Evaluation of Dry Rehydratable Film Method for Detection of Coliform Bacteria and Escherichia coli

대장균군과 대장균의 검출을 위한 건조필름 방법의 평가

  • Park, Heon-Kuk (Dept. of Food and Nutrition, Dongnam Health College)
  • 박헌국 (동남보건대학 식품영양과)
  • Published : 2009.12.31

Abstract

The adaptability of dry rehydratable film for the qualitative evaluation of coliform bacteria and Escherichia coli was tested. In general, culture methods that employ lactose broth or desoxycholate lactose agar are used for qualitative tests of coliform bacteria. Culture using lactose broth showed a high detection yield and low selectivity when compared to the dry rehydratable film. However, culture methods that employ lactose broth required a long time(48 hrs) for qualitative tests of coliform bacteria and complicated procedures were required to prepare the medium. The detection of coliforms using desoxycholate lactose agar had a slightly higher selectivity than the dry rehydratable film method, but this difference was not statistically significant. However, the preparation of the desoxycholate lactose agar was complicated. EC broth for the detection of E. coli showed the highest detection yield and lowest selectivity; however, this method required complicated procedures for preparation of the medium as well. Overall, the dry rehydratable film had a slightly lower detection yield than the other methods. The detection yield of dry rehydratable film method was over 37.1% at a concentration of 1 cfu/$m{\ell}$. Additionally, the dry rehydratable film method showed high selectivity and did not require preparation. However, because the selectivity of the dry rehydratable film was high, it took a long time(36 hrs) to detect E. coli. Overall, these findings indicate that dry rehydratable film can be used for qualitative detection of coliforms and E. coli.

References

  1. Nelson CL, Fox TL, Busta FF. 1984. Evaluation of dry medium film (Petrifilm VRB) for coliform enumeration. J Food Prot 47:520-525 https://doi.org/10.4315/0362-028X-47.7.520
  2. Matner RR, Fox TL, McIver DE, Curiale MS. 1990. Efficacy of Petrifilm E. coli count plates for E. coli and coliform enumeration. J Food Prot 53:145-150 https://doi.org/10.4315/0362-028X-53.2.145
  3. Ginn RE, Packard VS, Fox TL. 1986. Enumeration of total bacteria and coliforms in milk by dry rehydratable film methods: Collaborative study. J Assoc Off Anal Chem 69:527-531
  4. Curiale MS, Sons T, McIver DE, McAllister JS, Halsey B, Roblee D, Fox TL. 1991. Dry rehydratable films for enumeration of total coliforms and Escherichia coli in foods: Collaborative study. J Assoc Off Anal Chem 74:635-648
  5. Kim KS, Bae EK, Ha SD, Park YS, Mok CK, Hong KP, Kim SP, Park JY. 2004. Evaluation of dry hydratable film method for enumeration of microorganisms in Korean traditional foods. J Food Hyg Safety 19:209-216
  6. Restino L, Lyon RH. 1987. Efficacy of Petrifilm VRB for enumerating coliforms and Escherichia coli from frozen raw beef. J Food Prot 50:1017-1022 https://doi.org/10.4315/0362-028X-50.12.1017
  7. Curiale MS, Fahey P, Fox TL, McAllister JS. 1989. Dry rehydratable films for enumeration of coliform and aerobic bacteria in dairy products: Collaborative study. J Assoc Off Anal Chem 72:312-318
  8. Kwak IS, Chang JK, Lee KS. 1995. Bacterial counts in ginseng products by dry rehydratable film method. J Food Hyg Safety 10:41-43
  9. Cho MH, Bae EK, Ha SD, Park YS, Mok CK, Hong KP, Kim SP, Park JY. 2005. Evaluation of dry hydratable film method for enumeration of microorganisms in meat, dairy and fishery products. Korean J Food Sci Technol 37:294-300
  10. 식품의약품안전청. 2009. 식품공전
  11. Ha SD. 1996. Evaluation of dryfilm method for isolation of microorganisms from foods. Kor J Appl Microbiol Biotechnol 24:178-184
  12. AOAC. 1990. Official Method of Analysis. 15th ed. Arlington, VA