In vitro micropropagation of Philodendron cannifolium

기내배양에 의한 Philodendron cannifolium의 대량번식

  • Published : 2008.09.30


In order to micropropagate uniform plantlets of Philodendron cannifolium in vitro, the shoot tips were cultured on MS media supplemented with $0.5{\sim}10.0$ mg/L BA or $0.05{\sim}0.1$ mg/L thidiazuron(TDZ). The adventitious multi-bud clusters from basal part of shoots were formed on MS media containing $2.0{\sim}5.0$ mg/L BA or $0.05{\sim}0.1$ mg/L TDZ. But the shoots grown on MS media with TDZ showed necrosis by the lack of chlorophyll. The adventitious multi-bus clusters were cut into $5{\sim}7$ mm sections and cultured on MS media containing BA and TDZ for shoot proliferation. Shoots were proliferated vigorously on MS medium supplemented with $1.0{\sim}3.0$ mg/L BA with up to 30 shoots. But abnormally swollen hard calli were formed from basal parts of shoots on MS media with TDZ and high concentration of BA(10.0 mg/L). The proliferated shoots on same media also showed necrosis by the lack of chlorophyll. The shoot growth and rooting were favorable on MS media containing $0.5{\sim}2.0$ mg/L IBA. The rooted plantlets were acclimatizated effectively in soil mixed with perlite 1:vermiculite 1 or vermiculite alone. Fifteen mL of liquid medium containing 10 g/L activated charcoal and 30 g/L sucrose were added in same vessels after small shoots were proliferated to stimulate shoot growth and rooting. After 8 weeks in culture, the shoots were dipped into high concentration of IBA solution. and planted in soil mexed with perlite 1:vermiculite 1. The shoot growth and rooting were favorable in dipping treatments of $500{\sim}2,000$ ppm IBA solutions for 10 sec.



  1. Earle ED, Langhans RW (1974) Propagation of Chrysanthemum in vitro. I. Multiple plantlets from shoot tips and the establishment of tissue culture. J Amer Soc Hort Sci. 99: 128-132
  2. Han BH, Joung HY, Ko JY (1997) In vitro propagation of Ficus benjamina by shoot tip culture. J Kor Soc Hort Sci 38: 315-319
  3. Han BH, Yae BW, Goo DH, Yu HJ (2004) Micropropagation of Philodendron wend-imbe through adventitious multi-bud cluster formation. Korean J Plant Biotechnol 31: 115-119
  4. Hackett WP (1985) Juvenility, maturation and rejuvenation in wood plants. Hort Rev 7: 109-158
  5. Fellman CD, Read PE, Hosier MA (1987) Effect of thidiazuron and CPPU on meristem formation and shoot proliferation. HortScience 22: 1197-1200
  6. Kusey WE, Hammer PA, Weiler TC (1980) In vitro propagation of Gypsophila paniculata L. 'Bristol Fairy'. Hort- Science 15: 600-601
  7. Han BH, Yae BW, Yu HJ, Shin JS (2001) In vitro propagation of Spathiphyllum floribundum cv. Cupid. Korean J Plant Tiss Cult 28: 209-213
  8. Murashige T, Skoog F (1962) A revised medium for rapid growth and biossays with tobacco tissue culture. Physiol Plant 15: 473-497
  9. Pierik RLM (1990) Commercial micropropagation in Europe. In: Debergh P, Zimmerman R (eds), Micropropagation, Kluwer Academic Publishers, Dordrecht, pp 155-166
  10. Pennazio S (1975) Effect of adenine and kinetin on development of carnation tips cultured in vitro. J Hort Sci 50: 161-164
  11. Ziv M, Arial T (1991) Bud proliferation and plant regeneration in liquid-cultured Philodendron treated with ancymidol and paclobutrazol. J Plant Growth Regul 10: 53-57
  12. Ziv M, Yogev T, Krebs O (1986) Effects of paclobutrazol and chlomequat on growth pattern and shoot proliferation of normal and variant Aechmea faciata 'Baker' plants regenerated in vitro. Israel J Bot 35: 175-182
  13. Takayama S, Misawa M (1982) Regulation of organ formation by cytokinin and auxin in Lilium bulbscales grown in vitro. Plant Cell Physiol 23: 67-74
  14. Reynolds JF (1987) Chemical regulation in tissue culture; An overview. HortScience 22: 1192-1194
  15. Maene LJ, Debergh PC (1985) Liquid medium addition to established tissue cultures to improve elongation and rooting in vitro. Plant Cell Tissue Organ Cult 5: 23-33
  16. Piqueras A, Han BH, van Huylenbroeck JM, Debergh PC (1998) Effect of different environmental conditions in vitro on sucrose metabolism and antioxidant enzymatic activities in cultured shoots of Nicotiana tabacum L. Plant Growth Regul 25: 5-10

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