Comparison of anti-oxidant activities of seventy herbs that have been used in Korean traditional medicine

  • Ko, Seong-Hee (Major in Food and Nutrition, College of Human Ecology, Sookmyung Women's University) ;
  • Choi, Seong-Won (Department of Pharmacology, Seoul National University School of Medicine) ;
  • Ye, Sang-Kyu (Department of Pharmacology, Seoul National University School of Medicine) ;
  • Yoo, Sang-Ho (Department of Family Medicine, Hallym University Sacred Heart Hospital) ;
  • Kim, Hyun-Sook (Major in Food and Nutrition, College of Human Ecology, Sookmyung Women's University) ;
  • Chung, Myung-Hee (Department of Pharmacology, Seoul National University School of Medicine)
  • Published : 2008.09.30

Abstract

Many herbs have been used as therapeutics in Korean traditional medicine. In view of their clinical indications, anti-oxidant activity may contribute to their pharmacological effects. However, anti-oxidant information on these plants has not been available. In this study, seventy herbs which have been used in Korean traditional medicine were selected and screened for anti-oxidant activity using their water extracts. The anti-oxidant activity was assessed by their ability to inhibit three oxidation reactions; luminol/Fenton reagent, 2, 7-dichlorodihydrofluorescein(DCHF)/Fenton reagent and DCHF/peroxynitrite. In each assay, 70 herbs were divided into two groups; anti-oxidant group which inhibited the respective oxidation reaction and was majority(about 60 herbs), and pro-oxidant group which enhanced the oxidation reaction but was minority(more or less 10 herbs). When the herbs were listed in the order of their anti-oxidant strength, the orders obtained from each assay were found to be quite similar. The upper top rankers(more or less 10 herbs) in each assay showed strong activity compared to the others. The uppermost rankers in each assay were Rubus coreanus Miquel/Rubus schizostylus(覆盆子), Schisandra chinensis Baillon/Schizandra chinensis(五味子) and Terminalia chebula Retziusl Terminalia chebula(訶子). Of the pro-oxidant herbs, about 4-5 herbs were strongly pro-oxidant, which enhanced the control oxidation reactions to 150-300%. But the meaning of this observation is not known since few of them in one assay were also anti-oxidant in other assays. The results obtained in the present study may serve as information for understanding pharmacological effects of these herbs and developing new drugs from them.

Keywords

Anti-oxidants;herbs;chemiluminescence;peroxynitrite;Fenton reagent

References

  1. Eder K, Flader D, Hirche F & Brandsch C (2002). Excess dietary vitamin E lowers the activities of antioxidative enzymes in erythrocytes of rats fed salmon oil. Am J Clin Nutr 132:3400-3404
  2. Erdemoglu N, Kupeli E & Yesilada E (2003). Anti-inflammatory and antinociceptive activity assessment of plants used as remedy in Turkish folk medicine. J Ethnopharmacol 89:123-129 https://doi.org/10.1016/S0378-8741(03)00282-4
  3. Nakatani N (2000). Phenolic antioxidants from herbs and spices. Biofactors 13:141-146 https://doi.org/10.1002/biof.5520130123
  4. Nam JH, Jung HJ, Choi J, Lee KT & Park HJ (2006). The Antigastropathic Anti-rheumatic Effect of Niga-ichigoside F(1) and 23-Hydroxytormentic Acid Isolated from the Unripe Fruits of Rubus coreanus in a Rat Model. Biol Pharm Bull 29:967-970 https://doi.org/10.1248/bpb.29.967
  5. Siriwoharn T, Wrolstad RE, Finn CE & Pereira CB (2004). Influence of cultivar, maturity, and sampling on blackberry (Rubus L. Hybrids) anthocyanins, polyphenolics, and antioxidant properties. J Agric Food Chem 52:8021-8030 https://doi.org/10.1021/jf048619y
  6. Catapano AL, Maggi FM & Tragni E (2000). Low density lipoprotein oxidation, antioxidants, and atherosclerosis. Curr Opin Cardiol 15:355-363 https://doi.org/10.1097/00001573-200009000-00008
  7. Chang IS (2003). Treatise on Asian herbal Medicines Vol. 1, p.2. Natural Products Research Institute, Seoul National University publishing department, Seoul. Republic of Korea
  8. Jakubowski W & Bartoz G (2000). 2, 7-dichlorofluorescin oxidation and reactive oxygen species: What does it measure? Cell Biol Int 24:757-760 https://doi.org/10.1006/cbir.2000.0556
  9. Rani P & Khullar N (2004). Antimicrobial evaluation of some medicinal plants for their anti-enteric potential against multi-drug resistant Salmonella typhi. Phytother Res 18:670-673 https://doi.org/10.1002/ptr.1522
  10. Zhu YP (1998). Chinese Materia Medica chemistry, pharmacology and applications, p.235-245. Harwood academic publishers, Inc. Netherlands
  11. Bagchi D, Bagdhi M, Hassoun SJ & Stochs SJ (1995). In vitro and in vivo generation of reactive oxygen species, DNA damage and lactate dehydrogenase leakage by selected pesticides. Toxicology 104:129-140 https://doi.org/10.1016/0300-483X(95)03156-A
  12. Bushman BS, Phillips B, Isbell T, Ou B, Crane JM & Knapp SJ (2004). Chemical composition of craneberry (Rubus spp.) seeds and oils and their antioxidant potential. J Agric Food Chem 52:7982-7987 https://doi.org/10.1021/jf049149a
  13. Lee S, Suh I & Kim S (2000a). Protective effects of the green tea polyphenol (-)-epigallocatechin gallate against hippocampal neuronal damage after transient global ischemia in gerbils. Neurosci Lett 287:191-194 https://doi.org/10.1016/S0304-3940(00)01159-9
  14. Halliwell & Gutteridge (1984). Lipid peroxidation, oxygen radicals, cell damage, and antioxidant therapy. Lancet 1:1396-1397
  15. Hausladen A & Stamler JS (1999). Nitrosative stress. Meth Enzymol 300:389-395 https://doi.org/10.1016/S0076-6879(99)00143-3
  16. Libby P (2002). Inflammation in atherosclerosis. Nature 420:868-874 https://doi.org/10.1038/nature01323
  17. Lee YM, Kim H, Hong EK, Kang BH & Kim SJ (2000b). Water extract of 1:1 mixture of Phellodendron cortex and Aralia cortex has inhibitory effects on oxidative stress in kidney of diabetic rats. J Ethnopharmacol 73:429-436 https://doi.org/10.1016/S0378-8741(00)00302-0
  18. Naik GH, Priyadarsini KI, Bhagirathi RG, Mishra B & Mohan H (2005). In vitro antioxidant studies and free radical reactions of triphala, an ayurvedic formulation and its constituents. Phytother Res 19:582-586 https://doi.org/10.1002/ptr.1515
  19. Zanon FS, Ceriatti M, Rovera LJ, Sabini BA & Ramos T (1999). Search for antiviral activity of certain medicinal plants from Cordoba, Argentina. Rev Latinoam Microbiol 41:59-62
  20. Zheng W & Wang SY (2001). Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 49:5165-5170 https://doi.org/10.1021/jf010697n
  21. Gandhi NM & Nair CK (2005). Radiation protection by Terminalia chebula: some mechanistic aspects. Mol Cell Biochem 277:43-48 https://doi.org/10.1007/s11010-005-4819-9
  22. Monika B, Anurag P & Dhan P (2005). Phenolic contents and antioxidant activity of some food and medicinal plants. Int J Food Sci Nutr 56:287-291
  23. Wallace DC (1999). Mitochondrial diseases in man and mouse. Science 283:1482-1488 https://doi.org/10.1126/science.283.5407.1482
  24. Zhu H, Bannenberg G.L, Moldeus P & Shertzer HG (1994). Oxidation pathways for the intracellular probe 2, 7-dichlorofluorescein. Arch Toxicol 68:582-587 https://doi.org/10.1007/s002040050118
  25. Koteswara RN & Nammi S (2006). Antidiabetic and renoprotective effects of the chloroform extract of Terminalia chebula Retz. seeds in streptozotocin-induced diabetic rats. BMC Complement Altern Med 6:17-29 https://doi.org/10.1186/1472-6882-6-17
  26. Bent S & Ko R (2004). Commonly used herbal medicines in the United States: a review. Am J Clin Nutr 116:478-485
  27. Liu RH (2003). Protective role of phytochemicals in whole foods: implications for chronic disease prevention. Appl Biotechnol Food Sci Policy 1:39-46