Inhibitory Activity of Brine Mineral Water on Cancer Cell Growth, Metastasis and Angiogenesis

해양성 광천수의 암세포 성장, 전이 및 신생 혈관 생성 억제 효과

  • Kim, Wan-Jae (Dept. of Biotechnology, College of Biomedical & Health Science, Konkuk University) ;
  • Li, Hua (Dept. of Biotechnology, College of Biomedical & Health Science, Konkuk University) ;
  • Yoon, Taek-Joon (Dept. of Food & Nutrition, Division of Natural Science, Yuhan University) ;
  • Sim, Jae-Man (Gangneung Science Industry Foundation) ;
  • Choi, Seon-Kang (Gangneung Science Industry Foundation) ;
  • Lee, Kwang-Ho (Dept. of Biotechnology, College of Biomedical & Health Science, Konkuk University)
  • 김완재 (건국대학교 의료생명대학 생명공학과) ;
  • 리화 (건국대학교 의료생명대학 생명공학과) ;
  • 윤택준 (유한대학 식품영양과) ;
  • 심재만 (강릉과학산업진흥원) ;
  • 최선강 (강릉과학산업진흥원) ;
  • 이광호 (건국대학교 의료생명대학 생명공학과)
  • Published : 2009.12.31

Abstract

Brine mineral water(BMW) has recently gained attention as a new water resource due to its biological activities. In this study, BMW from the Geumjin area(Gangneung-city, Korea) was evaluated for its growth inhibition, anti-metastasis and anti-angiogenesis activity against cancer cells. The in vitro cytotoxicity was measured by CCK assay, and the anti-metastasis activity was estimated by lung metastasis in vivo. The in vitro incubation of mouse splenic cells with BMW that had been diluted more than 4-fold showed no effect on the cell growth when compared to a control group. Additionally, BMW inhibited the growth of the EL-4, L5178Y-R and colon26-M3.1 cancer cell lines in a dose-dependent manner. In vivo evaluation of the anti-metastasis activity of BMW in BALB/c mice inoculated with the colon26-M3.1 cell line revealed dose-dependent inhibition in response to treatment with samples that were diluted by up to 9 times. Finally, treatment with BMW effectively suppressed the growth of vascular endothelial growth factor(VEGF) added human umbilical vein endothelial cells. Overall, these results suggest that BMW has anti-cancer activity.

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