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Effects of Feeding Fermented Colostrum Feed on the Growth to Piglets

발효초유사료 급여가 자돈의 성장에 미치는 영향

  • Na, Seuk-Han (Chung Mi Bio Inc.) ;
  • Choi, Seong-Hyun (Chung Mi Bio Inc.) ;
  • Renchinthand, Gereltuya (Division of Animal Science & Resources, College of Agriculture & Life Science, Chungnam National University) ;
  • Bae, Hyoung-Churl (Division of Animal Science & Resources, College of Agriculture & Life Science, Chungnam National University) ;
  • Nam, Myoung-Soo (Division of Animal Science & Resources, College of Agriculture & Life Science, Chungnam National University)
  • 나석한 (㈜청미바이오) ;
  • 최성현 (㈜청미바이오) ;
  • 랜친핸드 (충남대학교 농업생명과학대학 동물자원과학부) ;
  • 배형철 (충남대학교 농업생명과학대학 동물자원과학부) ;
  • 남명수 (충남대학교 농업생명과학대학 동물자원과학부)
  • Published : 2008.08.30

Abstract

This study was carried out to assess the fermentation properties of lactic acid bacteria (LAB) isolated from bovine colostrum and effects of feeding fermented colostrum feed on the growth to piglet. A total of 427 colonies were isolated from bovine colostrum on the BCP plate count agar. These LAB isolated were subcultured in 10% reconstituted skim milk, and seven strain thereof were selected for their highest acid productions, and two strain thereof were finally selected for their excellent sugar utilization. These strains were identified as Streptococcus thermophilus and Streptococcus macedonicus based on l6S rDNA sequencing data, named S. thermophilus CNB-11 and S. macedonicus CNB-11 respectively. For fermentation profiles, sugar utilization, acid production and viable cell counts were excellent in S. thermophilus CNB-11 as compared with S. macedoniclts CNB-11 after 48 hour. The effect of feeding fermented colostrum feed 0.5% using S. thermophilus CNB-11 was investigated for growth rate, analysis of blood and incidence of diarrhea. 24 heads of piglets were divided into two groups: the experimental and the control of 12 animals each. The average growth rate in the pigs fed fermented colostrum feed was higher 16.73% compared with control diet (p<0.05). There were no differences in the concentrations of blood glucose, cholesterol, albumin and globulin in pigs fed fermented colostrum feed as compared with control piglets. Incidence of diarrhea was no in pigs fed fermented colostrum feed as compared with control piglets.

Keywords

Streptococcus thermophilus CNB-11;Streptococcus macedonicus CNB-11;lactic acid bacteria;identification;growth rate;diarrhea

References

  1. Bae, H. C. and Nam, M. S. (2005) Fermentation properties of the mixed yogurt prepared with bovine milk and soybean milk. Korean J. Food Sci. Anim. Resour. 25, 483-493
  2. Bae, H. C., Min, J. Y., Kim, K. W., and Nam, M. S. (2008) Probiotic effects of fermented mixture of prepared with milk and soybean use to Lactobacillus salivarius sp. salivarius DF20 on piglets. Korea Patent 10-0803532
  3. Mistuoka, T. (1990) Bifidobacteria and their role in human health. J. Ind. Microbiol. 6, 263-268 https://doi.org/10.1007/BF01575871
  4. Nagao, F., Nakayama, M., Muto, T., and Okumura, K. (2000) Effects of a fermented milk drink containing Lactobacillus casei strain Shirota on the immune system in healthy human subjects. Biosci. Biotechnol. Biochem. 64, 2706-2708 https://doi.org/10.1271/bbb.64.2706
  5. Pollmann, D. S., Danielson, D. M., and Jr. Peo, E. R. (1980) Effect of Lactobacillus acidophilus on starter pigs fed a diet supplemented with lactose. J. Anim. Sci. 51, 638-644 https://doi.org/10.2527/jas1980.513638x
  6. Reiter. B. (1978) Review of the progress of dairy science: Antimicrobial systems in milk. J. Dairy Res. 45, 131-147 https://doi.org/10.1017/S0022029900016290
  7. Sandine, W. E., Muralidhara, K. S., Elliker, P. R., and England, D. E. (1972) Lactic acid bacteria in food and health: A review with special reference to enteropathogenic Escherichia coli as well as certain enteric disease and their treatment with antibiotics and lactobacilli. J. Milk Food Technol. 35, 691-702 https://doi.org/10.4315/0022-2747-35.12.691
  8. Lane, D. J. (1991) 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. In : Stackebrandt, E. and Goodfellow, M. (eds), John Wiley and Sons, Chichester, pp. 115-175
  9. Muralidhara, K. S., Sheggery, G. G., and Elliker, D. C. (1977) Effect of feeding lactobacillus on the coliform and lactobacillus flora of intestinal tissue and feces from piglets. J. Food Prot. 40, 288-295 https://doi.org/10.4315/0362-028X-40.5.288
  10. Shams, D. (1994) Growth factors in milk. Endocrine Regulations 28, 3-8
  11. Foley, J. A. and Otterby. D. E. (1978) Availability, storage, treatment, composition, and feeding value of surplus colostrum. A review. J. Dairy Sci. 61, 1033-1060 https://doi.org/10.3168/jds.S0022-0302(78)83686-8
  12. Larson, L. L., Owen, F. G., Albright, J. L., Appleman, R. D., Lamb, R. C., and Muller, L. D. (1977) Guidelines toward more uniformity in measuring and reporting calf experimental data. J. Dairy Sci. 60, 989-1003 https://doi.org/10.3168/jds.S0022-0302(77)83975-1
  13. Tacket, C. O., Binion, S. B., Bostwick, E., Losonsky, G., Roy, M. J., and Edelman, R. (1992) Efficacy of bovine immunoglobulin concentrate in preventing illness after Shigella flexneri challenge. Am. J. Trop. Med. Hyg. 47, 276-283 https://doi.org/10.4269/ajtmh.1992.47.276
  14. Donovan, S. M. and Odle, J. (1994) Growth factors in milk as mediators of infant development. Ann. Rev. Nutr. 14, 147-167 https://doi.org/10.1146/annurev.nu.14.070194.001051
  15. Besser, T. E. and Gay, C. C. (1994) The importance of colostrum to the health of the neonatal calf. Vet. Clin. Nor. Amer. - Food Anim. Pract. 10, 107-117 https://doi.org/10.1016/S0749-0720(15)30591-0
  16. Jeon, K. S. and Hwang, I. K. (2002) The hydrolysis of isoflavones by Bifidobacterium sp. Int-57 during soymilk fermentation. Korean Soybean Digest. 19, 42-47
  17. Johnson, J. L. (1994) Similarity analysis of rRNAs. In: Methods for general and molecular bacteriology. Gerhardt, P. R., Murray, G. E., Wood, W. A., and Krirg, N. R. (eds), American Society for Microbiology, Washington, DC, pp. 683-700
  18. Kim, S. K. and Hoe, K. C. (2000) Effects of preservative treatment, heating and freezing on compositional and microbiological changes of colostrum. J. Anim. Sci. Technol. 42, 659-668
  19. Aparna, H. S. and Salimath, P. V. (1999) Acidic glycoproteins of buffalo colostrum and their influence on the growth of Bifidobacterium bifidus. Nutr. Res. 19, 295-303 https://doi.org/10.1016/S0271-5317(98)00192-4
  20. Kato, I. K., Endo, K., and Yokokura, T. (1994) Effects of oral administration of Lactobacillus casei on antitumor responses induced by tumor resection in mice. Int. J. Immunopharmacol. 16, 29-34 https://doi.org/10.1016/0192-0561(94)90116-3
  21. Bae, H. C., Renchinthand, G., Na, S. H., Cho, S. H., and Nam, M. S. (2007) Studies on situation and utilization of domestic colostrum. Korean J. Food Sci. Ani. Resour. 27, 517-521 https://doi.org/10.5851/kosfa.2007.27.4.517
  22. Felsenstein, J. (1993) PHYLIP: Phylogenetic Inference Package, version 3.5. Seattle: University of Washington
  23. AOAC (1980) Official Methods of Analysis of the Association Official Analytical Chemists. Washington, DC
  24. Perdigon, G., Galdeano, C. M., and Valdez, J. C. (2002) Interaction of lactic acid bacteria with the gut immune system. Eur. J. Clin. Nutr. 56(suppl.), 21-26 https://doi.org/10.1038/sj.ejcn.1601278
  25. Thompson, J. D., Higgins, D. G., and Gibson, T. J. (1994) CLUSTALW: improving the sensitive of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acid Res. 22, 4673-4680 https://doi.org/10.1093/nar/22.22.4673
  26. Chang, Y. H., Kim, J. K., Kim, H. J., Kim, W. Y., Kim, Y. B., and Park, Y. H. (2000) Probiotic effects of Lactobacillus reuteri BSA-131 on piglets. Korean J. Appl. Microbiol. Biotechnol. 28, 8-13
  27. Kyriakis, S. C., Tsiloyiannis, V. K., Vlemmas, J., Sarris, K., Tsinas, A. C., Alexopoulos, C., and Jansegers, L. (1999) The effect of probiotics LPS 122 on the control of post-weaning diarrhoea syndrome of piglets. Res. Vet. Sci. 67, 223-228 https://doi.org/10.1053/rvsc.1999.0308
  28. Maeng, W. J., Kim, C. W., and Shin, H. T. (1989) Effect of feeding lactic acid bacteria concentrate (LBC, Streptococcus faecium cernelle 68) on the growth rate and prevention of scouring in piglet. Korean J. Anim. Sci. 31, 318-323

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