DOI QR코드

DOI QR Code

Antioxidative and Lipofuscin-Formation Inhibitory Effects of Soybean and Chungkukjang

  • Ryu, Bog-Mi (Department of Biohealth Products Research Center, Food Science Institute, and School of Food and Life Science, Inje University) ;
  • Ryu, Seung-Hee (Department of Biohealth Products Research Center, Food Science Institute, and School of Food and Life Science, Inje University) ;
  • Kim, Mi-Jeong (Department of Biohealth Products Research Center, Food Science Institute, and School of Food and Life Science, Inje University) ;
  • Lee, Young-Soon (Department of Food and Nutrition, Kyung Hee University) ;
  • Moon, Gap-Soon (Department of Biohealth Products Research Center, Food Science Institute, and School of Food and Life Science, Inje University)
  • Published : 2009.03.31

Abstract

To compare antioxidative and antiaging effects between yellow soybean (YS) and Chungkukjang (CK) in vivo system, male Sprague-Dawley rats (n=24) were fed the diets containing YS and CK for 8 weeks, respectively. The YS and CK groups showed the preventive effects on lipid and protein oxidations in liver and plasma. Hepatic SOD and GSH-peroxidase activities were significantly inhibited in CK group. Superoxide anion radicals in cytosol significantly lowered in YS and CK groups compared with control group. In addition, dietary YS and CK effectively inhibited formation of the lipofuscin, the indicator of aging in heart and eye, especially the CK group had a stronger preventive activity in eye. The results of this study showed that YS and CK diet effectively suppressed the superoxide anion radical formation and tissue oxidation.

References

  1. Messina M. 1995. Modern application for an ancient bean: Soybeans and the prevention and treatment of chronic disease. J Nutr 125: 567-569
  2. Hayes RE, Bookwalter GN, Bagley EBA. 1977. Antioxidant activity of soybean flour and derivatives-A Review. J Food Sci 42: 1527-1532 https://doi.org/10.1111/j.1365-2621.1977.tb08417.x
  3. Pratt DE, Bibrac PM. 1979. Source of antioxidant activity of soybean and soy products. J Food Sci 44: 1720-1722 https://doi.org/10.1111/j.1365-2621.1979.tb09125.x
  4. Yang JH, Mau JL, Ko PT, Huang LC. 2000. Antioxidant properties of fermented soybean broth. Food Chem 71: 249-254 https://doi.org/10.1016/S0308-8146(00)00165-5
  5. Iwai K, Nakaya N, Kawasaki Y, Matsue H. 2002. Inhibitory effect of natto, a kind of fermented soybeans on LDL oxidation in vitro. J Agric Food Chem 50: 3592-3596 https://doi.org/10.1021/jf011718g
  6. Esaki H, Onozaki H, Osawa H, Osawa T. 1990. Antioxidative activity of natto. Nippon Shokuhin Kohyo Gakkaishi 37: 474-477 https://doi.org/10.3136/nskkk1962.37.6_474
  7. Hong JH, Youn HK, Kang MC, Lee HJ, Hur SH. 2000. Properties of bacillus spp. isolated from fermenting Chungkukjang. J Korean Soc Ind Food Technol 4: 67-72
  8. AOAC. 1990. Official Method of Analysis. $15^{th}$ ed. Association of Official Analytical Chemists, Washington, DC, USA
  9. Buege JA, Aust SD. 1978. Microsomal lipid peroxidation. Methods Enzymol 52: 302-306 https://doi.org/10.1016/S0076-6879(78)52032-6
  10. Oliver CN, Ahn BW, Moerman EJ, Goldstein S, Stadtman ER. 1987. Age-related changes in oxidized proteins. J Biol Chem 262: 5488-5491
  11. Peterson GL. 1977. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem 83: 346-356 https://doi.org/10.1016/0003-2697(77)90043-4
  12. Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474 https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  13. Lawrence RA, Burk F. 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Comm 71: 952-958 https://doi.org/10.1016/0006-291X(76)90747-6
  14. Fletcher BL, Dillard CJ, Tappel AL. 1973. Measurement of fluorescent lipid peroxidation products in biological systems and tissues. Anal Biochem 52: 1-9 https://doi.org/10.1016/0003-2697(73)90327-8
  15. Baba N, Radwan H, Itallie TH. 1992. Effects of casein versus soyprotein diets on body composition and serum lipid levels in adult rats. Nutr Res 12: 279-288 https://doi.org/10.1016/S0271-5317(05)80733-X
  16. Nagaoka S, Kanamaru Y, Kuzuya Y, Kojima T, Kuwata T. 1992. Competitive studies on the serum cholesterol lowering action of whey protein and soybean protein in rats. Biosci Biotechnol Biochem 56: 1484-1485 https://doi.org/10.1271/bbb.56.1484
  17. Sarkar PK, Tamang JP. 1995. Changes in the microbial profile and proximate composition during natural and controlled fermentations of soybeans to produce kinema. Food Microbiol 12: 317-325 https://doi.org/10.1016/S0740-0020(95)80112-X
  18. Kiers JL, Van laeken AEA, Rombouts FM, Nout MJR. 2000. In vitro digestibility of Bacillus fermented soya bean. Int J Food Microbiol 60: 163-169 https://doi.org/10.1016/S0168-1605(00)00308-1
  19. Martin GM, Austad SN, Johnson TE. 1996. Genetic analysis of ageing: role of oxidative damage and environmental stresses. Nat Genet 13: 25-34 https://doi.org/10.1038/ng0596-25
  20. Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stadtman ER. 1990. Determination of carbonyl content in oxidatively modified proteins. Meth Enzymol 186: 464-478 https://doi.org/10.1016/0076-6879(90)86141-H
  21. Sohal RS, Brunk UT. 1989. Lipofuscin as an indicator of oxidative stress and aging. Adv Exp Med Biol 266: 17-26
  22. McCord JM, Fridovich I. 1969. Superoxide dismutase. An enzyme function for erythrocuprein. J Biol Chem 24: 6049-6055
  23. Lu LJ, Lin SN, Grady JJ, Nagamani M, Anderson KE. 1996. Altered kinetics and extent of urinary daidzein and genistein excretion in women during chronic soya exposure. Nutr Cancer 26: 289-302 https://doi.org/10.1080/01635589609514485
  24. Wang H, Murphy PA. 1994. Isoflavone contnent of commercial soybean foods. J Agric Food Chem 42: 1666-1677 https://doi.org/10.1021/jf00044a016
  25. Fukutake M, Takahashi M, Ishida K, Kawamura H, Sugimura T, Wakabayashi K. 1996. Quantification of genistein and genistin in soybeans and soybean products. Food Chem Toxicol 34: 457-461 https://doi.org/10.1016/0278-6915(96)87355-8
  26. Hutchins AM, Slavin JL, Lampe JW. 1995. Urinary isoflavonoids phytoestrogen and lignan excretion after consumption of fermented and unfermented soy products. J Am Diet Assoc 95: 545-551 https://doi.org/10.1016/S0002-8223(95)00149-2
  27. Setchell KDR, Adlercreutz H. 1988. Mammalian lignans and phyto-oestrogens: recent studies on their formation, metabolism and biological role in health and disease. In Role of got flora in toxicity and cancer. Rowland IR, ed. Academic Press Limited, San Diego, CA, USA. p 315-345
  28. Lund T, Munson DJ, Haldy ME, Setchell KDR, Lephart ED, Handa RJ. 2003. Equol has unique potent anti-androgen actions. Proceeding of $5^{th}$ International symposium on the role of soy in preventing and treating chronic disease. Sep 21-24, Orlando, FL, USA. p15
  29. Setchell KDR, Borriello SP, Kirk DN, Axelson M. 1984. Nonsteroidal estrogens of dietary origin: Possible role in hormone-dependent disease. Am J Clin Nutr 40: 569-578 https://doi.org/10.1093/ajcn/40.3.569
  30. Halliwell B, Gutteridge JMC. 1981. Formation of a thiobarbituric acid reactive substance from deoxyribose in the presence of iron salts. FEBS Letters 128: 347-352 https://doi.org/10.1016/0014-5793(81)80114-7
  31. Iwai K, Nakaya N, Kawasaki Y, Matsue H. 2002. Antioxidative functions of natto, a kind of fermented soybeans: Effect on LDL oxidation and lipid metabolism in cholesterol-fed rats. J Agric Food Chem 50: 3597-3601 https://doi.org/10.1021/jf0117199
  32. Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissues by hiobarbituric acid reaction. Anal Biochem 95: 351-358 https://doi.org/10.1016/0003-2697(79)90738-3
  33. Aebi H. 1984. Catalase in vitro. Methods Enzymol 105: 121-126 https://doi.org/10.1016/S0076-6879(84)05016-3
  34. Choi JH, Kim DI, Park SH, Kim DW, Lee JS, Kim HS. 1999. Investigation of anti-aging effect and determination of chemical structures of pine needle extract (PNE) through the animal experiments. 1. Effects of PNE on oxygen radicals and their scavenger enzymes in liver of SD rats. Korean J Life Science 9: 466-472
  35. Madani S, Prost J, Belleville J. 2000. Dietary protein level and origin (casein and highly purified soybean protein) affect hepatic storage, plasma lipid transport, and antioxidative defense status in the rat. Nutrition 16: 368-375 https://doi.org/10.1016/S0899-9007(00)00237-9
  36. Izumi T, Piskula MK, Osawa S. 2000. Soy isoflavone aglycones are absorbed faster and in higher amounts than their glucosides in humans. J Nutr 130: 1695-1699 https://doi.org/10.1093/jn/130.7.1695
  37. Setchell KDR. 2003. Equol-origins, actions and clinical relevance of this specific soy isoflavone metabolite. Proceeding of $5^{th}$ International symposium on the role of soy in preventing and treating chronic disease. Sep 21-24, Orlando, FL, USA. p14

Cited by

  1. Effect of Cheonggukjang made with germinated soybean on lipid contents and fecal excretion of neutral steroids in rats fed a high cholesterol diet vol.22, pp.1, 2013, https://doi.org/10.1007/s10068-013-0003-2