Antioxidant and α-Glucosidase Inhibitory Activities of the Extract from Sparganium stoloniferum Buch.-Ham. Root and Its Constituent Compounds

  • Xu, Ming Lu (Henan Institute of Science and Technology) ;
  • Wang, Lan (School of Biotechnology, Kangwon National University) ;
  • Hu, Jian He (Henan Institute of Science and Technology) ;
  • Wang, Myeong-Hyeon (School of Biotechnology, Kangwon National University)
  • Published : 2009.12.31


Three compounds, vanillic acid, p-hydroxylcinnamic acid, p-hydroxybenzaldehyde have been isolated from the ethylacetate extract of Sparganium stoloniferum Buch.-Ham roots using silica gel open column chromatography, preparative thin-layer chromatography (pTLC) and reverse phase high performance liquid chromatography. The structures of the compounds were established on the basis of IR, extensive 1D NMR, and MS analyses. The ethylacetate (EtOAc) extract, vanillic acid, and p-hydroxybenzaldehyde showed $\alpha$-glucosidase inhibition activity of 72.71%, 20.13%, and 30.42%, at the concentration of 10 ${\mu}g/mL$, respectively. The EtOAc extract exhibited strong antioxidant activity with an $IC_50$ value of 24.37 ${\mu}g/mL$ against DPPH radical scavenging activity, the vanillic acid, p-hydroxylcinnamic acid, and p-hydroxybenzaldehyde with an $IC_50$ value of 2.10 ${\mu}M$, 1.59 ${\mu}M$, and 2.72 ${\mu}M$ against DPPH, respectively.


  1. Hsu HY. 1986. Oriental Materia Medica. A concise guide. The Oriental Healing Arts Institute, Long Beach, CA, USA. p 485
  2. Jiang-su New Medical Academy. 1997. Zhong-yao da-ci- dian. Shanghai Science and Technology Publisher, Shang- hai, China. p 56-58
  3. Casirola DM, Ferraris RP. 2006. $\alpha$-Glucosidase inhibitors prevent diet-induced increases in intestinal sugar transport in diabetic mice. Metabolism 55: 832-841
  4. Laar FA, Lucassen PL, Akkermans RP, Lisdonk EH, Guy R, Chris VW. 2005. Alpha-glucosidase inhibitors for patients with type 2 diabetes: results from a Cochrane systematic reviews and meta-analysis. Diabetes Care 28: 154- 163
  5. Anand M, Nicole Z, Pauline MR, Timothy MB, Raymond AD. 1998. $\alpha$-Glucosidase inhibitors as potential broad based anti-viral agents. FEBS Letters 430: 17-22
  6. Pistia BG, Hollingsworth RI. 2001. A preparation and screening strategy for glycosidase inhibitors. Tetrahedron 57: 8773-8778
  7. Yen GC, Chen HY. 1995. Antioxidant activity of various tea extracts in relation to their antimutagenicity. J Agric Food Chem 43: 27-37
  8. Huang ZX, Larry D, John PN. 1993. Mechanisms of ferulic acid conversions to vanillic acid and guaiacol by Rhodotorula rubra. J Biol Chem 268: 23954-23958
  9. Kiyomi K, Tomoko H, Makiko H, Tsutao K. 1983. Reaction of p-hydroxycinnamic acid derivatives with nitrite and its relevance to nitrosamine formation. J Agric Food Chem 31: 780-785
  10. Yoshioka T, Inokuchi T, Fujioka S, Kimura Y. 2004. Phenolic compounds and flavonoids as plant growth regulators from fruit and leaf of Vitex rotundifolia. Zeitschrift fur Naturforschung C 59: 509-514
  11. Li Y, Wen S, Kota BP. 2005. Punica granatum flower extract, a potent alpha-glucosidase inhibitor, improve postprandial hyperglycemia in Zucker diabetic fatty rats. J Ethnopharmacol 99: 239-244
  12. Jayantrao S, Tony T, Michael B, Naveen K, Bo H, Sunil K, Jessica P, William Z. 2008. $\alpha$--Glucosidase inhibitory activity of Syzygium cumini (Linn) Skeels seed kernel in vitro and in Goto-Kaizaki (GK) rats. Carbohydrate Res 343: 1278-1281
  13. Kihara Y, Ogami Y, Tabaru A, Unoki H, Otsuki M. 1997. Safe and effective treatment of diabetes mellitus associated with chronic liver diseases with an alpha-glucosidase inhibitor, acarbose. J Gastroenterol 32: 777-782
  14. Carrascosa M, Pascual F, Aresti S. 1997. Acarbose-induced acute severe hepatotoxicity. Lancet 349: 698-699
  15. Gloster TM, Meloncelli P, Stick RV. 2007. Glycosidase inhibition: an assessment of the binding of eighteen putative transition-state mimics. J Am Chem Soc 129: 2345- 2354
  16. Shyamala BN, Madhava NM, Sulochanamma G, Srinivas P. 2007. Studies on the antioxidant activities of natural vanilla extract and its constituent compounds through in vitro models. J Agric Food Chem 55: 7738-7743
  17. Huang SM, Hsu CL, Chuang HC, Shih PH, Wu CH, Yen GC. 2008. Inhibitory effect of vanillic acid on methylglyoxal-mediated glycation in apoptotic Neuro-2A cells. Neurotoxicology 29: 1016-1022
  18. Wiernsperger NF. 2003. Oxidative stress as a therapeutic target in diabetes: revisiting the controversy. Diabetes Metab 29: 579-585
  19. Tomohiro I, Misato K, Fumihiko H, Yukio F. 2009. Hypoglycemic effect of hot-water extract of adzuki (Vigna angularis) in spontaneously diabetic KK-A mice. Nutrition 25: 134-141
  20. Ihara Y, Toyokuni S, Uchida K, Odaka H, Tanaka T, Ikeda H. 1999. Hyperglycemia causes oxidative stress in pancreatic $\beta$-cell of GK rats, a model of type 2 diabetes. Diabetes 48: 927-932
  21. Ihara Y, Toyokuni S, Uchida K, Odaka H, Tanaka T, Ikeda H. 1999. Hyperglycemia causes oxidative stress in pancreatic $\beta$-cell of GK rats, a model of type 2 diabetes. Diabetes 48: 927-932

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