Antioxidative and Lipofuscin-Formation Inhibitory Effects of Soybean and Chungkukjang

  • Ryu, Bog-Mi (Department of Biohealth Products Research Center, Food Science Institute, and School of Food and Life Science, Inje University) ;
  • Ryu, Seung-Hee (Department of Biohealth Products Research Center, Food Science Institute, and School of Food and Life Science, Inje University) ;
  • Kim, Mi-Jeong (Department of Biohealth Products Research Center, Food Science Institute, and School of Food and Life Science, Inje University) ;
  • Lee, Young-Soon (Department of Food and Nutrition, Kyung Hee University) ;
  • Moon, Gap-Soon (Department of Biohealth Products Research Center, Food Science Institute, and School of Food and Life Science, Inje University)
  • Published : 2009.03.31


To compare antioxidative and antiaging effects between yellow soybean (YS) and Chungkukjang (CK) in vivo system, male Sprague-Dawley rats (n=24) were fed the diets containing YS and CK for 8 weeks, respectively. The YS and CK groups showed the preventive effects on lipid and protein oxidations in liver and plasma. Hepatic SOD and GSH-peroxidase activities were significantly inhibited in CK group. Superoxide anion radicals in cytosol significantly lowered in YS and CK groups compared with control group. In addition, dietary YS and CK effectively inhibited formation of the lipofuscin, the indicator of aging in heart and eye, especially the CK group had a stronger preventive activity in eye. The results of this study showed that YS and CK diet effectively suppressed the superoxide anion radical formation and tissue oxidation.


  1. Messina M. 1995. Modern application for an ancient bean: Soybeans and the prevention and treatment of chronic disease. J Nutr 125: 567-569
  2. Hayes RE, Bookwalter GN, Bagley EBA. 1977. Antioxidant activity of soybean flour and derivatives-A Review. J Food Sci 42: 1527-1532
  3. Pratt DE, Bibrac PM. 1979. Source of antioxidant activity of soybean and soy products. J Food Sci 44: 1720-1722
  4. Yang JH, Mau JL, Ko PT, Huang LC. 2000. Antioxidant properties of fermented soybean broth. Food Chem 71: 249-254
  5. Iwai K, Nakaya N, Kawasaki Y, Matsue H. 2002. Inhibitory effect of natto, a kind of fermented soybeans on LDL oxidation in vitro. J Agric Food Chem 50: 3592-3596
  6. Esaki H, Onozaki H, Osawa H, Osawa T. 1990. Antioxidative activity of natto. Nippon Shokuhin Kohyo Gakkaishi 37: 474-477
  7. Hong JH, Youn HK, Kang MC, Lee HJ, Hur SH. 2000. Properties of bacillus spp. isolated from fermenting Chungkukjang. J Korean Soc Ind Food Technol 4: 67-72
  8. AOAC. 1990. Official Method of Analysis. $15^{th}$ ed. Association of Official Analytical Chemists, Washington, DC, USA
  9. Buege JA, Aust SD. 1978. Microsomal lipid peroxidation. Methods Enzymol 52: 302-306
  10. Oliver CN, Ahn BW, Moerman EJ, Goldstein S, Stadtman ER. 1987. Age-related changes in oxidized proteins. J Biol Chem 262: 5488-5491
  11. Peterson GL. 1977. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem 83: 346-356
  12. Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474
  13. Lawrence RA, Burk F. 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Comm 71: 952-958
  14. Fletcher BL, Dillard CJ, Tappel AL. 1973. Measurement of fluorescent lipid peroxidation products in biological systems and tissues. Anal Biochem 52: 1-9
  15. Baba N, Radwan H, Itallie TH. 1992. Effects of casein versus soyprotein diets on body composition and serum lipid levels in adult rats. Nutr Res 12: 279-288
  16. Nagaoka S, Kanamaru Y, Kuzuya Y, Kojima T, Kuwata T. 1992. Competitive studies on the serum cholesterol lowering action of whey protein and soybean protein in rats. Biosci Biotechnol Biochem 56: 1484-1485
  17. Sarkar PK, Tamang JP. 1995. Changes in the microbial profile and proximate composition during natural and controlled fermentations of soybeans to produce kinema. Food Microbiol 12: 317-325
  18. Kiers JL, Van laeken AEA, Rombouts FM, Nout MJR. 2000. In vitro digestibility of Bacillus fermented soya bean. Int J Food Microbiol 60: 163-169
  19. Martin GM, Austad SN, Johnson TE. 1996. Genetic analysis of ageing: role of oxidative damage and environmental stresses. Nat Genet 13: 25-34
  20. Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stadtman ER. 1990. Determination of carbonyl content in oxidatively modified proteins. Meth Enzymol 186: 464-478
  21. Sohal RS, Brunk UT. 1989. Lipofuscin as an indicator of oxidative stress and aging. Adv Exp Med Biol 266: 17-26
  22. McCord JM, Fridovich I. 1969. Superoxide dismutase. An enzyme function for erythrocuprein. J Biol Chem 24: 6049-6055
  23. Lu LJ, Lin SN, Grady JJ, Nagamani M, Anderson KE. 1996. Altered kinetics and extent of urinary daidzein and genistein excretion in women during chronic soya exposure. Nutr Cancer 26: 289-302
  24. Wang H, Murphy PA. 1994. Isoflavone contnent of commercial soybean foods. J Agric Food Chem 42: 1666-1677
  25. Fukutake M, Takahashi M, Ishida K, Kawamura H, Sugimura T, Wakabayashi K. 1996. Quantification of genistein and genistin in soybeans and soybean products. Food Chem Toxicol 34: 457-461
  26. Hutchins AM, Slavin JL, Lampe JW. 1995. Urinary isoflavonoids phytoestrogen and lignan excretion after consumption of fermented and unfermented soy products. J Am Diet Assoc 95: 545-551
  27. Setchell KDR, Adlercreutz H. 1988. Mammalian lignans and phyto-oestrogens: recent studies on their formation, metabolism and biological role in health and disease. In Role of got flora in toxicity and cancer. Rowland IR, ed. Academic Press Limited, San Diego, CA, USA. p 315-345
  28. Lund T, Munson DJ, Haldy ME, Setchell KDR, Lephart ED, Handa RJ. 2003. Equol has unique potent anti-androgen actions. Proceeding of $5^{th}$ International symposium on the role of soy in preventing and treating chronic disease. Sep 21-24, Orlando, FL, USA. p15
  29. Setchell KDR, Borriello SP, Kirk DN, Axelson M. 1984. Nonsteroidal estrogens of dietary origin: Possible role in hormone-dependent disease. Am J Clin Nutr 40: 569-578
  30. Halliwell B, Gutteridge JMC. 1981. Formation of a thiobarbituric acid reactive substance from deoxyribose in the presence of iron salts. FEBS Letters 128: 347-352
  31. Iwai K, Nakaya N, Kawasaki Y, Matsue H. 2002. Antioxidative functions of natto, a kind of fermented soybeans: Effect on LDL oxidation and lipid metabolism in cholesterol-fed rats. J Agric Food Chem 50: 3597-3601
  32. Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissues by hiobarbituric acid reaction. Anal Biochem 95: 351-358
  33. Aebi H. 1984. Catalase in vitro. Methods Enzymol 105: 121-126
  34. Choi JH, Kim DI, Park SH, Kim DW, Lee JS, Kim HS. 1999. Investigation of anti-aging effect and determination of chemical structures of pine needle extract (PNE) through the animal experiments. 1. Effects of PNE on oxygen radicals and their scavenger enzymes in liver of SD rats. Korean J Life Science 9: 466-472
  35. Madani S, Prost J, Belleville J. 2000. Dietary protein level and origin (casein and highly purified soybean protein) affect hepatic storage, plasma lipid transport, and antioxidative defense status in the rat. Nutrition 16: 368-375
  36. Izumi T, Piskula MK, Osawa S. 2000. Soy isoflavone aglycones are absorbed faster and in higher amounts than their glucosides in humans. J Nutr 130: 1695-1699
  37. Setchell KDR. 2003. Equol-origins, actions and clinical relevance of this specific soy isoflavone metabolite. Proceeding of $5^{th}$ International symposium on the role of soy in preventing and treating chronic disease. Sep 21-24, Orlando, FL, USA. p14

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