Effect of different light sources and ventilation on in vitro shoot growth and rooting of a rare and endangered species, Tsuru-rindo(Tripterospermum japonicum)

희귀 및 멸종위기 식물 덩굴용담의 기내생장에 미치는 광질 및 환기효과

  • Moon, Heung-Kyu (Division of Biotechnology, Korea Forest Research Institute(KFRI)) ;
  • Park, So-Young (Division of Biotechnology, Korea Forest Research Institute(KFRI))
  • 문흥규 (국립산림과학원 생물공학과) ;
  • 박소영 (국립산림과학원 생물공학과)
  • Published : 2008.09.30


Effects of light generated by LEDs on shoot growth and rooting of Tsuru-rindo(Tripterospermum japonicum) were evaluated. Apical shoots(one or two node with 3-4 leaves) were cultured on MS basal medium with 3% sucrose and maintained for four weeks under five different light qualities: fluorescent lamp(F), 100% red LED(R), 70% red LED+30% blue LED(R7B3), 50% red LED+50% blue(R5B5), or 100% blue LED(B). Rooting was promoted by both red light and fluorescent lamp, and the effect was further promoted under the ventilation. Red light enhanced shoot node elongation, whereas blue light appeared to suppress it. Growth of shoots and leaves were enhanced under the ventilation irrespective of the different light qualities. Under the ventilated condition, total fresh weight of plants was highest in R7B3 LED as 257.7 mg per plant. Dry matters, which are used for index of plant growth, were lowest under red light, whereas it was highest under blue light. The dry matter was inclined to getting higher by ascending the ratio of blue light and red light. Total chlorophyll content was highest in both R7B3 LED and R5B5 LED under ventilation as 29.5 and 31.2, respectively. Above results suggest that light quality optimization could be an important factor to foster in vitro growth of the species. Ventilation treatment appeared to be another important factor to induce normal shoot growth and rooting.



  1. Dooley JH (1991) Influence of lighting spectra on plant tissue culture. ASAE (American Society of Agricultural Engineers) Meeting, Chicago, Paper No. 917530
  2. Bang JW, Lee MK, Chung SH (1994) Somatic embryogenesis and plant regeneration in leaf explant cultures of Gentiana scabra var. buergeri. Korean J Plant Tiss Cult 21: 233-237
  3. Bula RJ, Morrow TW, Tibbitts TW, Barta DJ, Ignatius RW, Martin TS (1991) Light-emitting diodes as a radiation sources for plants. HortSci 26: 203-205
  4. Kim JA, Moon HK (2006) Effect of light-emitting diodes (LEDs) and ventilation on the in vitro shoot growth of Eucalyptus pellita. J Korean For Soc 95: 716-722
  5. Heo JW, Lee CW, Chakrabarty D, Paek KY (2002) Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light-emitting diode (LED). Plant Growth Reg. 38: 225-230
  6. Gonzlez A, Arigita L, Majada J, Snchez Tams R (1997) Ethylene involvement in in vitro organogenesis and plant growth of Populus tremula L. Planr Growth Regul. 22: 1-6
  7. Lim JD, Yu CY (2000) Multiple shoot formation of Gentiana axillariflora leveille by in vitro culture. Korean J Medicinal Crop Sci 8: 41-48
  8. Mcree KJ (1972) The action spectra, absorptance and quantum yield of photosynthesis in crop plants. J Agricult Metro 9: 191-196
  9. Lee YM, Lee WR (1997) Illustrated rare and endangered species in Korea. Korea Forest Research Institute, Jungbu Forest Experimental Station, pp 255
  10. Kim SJ, Hahn EJ, Heo JW, Paek KY (2004) Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci Hort 101: 143-151
  11. Nguyen QT, Kozai T (2005) Photoautotrophic micropropagation of woody species. In: T Kozai et al. (eds), Photoautotrophic (sugar-free medium) micropropagation as a new propagation and transplant production system, pp 123-146
  12. Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2003) Response of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell Tiss Org Cult 73: 43-52
  13. Nhut DT, Don NT, Tanaka M (2007) Light-emitting diodes as an effective lighting sources for in vitro banana culture. In: S.M. Jain and Haggman (eds), Protocols for Micropropagation of Woody Trees and Fruits, Springer press, pp 527-541
  14. Poudel PR, Kataoka I, Mochioka R (2008) Effect of red and blue-light-emitting diodes on growth and morphogenesis of grapes. Plant Cell Tiss Org Cult 92: 147-153
  15. Seong NS, Park CH, Lee ST, Kim SM (1993) Plant regeneration and multiplication of Gentiana scabra bunge through leaf and stem culture. Korean J Medicinal Crop Sci 1: 129-136
  16. Seong NS, Park CH, Kim KS, Lee ST, Chang YH (1996) In vitro variant induction and its content of gentiopicroside of Gentiana scabra Bunge. Korean J Medicinal Crop Sci. 3: 40-44
  17. Sung IK, Kitoya M, Hirano T (1998) The effects of time and intensity of supplemental blue lighting during morning twilight on growth and physiological performance of cucumber seedlings. Life Support and Biosphere Sci 5: 137-141
  18. Faisal M, Ahmad N, Anis M (2007) An efficient micropropagation system for Tylophora indica: an endangered, medicinally important plant. Plant Biotechnol Rep 1: 155-161
  19. Kartsonas E, Papafotiou M (2007) Mother plant age and seasonal influence on in vitro propagation of Quercus euboica Pap., an endemic, rare and endangered oak species of Greece. Plant Cell Tiss Org Cult 90: 111-116
  20. Moon HK, Park SY, Kim YW and Kim CS (2006) Growth of Tsuru-rindo (Tripterospermum japonicum) cultured in vitro under various sources of light-emitting diode (LED) irradiation. J Plant Biol 49: 174-179
  21. Muleo R., Morini S, Casano S (2001) Photoregulation of growth and branching of plum shoots: physiological action of two photosystems. In Vitro Cell Dev Biol-Plant 37: 609-617
  22. Muleo R, Morini S (2006) Light quality regulates shoot cluster growth and development of MM 106 apple genotype in in vitro culture. Sci Hort 108(4): 364-370
  23. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15: 473-479
  24. Sanjaya, Muthan B, Rathore TS, Rai VR (2006) Micropropagation of an endangered Indian sandalwood (Santalum album L.). J For Res 11: 203-209
  25. SAS (1989) SAS/STAT User's Guide, Version 6, Ed 4, Vol2. SAS Institute, Cary
  26. Seabrook JEA (2005) Light effects on the growth and morphogenesis of potato (Solanum tuberosum) in vitro: a review. Amer J Potato Res 82: 353-367
  27. Shin KS, Murthy HN, Heo JW, Hahn EJ, Paek KY (2008) The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants. Acta Physiol Plant 30: 339-343
  28. Wellburn AR (1994) The spectral determination of chlorophylls a and b, as well as total carotenoides, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144: 307-313
  29. Zobayed SMA (2005) Ventilation in micropropagation. T. Kozai et al. (eds). Photoautotrophic (sugar-free medium) micropropagation as a new propagation and transplant production system, Springer, pp 147-186

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