1995. Pharmacology of oleanolic and ursolic acid. J Ethnopharmacol 49: 57-68
  15. Shirwaikar A, Setty M, Bommu P. 2004. Effect of lupeol isolated from Crataeva nurvala Buch.-Ham. stem bark extract against free radical induced nephrotoxicity in rats. Indian J Exp Biol 42: 686-690
  16. Sultana S, Saleem M, Sharma S, Khan N. 2003. Lupeol, a triterpene, prevents free radical mediated macromolecular damage and alleviates benzoyl peroxide induced biochemical alterations in murine skin. Indian J Exp Biol 41: 827-831
  17. Hassani AS, Amirmozafari N, Ordouzadeh N, Hamdi K, Nazari R, Ghaemi A. 2008. Volatile components of Camellia sinensis inhibit growth and biofilm formation of oral streptococci in vitro. Pak J Biol Sci 11: 1336-1341
  18. Toyoda-Ono Y, Yoshimura M, Nakai M, Fukui Y, Asami S, Shibata H, Kiso Y, Ikeda I. 2007. Suppression of postprandial hypertriglyceridemia in rats and mice by oolong tea polymerized polyphenols. Biosci Biotechnol Biochem 71: 971-976
  19. Jang HH, Surh YJ. 2003. Protective effects of resveratrol on $\beta$-amyloid induced oxidative PC12 cell death. Free Radic Biol Med 34: 1100-1110
  20. Ueno T, Kiyohara S, Ho CT, Masuda H. 2006. Potent inhibitory effects of black tea theaflavins on off-odor formation from citral. J Agric Food Chem 54: 3055-3061
  21. Ibrahim HR, Hoq MI, Aoki T. 2007. Overtransferrin possesses SOD-like suoeroxide anion scavenging activity that is promoted by copper and manganese binding. Int J Biol Macromol 41: 631-640
  22. Li CS, Black WC, Chan CC, Ford-Hutchinson AW, Gauthier JY, Gordon R, Guay D, Kargman S, Lau CK, Mancini J. 1995. Cyclooxygenase-2 inhibitors. Synthesis and pharmacological activities of 5-methanesulfonamido-1-indanone erivatives. J Med Chem 38: 4897-4905
  23. Saleem M, Maddodi N, Abu Zai M, Khan N, bin Hafeez B, Asim M, Suh Y, Yun JM, Setaluri V, Mukhtar H. 2008. Lupeol inhibits growth of highly aggressive human metastatic melanoma cells in vitro and in vivo by inducing apoptosis. Clin Cancer Res 14: 2119-2127