Nutrients Contents in Different Parts of Pickly Pear(Opuntia humifusa) and Possible Anti-Breast Cancer Effect

천년초선인장의 부위별 영양성분 분석과 정상 및 암세포에 미치는 효과

  • Yoon, Jin-A (Dept. of Food and Nutrition, Baewha Women's University) ;
  • Hahm, Sahng-Wook (Division of Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Son, Yong-Suk (Division of Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University)
  • 윤진아 (배화여자대학 식품영양과) ;
  • 함상욱 (고려대학교 생명과학대학 생명공학부) ;
  • 손용석 (고려대학교 생명과학대학 생명공학부)
  • Published : 2009.12.31

Abstract

In order to examine the nutritional value of prickly pear(Opuntia humifusa), contents of ash, protein, fat, minerals and vitamins were determined on freeze-dried stem, fruit, seed and root from plants harvested in autumn. The average moisture contents for stem, fruit, seed and root were 67~87%. Crude ash content determined on dry weight basis was 2~3%. Crude protein existed mostly in seed(2.95%) and root(2.37%). Crude fat was detected mainly in seed(4.49%). Contents of major minerals(mg/100 mg dry weight) was generally higher in stem. Ca in stem(4,142.30) and fruit(2,790.86) were much higher than in seed(43.37). P in stem, seed and fruit were 448.19, 263.20 and 161.59, respectively. Stem also displayed more abundant Mg(1,110.86), Zn(35.62) and Mn(37.07). However, fruit contained higher amounts of Fe(13.38) and Se(0.15). Vitamin A was negligible in all plant parts. Vitamin E contents in fruit and stem were 1.78 mg and 1.22 mg/mg dry weight, respectively. Vitamin C was detected mostly in fruit(445.40) and stem(260.94). Use of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-based microtiter assay of cell viability demonstrated an anti-proliferative effect of O. humifusa extract on the MCF-7 estrogen-dependent human breast cancer cell line.

References

  1. A.O.A.C. 1990. Official Methods of Analysis. 15th, Association of Analytical Chemists, Washington D. C. pp.31
  2. Han IH, Lee KA, Byoun KE. 2007. The antioxidant activity of Korean cactus (Opuntia humifusa) and the quality characteristics of cookies with cactus powder added. Korean J Food Cookery Sci 23:443-451
  3. Kim JE, Park JH, Kang BW, Seo MJ, Choi YH, Lim HS, Seo KI, Kim JI, Joo WH, Lee BK, Jeong YK. 2008. Anticancer activity of ethanol extract from peel of Citrus junos and Poncirus trifoliata on MCF-7 breast cancer cells. J Life Sci 18:1435-1441 https://doi.org/10.5352/JLS.2008.18.10.1435
  4. Lee KS, Kim MG, Lee KY. 2004. Antimicrobial effect of the extracts of cactus Chounnyouncho (Opuntia humifusa) against food borne pathogens. J Korean Soc Food Sci Nutr 33:1268-1272 https://doi.org/10.3746/jkfn.2004.33.8.1268
  5. Rodriguez-Felix A, Cantwell M. 1988. Developmental changes in composition and quality of prickly pear cactus cladodes (nopalitos). Plant Foods Hum Nutr 38:83-93 https://doi.org/10.1007/BF01092314
  6. Askar A, El-Smamhy SK. 1981. Chemical composition of prickly pear fruit. Deutsche Lebensmittel-Rundschau 77:279-281
  7. Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL Abbott BJ, Mayo JG, Shoemaker RH, Boyd MR. 1988. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res 48:589-601
  8. Yoon JA, Son YS. 2009. Effects of fruits and stems of Opuntia ficus-indica on blood glucose and lipid metabolism in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 38:146-153 https://doi.org/10.3746/jkfn.2009.38.2.146
  9. Kuti JO. 1992. Growth and compositional changes during the development of prickly pear fruit. J Horticultural Sci 67:861-868 https://doi.org/10.1080/00221589.1992.11516319
  10. Kim YH, Park SC, Son WY, Rhee MH, Kim TW, Han SH, Kim BH, Kim KS. 2005. Hepatoprotective effects of Opuntia humifusa against carbon tetrachloride-induced acute liver injury in rats. Lab Anim Res 21:263-266
  11. Lee YC, Hwang KH, Han DH, Kim SD. 1997. Compositions of Opuntia ficus-indica. Korean J Food Sci Technol 29:847-859
  12. Yoon JA, Son YS. 2009. Effects of Opuntia ficus-indica complexes B(OCB) on blood glucose and lipid metabolism in streptozotocininduced diabetic rats. Korean J Food & Nutr 22:48-56
  13. Korea Food & Drug Administration. 2008. Functional Food Code. pp.37-105
  14. Oh SI, Lee MS. 2008. Antioxidative and cytotoxic effects of ethanol extracts from Elaeagnus multiflora. Korean J Food & Nutr 21:403-409
  15. Kim YR, Lee MR, Kim YH, Jang BJ, Park SC, Han SH, Kim BH, Ryoo ZY, Kim KS. 2005. Effect of Opuntia humifusa extract on indomethacin-induced gastric ulcer in Sprague Dawley rat. Lab Anim Res 21:375-378
  16. Cho JY, Park SC, Kim TW, Kim KS, Song JC, Kim SK, Lee HM, Sung HJ, Song YB, Yoo ES, Lee CH, Rhee MH. 2006. Radical scavenging and anti-imflammatory activity of extracts from Opuntia humifusa. Raf Jo Pharm Pharmacol 58:113-119 https://doi.org/10.1211/jpp.58.1.0014
  17. Choi HJ, Park SC, Hong TH. 2005. Anti-tumor activity of fermented liquid Opuntia humifusa in cervical cancer cells and its chemical composition. J Korean Soc Food Sci Nutr 34: 1525-1530 https://doi.org/10.3746/jkfn.2005.34.10.1525
  18. Kim HW, No JY, Ko JS. 2007. Anti-oxidant effects of Anemarrhenae rhizoma in three different lineages. Korean J Orient Int Med 28:608-614
  19. 현화진, 송경희, 최미경, 손숙미. 2007. 쉽게 보는 식품칼로리 와 영양성분표. pp.106 & pp.136. 교문사
  20. Kim KS, Ahn MJ, Kim GS, Park SC, Rhee MH, In JG, Kim BH, Kim HH, Han SH. 2007. Effects of Opuntia humifusa extract on DNCB-induced allergic contact dermatits in Balb/c mice. Lab Anim Res 23:169-173
  21. Program Resources, Inc., National Cancer Institute-Frederick Cancer Research Facility, Maryland 21701