The Effect of KangwhalSokdan-tang(Jianghuoxuduan-tang) Administration on Muscle Regeneration in Atrophied Rat Muscle Induced by Hindlimb Suspension

후지체공(後肢滯空) 유발 흰쥐의 위축 근육에 대한 강활속단탕(羌活續斷湯)의 근 재생효과

  • Soh, Kab-Seog (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Guk University) ;
  • Park, Young-Hoi (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Guk University) ;
  • Oh, Jae-Keun (Dept. of Sport Medicine, Korean National Sport University) ;
  • Lee, Myeong-Jong (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Guk University)
  • 소갑석 (동국대학교 한의과대학 한방재활의학과교실) ;
  • 박영회 (동국대학교 한의과대학 한방재활의학과교실) ;
  • 오재근 (한국체육대학교 스포츠의학교실) ;
  • 이명종 (동국대학교 한의과대학 한방재활의학과교실)
  • Received : 2009.09.16
  • Accepted : 2009.10.14
  • Published : 2009.10.31
  • 12 0


Abstract

Objectives : This experimental study was designed to investigate the effect of KangwhalSokdan-tang(Jianghuoxuduan-tang) on the muscle regeneration of atrophied rat muscle by hindlimb suspension. Materials and methods : In this study, Sprague-Dawley rats weighing about 250g were subjected to hindlimb suspension and divided into total four groups: Normal group(n=6), Control group(n=6), Hindlimb non-treatment group(n=6), Hindlimb treatment group(n=6). Experiments were seperately tried two times. The first trial was studied by the following two groups; The first was normal group(n=6). The second was group(n=18) for hindlimb suspension during 2 weeks (control I group). The second trial after 2 weeks hindlimb suspension was studied by the following three groups; The third group(n=6) was expired immediately after 2 weeks hindlimb suspension. The forth group(n=6) was given free activity during 2 weeks after 2 weeks hindlimb suspension. The fifth group(n=6) was administrated of KST during 2 weeks after 2 weeks hindlimb suspension. In order to investigate degree of muscle atrophy, body weight and gastrocnemius muscle mass were compared. To analyze muscle regeneration factors(expression of IGF-1, Myogenin, MyoD), Western blot was used. Results : The results were analyzed by statistical process as follows, 1. In body weight, all hindlimb suspension groups were lower than normal group, but tendency of increase was shown in KST group compared to non-treatment group after 2 weeks hindlimb suspension. 2. In gastrocnemius muscle mass, KST group on both side was significantly higher than non-treatment group after 2 weeks hindlimb suspension. 3. In case of IGF-I, Type I of KST group was significantly increased than non-treatment group, but Type II was not shown significance. 4. There was no significantly difference in Myogenin. 5. In MyoD, Type I of KST group was significantly increased than control group, and Type II of KST group was significantly increased than non-treatment group. Conclusions : In summary, this study demonstrates that KST administration has an effect to prevent muscle atrophy and contribute muscle regeneration and proliferation. And also it is suggested that IGF-I and MyoD is major factors of myogenesis expression to KST adminstration after hindlimb suspension.

Keywords

KangwhalSokdan-tang(Jianghuoxuduan-tang);Muscle atrophy;Muscle egeneration;Hindlimb suspension;IGF-I;Myogenin;MyoD

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