DOI QR코드

DOI QR Code

In vitro plant regeneration from axillary buds of Hibiscus syriacus L.

  • Jeon, Seo-Bum (Department of Environmental Horticulture, The University of Seoul) ;
  • Kang, Seung-Won (Gene Research Center, Graduate School of Life and Environmental Science, University of Tsukuba) ;
  • Kim, Wan-Soon (Department of Environmental Horticulture, The University of Seoul) ;
  • Lee, Gung-Pyo (Department of Applied Plant Science, College of Industrial Science, Chung-Ang University) ;
  • Kim, Sun-Hyung (Department of Environmental Horticulture, The University of Seoul) ;
  • Seo, Sang-Gyu (Department of Environmental Horticulture, The University of Seoul)
  • Published : 2009.06.30

Abstract

Presently, we report a simple, reproducible and high frequency plant regeneration in Hibiscus syriacus L. using axillary buds. H. syriacus was regenerated from axillary buds directly or through a callus phase. Regenerated shoots were directly induced from young and fresh axillary buds cultured on Murashige and Skoog medium (MS) supplemented with 0.01 mg/L of the growth regulator thidiazuron (TDZ) after 2 weeks of culture. Directly induced shoots were transferred to hormone-free MS medium and root development was observed after 6 weeks. On the other hand, old and stale axillary buds were regenerated to shoots via callus induction on MS medium containing 0.01–2 mg/L TDZ after 4 weeks. A TDZ concentration of 0.01 mg/L was most effective in callus formation. Green callus was transferred to MS medium containing 0.01 mg/L α-naphthalene acetic acid (NAA) and 0.5 mg/L benzylaminopurine (BA). After 4 weeks, callus had developed into multiple shoots. Plantlets were formed from 10 week cultures of single shoots on hormone-free MS medium. Regenerated plantlets were cultured on MS medium for one month and then transferred to pots containing garden soil. Potted plants were acclimatized for one month and grown to maturity under greenhouse conditions. The present study has shown that various concentrations of plant growth regulator can be effective for in vitro plant regeneration of H. syriacus. The direct and indirect regeneration protocol presented here will be useful for understanding the manipulation and propagation of H. syriacus.

Keywords

References

  1. Duong TN, Truong TTA, Nguyen TDH, Nguyen TD, Nguyen TH, Nguyen QT, Nguyen HV (2007) Effect of genotype, explants size, position, and culture medium on shoot generation of Gerbera jamesonii by receptacle transverse thin cell layer culture. Sci Hort 111:146-151 https://doi.org/10.1016/j.scienta.2006.10.008
  2. Hotta M, Ogata K, Nitta A, Hosikawa K, Yanagi M, Yamazaki K (1989) Useful plants of the world. Heibonsha, Yokyo, Japan, pp 1499
  3. Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss Org Cult 33:105-119 https://doi.org/10.1007/BF01983223
  4. Kamal AM, Praveen KS (1992) Regeneration in Phaseolus vulgaris L.: High-frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron. Planta 186:384-389
  5. Kanyand M, Prakash CS, (2007) Evaluation of peanut genotypes for in vitro plant regeneration using thidiazuron. J Biotechnol 130:202-207 https://doi.org/10.1016/j.jbiotec.2007.02.014
  6. Kerns HR, Meyer, M (1986) Tissue culture propagation of Acer × freemanii using thidiazuron to stimulate shoot tip proliferation. Hort Science 21:1209-1210
  7. Lu CY (1993) The use of thidiazuron in tissue culture. In Vitro Cell Dev Biol 29:92-96 https://doi.org/10.1007/BF02632259
  8. Luis PBC, Adriane CMGM, Silvica BRCC, Ana CMB (1999) Plant regeneration from seedling explants of Eucalyptus grandis × E.urophylla. Plant Cell Tiss Org Cult 56:17-23 https://doi.org/10.1023/A:1006283816625
  9. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  10. Pierik RLM, Steegmans HHM, Mays J (1974) Plantlet formation in callus tissues of Anthurium adreanum Lind. Sc. Hort 2:193-198 https://doi.org/10.1016/0304-4238(74)90009-0
  11. Russel JA, McCown BH (1986) Thidiazuron stimulated shoot differentiation from protoplast-derived callus of Populus. Plant Tiss Cell Cult Abstr pp 49
  12. Singh S, Bhatia SK (1988) Shoot proliferation of pear cultures on medium containing thidiazuron and benzylaminopurine. Hort Science 28:803
  13. Yang LJ, Hidaka M, Masaki H, Uozumi T (1995) In vitro plant regeneration from leaf and petiole explants of Hibiscus syriacus L. Plant Tiss Cult Lett 12:173-17 https://doi.org/10.5511/plantbiotechnology1984.12.173
  14. Yoo EH, Yoo YK, Kim KS (1996) Effects of growth regulators, sucrose, light, and medium on callus and shoot formation in shoot-tip culture of Hbiscus syriacus L. 'Honghwarang' J Kor Soc Hort Sci 37:317-323
  15. Elstner EF, Keller G, Paradies I (1983) Contrasting effects of the cotton defoliant thidiazuron and animoethoxyvinylglycine, an inhibitor of ethylene formation, on stomatal aperture and on ethylene formation in bean (Phaseolus vulgaris) leaves. Ber Dtsch Bot Ges 96:461-466
  16. Kim EY, Yoo YK, Kim KS (1998) Effect of thidiazuron on callus and multiple shoot formation in shoot-tip culture of Hbiscus syriacus L. 'Honghwarang'. Kor J Hort Sci Technol. 17:115-117
  17. Mangai KG (2001) Tissue culture studies in some medicinally important plants. Plant Res 4:171-180
  18. Stoutemeyer VT, Britt OK (1965) The behavior of tissue cultures from English and Algerian ivy in different growth phases. Am J Bot 52:805-810 https://doi.org/10.2307/2439761

Cited by

  1. Development of an efficient protocol for high-frequency regeneration system in Hibiscus syriacus L. vol.44, pp.2, 2017, https://doi.org/10.5010/JPB.2017.44.2.164