The Effects of Fermentated Ephedra sinica on Obese Rats Fed by High Fat Diet

발효마황이 고지방식이로 유발된 비만 흰쥐에 미치는 효과

  • Shin, Young-Jin (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Guk University) ;
  • Kim, Ho-Jun (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Guk University) ;
  • Lee, Myeong-Jong (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Guk University) ;
  • Keum, Dong-Ho (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Guk University)
  • 신영진 (동국대학교 한의과대학 한방재활의학과교실) ;
  • 김호준 (동국대학교 한의과대학 한방재활의학과교실) ;
  • 이명종 (동국대학교 한의과대학 한방재활의학과교실) ;
  • 금동호 (동국대학교 한의과대학 한방재활의학과교실)
  • Received : 2009.09.16
  • Accepted : 2009.10.14
  • Published : 2009.10.31
  • 22 0


Abstract

Objectives : This study was performed to investigate the effects of fermented Ephedra sinica (FMH) nano-extract compared Ephedra sinica (MH) on 3T3 L1 cell viability, lipid and glycometabolism, Obes rats' behavior and state-trait anxiety. Methods and materials : Each of 6 rats was divided into a normal diet, HFD, MH, and FMH. We fed a HFD group of rats a high fat diet and administered normal saline for 8 weeks. And we fed an experimental group of rats a high fat diet and administered an extract of Ephedra sinica and fermentated Ephedra sinica for 8 weeks. At the end of the experiment, the rats were sacrificed to determine their chemical composition. Ephedra sinica and fermentated Ephedra sinica was examined in effects of 3T3 L1 cell viability, lipid and glycometabolism, rats' behavior and state-trait anxiety. Results : 1. Ephedra sinica and fermentated Ephedra sinica didn't induce cytotoxicity at 3L3 L1 cell. 2. Fermentated Ephedra sinica by Lactococcus confusus inhibit $PPAR-{\gamma}$ activation and promote $TNF-{\alpha}$ activation. 3. In MH and FMH group, the body weight and FER decrease occured significantly than in HFD group. 4. No side effect of Ephedra sinica appeared in FMH group, so fermentated Ephedra sinica have a capacity of anti-anxiety. 5. Serum total cholesterol, triglyceride, HDL-cholesterol and creatine of HFD+Ephedra sinica, HFD+LO group were decreased compared with that of HFD group. Decreasing rates of sample group was significant(p<0.05). 6. In both MH and FMH group serum HDL was increased(P>0.05). 7. In MH group only TG was decreased, but in FMH group total cholesterol, TG, and glucose were decreased(P>0.05). Conclusions : Fermentated Ephedra sinica by Lactococcus confusus was superior to Ephedra sinica in capacity of anti-obesity. Further fermentated Ephedra sinica have no Ephedra sinica's side effect.

Keywords

Fermentated Mahuang;Mahuang;Obesity;Ephedra sinica

Acknowledgement

Supported by : 한국연구재단

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