Anti-obesity Effect of Berberine in Mice Fed a High Fat Diet

  • Hwang, Kwang-Hyun (University of Science and Technology) ;
  • Ahn, Ji-Yun (Functional Food Technology Research Group, Korea Food Research Institute) ;
  • Kim, Sun-A (Functional Food Technology Research Group, Korea Food Research Institute) ;
  • Ha, Tae-Youl (Functional Food Technology Research Group, Korea Food Research Institute)
  • Published : 2009.12.31


We investigated the anti-obesity effect of berberine in mice fed a high fat diet and focused on the analysis of adipogenesis in epdidymal adipose tissue. Male C57BL/6J mice were divided into three groups, which were fed either a normal diet (Nor), a high fat diet (HFD), or a high fat diet plus orally administered berberine (0.2 g /kg body weight) (HFD+B) for 8 weeks. Relative to mice in the HFD group, mice in the HFD+B group showed significant reductions in weight gain and adipose tissue weight. Serum triglyceride levels in mice from the HFD+B group were significantly lower than those of the HFD mice, as were the levels of serum insulin and leptin. An effect of berberine to reduce epididymal adipose mass was revealed by H&E staining. Berberine inhibited the high fat diet-induced increase in levels of the proteins CD36 and CCAAT/enhancer-binding protein $\alpha$ ($C/EBP{\alpha}$) observed in epididymal adipose tissues of mice from the HFD group. These results suggest that berberine has an anti-obesity effect in mice and that the effect is mediated by inhibition of adipogenesis.


  1. Guilbert JJ. 2002. The World Health Report 2002- Reducing risks, promoting healthy life. Education for Health 16: 1-230
  2. Kim DM, Ahn CW, Nam SY. 2005. Prevalence of obesity in Korea. Obes Rev 6: 117-121
  3. Pi-Sunyer FX, Becker DM, Bouchard C. 1998. NHLBI obesity education initiative expert panel on the identification, evaluation, and treatment of overweight and obesity in adults. Obes Res 6: 51S-209S
  4. Waldron A. 2007. Obesity and obesity-related complications screening of adults and children in the primary care office. Northeast Florida Medicine 58: 21-24
  5. Saltiel AR, Kahn CR. 2001. Insulin signalling and the regulation of glucose and lipid metabolism. Nature 414: 799- 806
  6. Grundy SM. 2004. Obesity, metabolic syndrome, and cardiovascular disease. J Clin Endocrinol Metab 89: 2595- 2600
  7. Tilg H, Moschen AR. 2006. Adipocytokines: mediators linking adipose tissue, inflammation and immunity. Nat Rev Immunol 6: 772-783
  8. Anghel SI, Wahli W. 2007. Fat poetry: a kingdom for PPAR gamma. Cell Res 17: 486-511
  9. Cooke D, Bloom S. 2006. The obesity pipeline: current strategies in the development of anti-obesity drugs. Nat Rev Drug Discov 5: 919-931
  10. Ikram M. 1975. A review on the chemical and pharmacological aspects of genus Berberis. Planta Med 28: 353- 358
  11. Subbaiah TV, Amin AH. 1967. Effect of berberine sulphate on Entamoeba histolytica. Nature 215: 527-528
  12. Kong WJ, Zhang H, Song DQ, Xue R, Zhao W, Wei J, Wang YM, Shan N, Zhou ZX, Yang P, You XF, Li ZR, Si SY, Zhao LX, Pan HN, Jiang JD. 2009. Berberine reduces insulin resistance through protein kinase C-dependent up-regulation of insulin receptor expression. Metabolism 58: 109-119
  13. Huang C, Zhang YB, Gong ZW, Sheng XY, Li ZM, Zhang W, Qin Y. 2006. Berberine inhibits 3T3-L1 adipocyte differentiation through the PPAR gamma pathway. BBRC 348: 571-578
  14. Choi BH, Ahn IS, Kim YH, Park JW, Lee SY, Hyun CK, Do MS. 2006. Berberine reduces the expression of adipogenic enzymes and inflammatory molecules of 3T3-L1 adipocyte. Exp Mol Med 38: 599-605
  15. Cho IJ, Ahn JY, Kim S, Choi MS, Ha TY. 2008. Resveratrol attenuates the expression of HMG-CoA reductase mRNA in hamsters. BBRC 367: 190-194
  16. Zhou JY, Zhou SW, Zhang KB, Tang JL, Guang LX, Ying Y, Xu Y, Zhang L, Li DD. 2008. Chronic effects of berberine on blood, liver glucolipid metabolism and liver PPARs expression in diabetic hyperlipidemic rats. Biol Pharm Bull 31: 1169-1176
  17. Unger RH. 2003. Lipid overload and overflow: metabolic trauma and the metabolic syndrome. Trends Endocrinol Metab 14: 398-402
  18. Tang J, Feng Y, Tsao S, Wang N, Curtain R, Wang Y. 2009. Berberine and Coptidis rhizoma as novel antineoplastic agents: a review of traditional use and biomedical investigations. J Ethnopharmacol 126: 5-17
  19. Yin J, Gao Z, Liu D, Liu Z, Ye J. 2008. Berberine improves glucose metabolism through induction of glycolysis. Am J Physiol Endocrinol Metab 294: E148-E156
  20. Febbraio M, Abumrad NA, Hajjar DP, Sharma K, Cheng W, Pearce SF, Silverstein RL. 1999. A null mutation in murine CD36 reveals an important role in fatty acid and lipoprotein metabolism. J Biol Chem 274: 19055-19062
  21. Ramji DP, Foka P. 2002. CCAAT/enhancer-binding proteins: structure, function and regulation. Biochem J 365: 561-575
  22. Olofsson LE, Melander MO, William-Olsson L, Sjostrom KSL, Groop L, Carlsson B, Carlsson LMS, Olsson B. 2008. CCAAT/enhancer binding protein (C/EBP) in adipose tissue regulates genes in lipid and glucose metabolism and a genetic variation in C/EBP is associated with serum levels of triglycerides. J Clin Endocrinol Metab 93: 4880-4886

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