Effects of cranberry powder on serum lipid profiles and biomarkers of oxidative stress in rats fed an atherogenic diet

  • Kim, Mi-Joung ;
  • Jung, Ha-Na ;
  • Kim, Ki-Nam ;
  • Kwak, Ho-Kyung
  • Published : 2008.09.30


This study investigated that the antioxidative effect of freeze-dried cranberry powder against protein and lipid oxidation and ameliorative effect of serum lipid profile in rat fed atherogenic diet. Six weeks old male Sprague-Dawley rats were divided into the following four groups: normal diet group with 5% com oil(control), atherogenic diet group with 5% com oil, 10% lard, 1% cholesterol, and 0.5% sodium cholate(HFC), atherogenic plus 2% cranberry powder diet group(HFC+C2), and atherogenic plus 5% cranberry powder diet group(HFC+C5), and respective diet and water were fed daily for 6 weeks. After the experimental period, the serum lipid profile, such as total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglyceride, ferric reducing ability of plasma(FRAP), plasma phenolics content, superoxide dismutase(SOD) activity, serum protein carbonyl and thiobarbituric acid reactive substances(TBARS) levels were examined. Total phenolic compound and total flavonoid levels in freeze-dried cranberry powder were 9.94 mg/g and 8.12 mg/g, respectively. Serum total cholesterol and LDL-cholesterol levels were not significantly different for cranberry powder treatment, but serum HDL-cholesterol level was significantly increased in HFC+C5 group compared with HFC group. Plasma FRAP value tended to be increased by cranberry powder treatment though there was no significant difference. Plasma total phenol concentrations and SOD activities were not significantly different among all groups. Serum protein carbonyl and TBARS levels were significantly decreased in HFC+C5 group compared with HFC group. Overall results suggested that freeze-dried cranberry powder might have the serum lipid improving effect, as well as anti oxidative effect demonstrated by its protective effect against protein and lipid oxidation.


Antherogenic diet;protein carbonyl;TBARS;FRAP;cranberry powder


  1. Friedewald WT, Levy RI & Fredrickson DS (1972). Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18:499-502
  2. Gotteland M, Andrews M, Toledo M, Munoz L, Caceres P, Anziani A, Wittig E, Speisky H & Salazar G (2008). Modulation of Helicobacter pylori colonization with cranberry juice and Lactobacillus johnsonii La1 in children. Nutrition 24:421-426 https://doi.org/10.1016/j.nut.2008.01.007
  3. Howell AB, Reed JD, Krueger CG, Winterbottom R, Cunningham DG & Leahy M. (2005). A-type cranberry proanthocyanidins and uropathogenic bacterial anti-adhesion activity. Phytochemistry 66:2281-2291 https://doi.org/10.1016/j.phytochem.2005.05.022
  4. Jia Z, Tang M & Wu J (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxides radicals. Food Chem 64:555-559 https://doi.org/10.1016/S0308-8146(98)00102-2
  5. Kresty LA, Howell AB & Baird M (2008). Cranberry proanthocyanidins induce apoptosis and inhibit acid-induced proliferation of human esophageal adenocarcinoma cells. J Agric Food Chem 56:676-680 https://doi.org/10.1021/jf071997t
  6. Ramiro-Puig E, Urpi-Sarda M, Perez-Cano FJ, Franch A, Castellote C, Andres-Lacueva C, Izquierdo-Pulido M & Castell M (2007). Cocoa-enriched diet enhances antioxidant enzyme activity and modulates lymphocyte composition in thymus from young rats. J Agric Food Chem 55:6431-6438 https://doi.org/10.1021/jf070487w
  7. Reznick AZ & Packer L (1994). Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods Enzymol 233:357-363 https://doi.org/10.1016/S0076-6879(94)33041-7
  8. Ruel G, Pomerleau S, Couture P, Lemieux S, Lamarche B & Couillard C (2006). Favourable impact of low-calorie cranberry juice consumption on plasma HDL-cholesterol concentrations in men. Br J Nutr 96:357-364 https://doi.org/10.1079/BJN20061814
  9. Villarreal A, Stoecker BJ, Garcia C, Garcia K, Rios R, Gonzales C, Mandadi K, Faraji B, Patil BS & Deyhim F (2007). Cranberry juice improved antioxidant status without affecting bone quality in orchidectomized male rats. Phytomedicine 14:815-820 https://doi.org/10.1016/j.phymed.2007.04.004
  10. Shahidi F & Wanasundara PKJPD (1992). Phenolic antioxidants. Crit Rev Food Sci Nutr 32:67-103 https://doi.org/10.1080/10408399209527581
  11. Vattem DA, Randhir R & Shetty K (2005). Cranberry phenolicsmediated antioxidant enzyme response in oxidatively stressed porcine muscle. Process Biochem 40:2225-2238 https://doi.org/10.1016/j.procbio.2004.09.001
  12. Sriram N, Kalayarasan S & Sudhandiran G (2008). Enhancement of antioxidant defense system by epigallocatechin-3-gallate during bleomycin induced experimental pulmonary fibrosis. Biol Pharm Bull 31:1306-1311 https://doi.org/10.1248/bpb.31.1306
  13. Vinson JA, Zubik L, Bose P, Samman N & Proch J (2005). Dried fruits: excellent in vitro and in vivo antioxidants. J Am Coll Nutr 20:44-50
  14. Singleton VL & Rossi JA (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144-158
  15. Vattem DA, Ghaedian R & Shetty K (2005). Enhancing health benefits of berries through phenolic antioxidant enrichment: focus on cranberry. Asia Pac J Clin Nutr 14:120-130. Review
  16. Wolfe KL & Liu RH (2007). Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods, and dietary supplements. J Agric Food Chem 55:8896-8907 https://doi.org/10.1021/jf0715166
  17. Bomser J, Madhavi DL, Singletary K & Smith MA (1996). In vitro anticancer activity of fruit extracts from Vaccinium species. Planta Med 62:212-216 https://doi.org/10.1055/s-2006-957862
  18. Pedersen CB, Kyle J, Jenkinson AM, Gardner PT, McPhail DB & Duthie GG (2000). Effects of blueberry and cranberry juice consumption on the plasma antioxidant capacity of healthy female volunteers. Eur J Clin Nutr 54:405-408 https://doi.org/10.1038/sj.ejcn.1600972
  19. Piljac-Zegarac J, Valek L, Martinez S & Belscak A (2008). Fluctuations in the phenolic content and antioxidant capacity of dark fruit juices in refrigerated storage. Food Chem (Epub ahead of print)
  20. Das S, Santani DD & Dhalla NS (2007). Experimental evidence for the cardioprotective effects of red wine. Exp Clin Cardol 12:5-10
  21. Deyhim F, Patil BS, Villarreal A, Lopez E, Garcia K, Rios R, Garcia C, Gonzales C & Mandadi K (2007). Cranberry juice increases antioxidant status without affecting cholesterol homeostasis in orchidectomized rats. J Med Food 10:49-53 https://doi.org/10.1089/jmf.2006.218
  22. Dani C, Pasquali MA, Oliveira MR, Umezu FM, Salvador M, Henriques JA & Moreira JC (2008). Protective effects of purple grape juice on carbon tetrachloride-induced oxidative stress in brains of adults Wistar rats. J Med Food 11:55-61 https://doi.org/10.1089/jmf.2007.505
  23. Ruel G, Pomerleau S, Couture P, Lamarche B & Couillard C (2005). Change in plasma antioxidant capacity and oxidized low-density lipoprotein levels in men after short-term cranberry juice consumption. Metab clin exp 54:856-861 https://doi.org/10.1016/j.metabol.2005.01.031
  24. Cadenas E & Davies KJ (2000). Mitochondrial free radical generation, oxidative stress, and aging. Free Radic Biol Med 29:222-230 https://doi.org/10.1016/S0891-5849(00)00317-8
  25. Zhang K & Zuo Y (2004). GC-MS determination of flavonoids and phenolic and benzoic acids in human plasma after consumption of cranberry juice. J Agric Food Chem 52:222-227 https://doi.org/10.1021/jf035073r
  26. Bailey DT, Dalton C, Daugherty FJ & Tempesta MS (2007). Can a concentrated cranberry extract prevent recurrent urinary tract infections in women? A pilot study. Phytomedicine 14:237-241 https://doi.org/10.1016/j.phymed.2007.01.004
  27. Chen H, Zuo Y & Deng Y (2001). Separation and determination of flavonoids and other phenolic compounds in cranberry juice by high-performance liquid chromatography. J Chromatogr A 913:387-395 https://doi.org/10.1016/S0021-9673(00)01030-X
  28. Chu YF & Liu RH (2005). Cranberries inhibit LDL oxidation and induce LDL receptor expression in hepatocytes. Life Sci 77:1892-1901 https://doi.org/10.1016/j.lfs.2005.04.002
  29. Valentova K, Stejskal D, Bednar P, Vostalova J, Cihalik C, Vecerova R, Koukalova D, Kolar M, Reichenbach R, Sknouril L, Ulrichova J & Simanek V (2007). Biosafety, antioxidant status, and metabolites in urine after consumption of dried cranberry juice in healthy women: a pilot double-blind placebo-controlled trial. J Agric Food Chem 55:3217-3224 https://doi.org/10.1021/jf0636014
  30. Ghazala S, Aldoori WH, Davis PJ & Alkanani T (2008). Fortification of atherogenic diet with cranberry extract and spinach: plasma LDL oxidation and other atherosclerosis risk factors in rats. Atheroscler Suppl 9:11
  31. Vinson JA, Bose P, Proch J, A Kharrat H & Samman N (2008). Cranberries and cranberry products: powerful in vitro, ex vivo, and in vivo sources of antioxidants. J Agric Food Chem 56:5884-5891 https://doi.org/10.1021/jf073309b
  32. Celik H, Ozgen M, Serce S & Kaya C (2008). Phytochemical accumulation and antioxidant capacity at four maturity stages of cranberry fruit. Scientia Horticulturae 117:345-348 https://doi.org/10.1016/j.scienta.2008.05.005
  33. Sanchez-Moreno C, Larrauri JA & Saura-Calixto F (1999). Free radical scavenging capacity and inhibition of lipid oxidation of wines, grapes juices and related polyphenolic constituents. Food Res Intern 32:407-412 https://doi.org/10.1016/S0963-9969(99)00097-6
  34. American Institute of Nutrition (1977). Report of the American Institute of Nurtition Ad Hoc Committee on Standards for Nutritional Studies. J Nutr 107:1340-1348 https://doi.org/10.1093/jn/107.7.1340
  35. Benzie IF & Strain JJ (1996). The Ferric Reducing Ability of Plasma (FRAP) as a Measure of "Antioxidant Power": The FRAP Assay. Anal Biochem 239:70-76 https://doi.org/10.1006/abio.1996.0292
  36. Duthie SJ, Jenkinson AM, Crozier A, Mullen W, Pirie L, Kyle J, Yap LS, Christen P & Duthie GG (2006). The effects of cranberry juice consumption on antioxidant status and biomarkers relating to heart disease and cancer in healthy human volunteers. Eur J Nutr 45:113-122 https://doi.org/10.1007/s00394-005-0572-9
  37. Kontiokari T, Sundqvist K, Nuutinen M, Pokka T, Koskela M & Uhari M (2001). Randomised trial of cranberry-lingonberry juice and Lactobacillus GG drink for the prevention of urinary tract infections in women. BMJ 322:1571-1575 https://doi.org/10.1136/bmj.322.7302.1571
  38. Mckay DL & Blumberg JB (2007). Cranberries (Vaccinium macrocarpon) and cardiovascular disease risk factor. Nutr Rev 65:490-502 https://doi.org/10.1301/nr.2007.nov.490-502
  39. Sun J, Chu YF, Wu X & Liu RH (2002). Antioxidant and antiproliferative activities of common fruits. J Agric Food Chem 50:7449-7454 https://doi.org/10.1021/jf0207530