DOI QR코드

DOI QR Code

Occurrence Pattern of an Unidentified Moth Captured by Sex Pheromone Trap of the Oriental Fruit Moth, Grapholita molesta, and Its Discriminating Molecular Markers

복숭아순나방(Grapholita molesta) 성페로몬 트랩에 포획된 미동정 나방의 발생패턴과 판별 분자지표

  • Huh, Hye-Jung (School of Bioresource Sciences, Andong National University) ;
  • Son, Ye-Rim (School of Bioresource Sciences, Andong National University) ;
  • Kim, Yong-Gyun (School of Bioresource Sciences, Andong National University)
  • 허혜정 (안동대학교 생명자원과학부 식물의학) ;
  • 손예림 (안동대학교 생명자원과학부 식물의학) ;
  • 김용균 (안동대학교 생명자원과학부 식물의학)
  • Published : 2008.09.30

Abstract

An unidentified moth was captured in sex pheromone traps of the oriental fruit moth, Grapholita molesta, especially at spring season in apple orchards and their vicinity. Though the captured males were similar in appearance to G. molesta males, they were easily distinguished by a matted difference in body size. Their occurrence pattern was also similar to that of overwintering G. molesta population from April to May, at which more males were captured in the pheromone traps installed in the vicinity of apple orchards than within apple orchards. After May, they were no longer captured in the pheromone traps. To investigate any larval damage due to this unidentified moth, molecular markers needed to be developed. Four PCR-RFLP markers originated from cytochrome b region of mitochondrial DNA could distinguish this unidentified moth from G. molesta.

References

  1. Bochert, D.M., R.E. Stinner, J.F. Walgenbach and G.G. Kennedy. 2004. Oriental fruit moth (Lepidoptera: Tortricidae) phenology and management with methoxyfenozide in North Carolina apples. J. Econ. Entomol. 97: 1353-1364 https://doi.org/10.1093/jee/97.4.1353
  2. Boo, K.S. 1998. Variation in sex pheromone composition of a few selected lepidopteran species. J. Asia-Pac. Entomol. 1: 17-23 https://doi.org/10.1016/S1226-8615(08)60003-5
  3. Kanga, L.H.B., D.J. Pree, F.W. Plapp, Jr. and J.L. van Lier. 2001. Sex-linked altered acetylcholinesterase resistance to carbamate insecticides in adults of the oriental fruit moth, Grapholita molesta (Lepidoptera; Tortricidae). Pestic. Biochem. Physiol. 71: 29-39 https://doi.org/10.1006/pest.2001.2562
  4. Ming, Q.L., Y.H. Yan and C.Z. Wang. 2007. Mechanisms of premating isolation between Helicoverpa armigera (Hübner) and Helicoverpa assulta (Guenée) (Lepidoptera: Noctuidae). J. Insect Physiol. 53: 170-178 https://doi.org/10.1016/j.jinsphys.2006.11.007
  5. Pree, D.J., K.J. Whitty, L. van Driel, G.M. Walker and L. Van Driel. 1998. Resistance to insecticides in oriental fruit moth populations (Grapholita molesta) from the Niagara Peninsula of Ontario. Can. Entomol. 130: 245-256 https://doi.org/10.4039/Ent130245-3
  6. Simon, C., F. Frati, A. Beckenbach, B. Crespi, H. Liu and P. Flook. 1994. Evolution, weighting, and phylogenetic utility of mitochondrial gene-sequences and a compilation of conserved polymerase chain-reaction primers. Ann. Entomol. Soc. Am. 87: 651-701 https://doi.org/10.1093/aesa/87.6.651
  7. Ahn, S.B., H.W. Koh and Y.I. Lee. 1985. Study on apple pests and natural enemy. Res. Rept. RDA. Crop Protection: 417-428
  8. Usmani, K.A. and P.W. Shearer. 2001. Topical pheromone trap assays for monitoring susceptibility of male oriental fruit moth (Lepidoptera: Tortricidae) populations to azinphosmethyl in New Jersey. J. Econ. Entomol. 94: 233-239 https://doi.org/10.1603/0022-0493-94.1.233
  9. Kim, Y., M.L. Lee and C. Chung. 1998. Study on the genetic variation of the mitochondrial DNA in the beet armyworm, Spodoptera exigua (Hübner), using PCR-RFLP. Kor. J. Appl. Entomol. 37: 23-30
  10. Rothschild, G.H.L. and R.A. Vickers. 1991. Biology, ecology and control of the oriental fruit moth. pp. 389-412. In World crop pests, Tortricid pests: their biology, natural enemies and control, Vol. 5. eds. by L.P.S. Van der Geest and H.H. Evenhuis. Elsevier, Amsterdam
  11. Jung, S. and Y. Kim. 2008. Comparative analysis to damage reduction of host plant by applying a mating disruptor of the oriental fruit moth, Grapholita molesta, in two different cultivation environments of apple orchard. Kor. J. Appl. Entomol. 47: 51-57 https://doi.org/10.5656/KSAE.2008.47.1.051
  12. Clary, D.O. and D.R. Wolstenholme. 1985. The mitochondrial DNA molecule of Drosophila yakuba: nucleotide sequence, gene organization, and genetic code. J. Mol. Evol. 22: 252-271 https://doi.org/10.1007/BF02099755
  13. Hyun, J.S. 2005. Integrated pest management. Principles and practices. Kor. J. Appl. Entomol. 44: 73-90
  14. Sambrook, J.E., F. Fritsh and T. Maniatis. 1989. Molecular cloning. A laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
  15. Kim, D.S., K.S. Boo and H.Y. Jeon. 2004. Evaluation of pheromone lure of Grapholita molesta (Lepidoptera: Tortricidae) and forecasting its phenological events in Suwon. Kor. J. Appl. Entomol. 43: 281-289
  16. Roehrich, R. 1961. Contribution a l'étude écologique des populations de la tordeuse de pêcher (Grapholita molesta Busk) dans la région Aquitaine. Annales des Epiphyties. p.114
  17. Jung, S., C. Park, M. Park, S. Lee, K. Choi, Y. Hong and Y. Kim. 2006. Efficacy of commercial mating disruptors on field overwintering populations of Oriental fruit moth, Grapholita molesta (Busck). Kor. J. Appl. Entomol. 45: 235-240

Cited by

  1. Simultaneous identification ofGrapholita molestaBusck andGrapholita dimorphaKomai by PCR-RFLP vol.46, pp.3, 2016, https://doi.org/10.1111/1748-5967.12166
  2. Different types of fruit damages of three internal apple feeders diagnosed with mitochondrial molecular markers vol.16, pp.2, 2013, https://doi.org/10.1016/j.aspen.2013.01.008