Effects of Culture Conditions of Rhizopus sp. ZB9 on the Production of Saccharifying Amylase during the Preparation of Rice Koji

쌀 입국 제조시 Rhizopus sp. ZB9의 배양조건이 당화 아밀라아제 생성에 미치는 영향

  • Published : 2009.12.31


This study was conducted to determine the influence of cultural conditions such as temperature, time, water content, koji-thickness and agitation on the production of saccharifying amylase by Rhizopus sp. ZB9 isolated from Korean Nuruk during the preparation of rice koji, which is used in brewing Korean rice wines, Takju and Yakju. Rice kojies were made under different cultural conditions, and the saccharifying activities of each koji were tested. The temperature range suitable for the production of saccharifying amylase was $28{\sim}36^{\circ}C$. Based on the saccharifying activity and color, 60 hours of cultivation at $28^{\circ}C$ was believed to produce the optimum results. The water contents of steamed rice suitable for the production of saccharifying amylase were 35~40%. An increase in koji-thickness induced no adverse effects on the production of saccharifying amylase, but agitation-work during cultivation had a harmful effect.


  1. 국세청. 1975. 국 또는 종국 관리규정. 국세청 훈령 제112호. 1975년 2월 10일 공포
  2. Park JW, Lee KH, Lee CY. 1995. Identification of filamentous molds isolated from Korean traditional Nuruk and their amylolytic activities. Korean J Appl Microbiol Biotechnol 23:737-746
  3. So MH. 1993a. Conditions for the production of amylase and protease in making wheat flour Nuruk by Rhizopus japonicus T2. Korean J Food & Nutr 6:96-102
  4. So MH, Lee YS, Han SH, Noh WS. 1999b: Analysis of flavor compounds in Takju mash brewed with a modified Nuruk. Korean J Food & Nutr 12:421-426
  5. Yu TS, Kim HS, Hong J, Ha HP, Kim TY, Yoon IW. 1996. Bibliographical study on microorganisms of Nuruk. J Korean Soc Food Nutr 25:170-179
  6. 北原 覺雄, 久留 島通俊. 1949. 絲狀菌のDiastase組成に關する 硏究. 日本醱酵工學會誌 27:182-183
  7. 국세청. 1990. 국세청기술연구소 주류분석규정. 국세청훈령 제743호. 1990년 11월 28일 공포
  8. So MH. 1993b. Cultural conditions for the production of saccharogenic amylase during rice-koji making by Aspergillus awamori var. kawachii. Korean J Food & Nutr 6:294-300
  9. So MH. 1993c. Conditions for the production of amylase and protease in making wheat flour Nuruk by Aspergillus oryzae L2. Korean J Food & Nutr 6:89-95
  10. So MH, Lee YS, Noh WS. 1999c. Improvement in the quality of Takju by a modified Nuruk. Korean J Food & Nutr 12:427-432
  11. 이성우. 1988. 한국 전통발효식품의 역사적 고찰. 한국 전통 발효식품 연구의 현황과 전망 심포지움. pp.1-12. 한국산업미생물학회, 한국식문화학회, 한국식품과학회
  12. 박록담. 2009. 만인의 술이라야. 전통주 산업 진흥 및 명품화 전략을 위한 세미나. pp.25-33. 한국전통주진흥협회
  13. 주현규, 조광연, 박충균, 조규성, 채수규, 마상조. 1995. 식품 분석법. pp.290-293. 학문사
  14. So MH, Lee YS, Noh WS. 1999a. Changes in microorganisms and main components during Takju brewing by a modified Nuruk. Korean J Food & Nutr 12:226-232
  15. 김종실. 2009. 우리술 산업 경쟁력 강화 방안. 전통주 산업 진흥 및 명품화 전략을 위한 세미나. pp.5-24. 한국전통주진흥협회
  16. 식품의약품안전청. 2009. 식품첨가물공전. http://www.kfda.go.kr. 2009. 10. 30 방문
  17. 정동효. 1974. 발효와 미생물공학. pp.189-245. 선진문화사
  18. So MH, Lee YS. 2003. Takju making with rice koji of Rhizopus sp. and Aspergillus kawachii. Research Note of Zymbio Institute. pp.5-10
  19. Youn BH, Park YJ, Lee SK. 1974. Studies on the formation of organic acid and saccharifying amylase in koji culture by Aspergillus shirousamii U2. Korean J Food Sci Technol 6:127-132
  20. Rha KY. 1989. Seed mold, important in brewing. Korean J Food & Nutr 1:108-110
  21. So MH. 1999. Characteristics of a modified Nuruk made by inoculation of traditional Nuruk microorganisms. Korean J Food & Nutr 12:219-225
  22. Lee SB, Choe KH, Im DS, Kim DC. 1969. Studies on improvement of manufacturing method of enzymic source for Makkulli brewing. Report of the Technical Research Institute of Tax Office 2:62-69
  23. 이계호. 1994. 한국 약주 탁주의 특성과 신기술. 주류산업의 현황과 신기술 개발 심포지움. pp.51-73. 한국산업미생물학회
  24. Kim CJ, Oh MJ, Lee JS. 1985. Studies on digestion of raw starch by Rhizopus oryzae. Korean J Appl Microbiol Bioeng 13:329-337