A Study on the Effect of Changchuldointanggami-bang(Cāngzhútáoréntāngjiāwèi-fāng) in Rheumatoid Arthritis

창출도인탕가미방(蒼朮桃仁湯加味方)이 류마토이드 관절염에 미치는 실험적 연구

  • Park, Eun-Sook (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dae-Jeon University) ;
  • Yoon, Il-Ji (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dae-Jeon University)
  • 박은숙 (대전대학교 한의과대학 한방재활의학과교실) ;
  • 윤일지 (대전대학교 한의과대학 한방재활의학과교실)
  • Received : 2009.07.15
  • Accepted : 2009.10.14
  • Published : 2009.10.31
  • 22 0


Abstract

Objectives : This study was carried out to understand the immune responses of the Changchuldointanggami-bang(after referred to CDIT) on antioxidants, THP-1 cell and rheumatoid arthritis in Collagen-induced Arthritis(CIA) mice. Methods : The antioxidative effect of CDIT on DPPH free radical and SOD-like activity was measured. CDIT was administered to THP-1 cell, cytokine and mRNA associated with inflammation were measured. CDIT was orally administered to mice with arthritis by collagen II and then cytokine, PBMC in the serum were measured. Results : 1. The scavenging activity of CDIT on DPPH free radical was dose-dependent. 2. The effect of CDIT on SOD-like activity was dose-dependent. 3. The production of $IL-1{\beta}$, IL-6, $TNF-{\alpha}$ was decreased significantly in THP-1 cell. 4. The expression of $IL-1{\beta}$ mRNA, IL-6 mRNA, $TNF-{\alpha}$ mRNA were decreased significantly in THP-1 cell. 5. $IL-1{\beta}$, IL-6, $TNF-{\alpha}$ were decreased significantly in the serum of CIA mice. 6. CD3+, CD4+, CD8+ were increased significantly, but CD3+/CD69+, CD3+/CD49b+, B220+/CD23+ were decreased significantly in PBMC of CIA mice. Conclusions : Taking all these observations into account, CDIT is considered to be effective in rheumatoid arthritis. Therefore we have to survey continuously in looking for the effective substance and mechanism in the future.

Keywords

Changchuldoin-tanggamibang($C{\bar{a}}ngzh{\acute{u}}t{\acute{a}}or{\acute{e}}nt{\bar{a}}ngji{\bar{a}}w{\grave{e}}i-f{\bar{a}}ng$, CDIT);Antioxidative effect;Collagen induced Arthritis(CIA);Cytokine

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