Changes in the Stability Properties of Methylcellulose Emulsions as Affected by Competitive Adsorption Between Methylcellulose and Tween 20

메칠셀룰로오스/Tween 20 경쟁 흡착이 메칠셀룰로오스 유화액의 안정도 특성에 미치는 영향

  • Hong, Soon-Taek (Division of Food and Culinary Science, Howon University)
  • 홍순택 (호원대학교 식품외식조리학부)
  • Published : 2008.10.31


The effect of Tween 20 addition on changes in the stability of methylcellulose (MC) emulsions (1 wt% MC, 10 wt% n-tetradecane, 20 mM bis-tris buffer, pH 7) was investigated by creaming stability and orthokinetic stability measurements. In the case of MC emulsions containing varying amounts of oil (1$\sim$30 wt%) and no Tween 20 added, creaming stability, judged by mean migration velocity of fat globules ($V_m$), was found to depend on droplet size: the larger the droplet size, the worse the stability [$V_m$: 0.326 $\mu$m $min^{-1}$ ($d_{32}$: 0.32 $\mu$m) ${\rightarrow}V_m$: 0.551 $\mu$m $min^{-1}$ ($d_{32}$: 0.53 $\mu$m)]. With Tween 20, creaming stability was found to be worse than the one without Tween 20, except for MC emulsion containing 0.2 wt% Tween 20. In addition, cream stability was the lowest with the lowest concentration of Tween 20 and a tendency to recover with increasing Tween 20 concentration [$V_m$: 0.598 $\mu$m $min^{-1}$ (0.01 wt%)${\rightarrow}V_m$: 0.389 $\mu$m $min^{-1}$ (0.2 wt%)] was found. From viscosity measurement for aqueous bulk phase of MC emulsions, such a change in the creaming stability was found to coincide well with the results of viscosity measurement. Therefore, it was reasonable to say that creaming stability of MC emulsions containing Tween 20 depended on MC concentration in aqueous bulk phase, which was in turn varied by competitive adsorption between MC and Tween 20 at the oil droplet surface. In case of orthokinetic stability, judged by destabilization time ($t_d$), it was found that the addition of Tween 20 resulted in lowered stability with more pronounce tendency at higher concentrations [$t_d$: 160 min (0.03 wt%)${\rightarrow}t_d$: 100 min (0.2 wt%)]. Moreover, combined with previous results, the orthokinetic stability of MC emulsions containing Tween 20 was found to be exponentially proportional to MC load. In conclusion, competitive adsorption between MC and Tween 20 may affect the stability properties of MC emulsion to varying extents, depending on the concentration of Tween 20.


  1. Shields M, Ellis R, Saunders BR. 2001. A creaming study of weakly flocculated and depletion flocculated oil-in-water emulsions. Colloid Surf A 178: 265-276
  2. Chanamai R, McClment DJ. 2000. Impact of weighting agents and sucrose on gravitational separation of beverage emulsions. J Agric Food Chem 48: 5561-5565
  3. Blijdenstein TBJ, van Vliet T, van der Linden E, van Aken GA. 2003. Suppression of depletion flocculation in oil-inwater emulsions: a kinetic effect of $\beta$-lactoglobulin. Food Hydrocoll 17: 661-669
  4. Durand A, Franks GV, Hosken RW. 2003. Particle sizes and stability of UHT bovine, cereal and grain milk. Food Hydrocoll 17: 671-678
  5. Sanchez CC, Rodriguez Patino JMR. 2005. Interfacial, foaming and emulsifying characteristics of sodium caseinate as influenced by protein concentration in solution. Food Hydrocoll 19: 407-416
  6. McClements DJ. 2007. Critical review of techniques and methodologies for characterization of emulsion stability. Crit Rev Food Sci Nutr 47: 611-649
  7. Dickinson E, Williams A. 1994. Orthokinetic coalescence of protein-stabilized emulsions. Colloid Surf A 88: 317-326
  8. Williams A. 1995. Orthokinetic stability and interfacial interactions in simple and multiple emulsions. PhD Dissertation. University of Leeds, Leeds. p 1-55
  9. Hong ST. 1998. Orthokinetic stability of $\beta#-lactoglobulinstabilized emulsions: effect of protein heat treatment and surfactant addition. J Korean Soc Food Sci Nutr 3: 133-142
  10. Zecher D, Gerrish T. 1997. Cellulose derivatives. In Thickening and Gelling Agents for Food. Imeson A, ed. Blackie Academic & Professional, London. p 60-87
  11. Sarker DK, Axelos M, Popineau Y. 1999. Methylcelluloseinduced stability changes in protein-based emulsions. Colloid Surf B 12: 147-160
  12. Hong ST. 2008. Influence of methylcellulose on properties of $\beta$-lactoglobulin emulsions. Food Eng Prog 12: 121-127
  13. Gullapalli RP, Sheth B. 1996. Effect of methylcellulose on the stability of oil-in-water emusions. Int J Pharm 140: 97-109
  14. Hong ST. 2008. Competitive displacement of methylcellulose from the oil-water interface by various emulsifiers. J Korean Soc Food Sci Nutr 37: 1271-1277
  15. McClements DJ. 2004. Food Emulsions: principles, practices, and techniques. 2nd ed. CRC Press, Boca Raton, Florida. p 461-513
  16. Kanzaki G, Berger EY. 1959. Colorimetric determination of methylcellulose with diphenylamine. Anal Chem 31: 1383-1385
  17. Lips A, Westbury T, Hart PM, Evans ID, Cambell IL. 1993. On the physics of shear-induced aggregation in concentrated food emulsions. In Food Colloids and Polymers: Stability and Mechanical Properties. Dickinson E, Walstra P, eds. Royal Society of Chemistry, Cambridge. p 31-42
  18. Holland FA. 1973. Fluid Flow for Chemical Engineers. Edward Arnold, London. p 1-38
  19. Sarkar N. 1984. Structural interpretation of the interafacial properties of aqueous solutions of methylcellulose and hydroxypropyl methylcellulose. Polym 25: 481-486
  20. McNamee BF, O'Riordan ED, O'Sullivan M. 1998. Encapsulation and microencapsulation properties of gum arabic. J Agric Food Chem 46: 4551-4555
  21. Dickinson E. 1992. An Introduction to Food Colloids. Oxford University Press, Oxford. p 79-122
  22. Dalgleish D, Srinivasan M, Singh H. 1995. Surface properties of oil-in-water emulsion droplets containing casein and tween 60. J Agric Food Chem 43: 2351-2355
  23. Sun B, Wei Y, Liu S, Zhang S. 2007. Oil-in-water emulsions stabilized by hydrophobically modified hydroxyethyl cellulose: adsorption and thickening effect. J Colloid Interface Sci 311: 228-236
  24. Goff HD, Jordan WK. 1989. Action of emulsifiers in promoting fat destabilization during the manufacture of ice cream. J Dairy Sci 72: 18-29
  25. van Dam B, Watts K, Campbell IJ, Lips A. 1995. On the stability of milk protein-stabilized concentrated oil-in-water food emulsions. In Food Macromolecules and Colloids. Dickinson E, Lorient D, eds. Royal Society of Chemistry, Cambridge. p 215-229
  26. Walstra P. 1993. Principles of emulsion formation. Chem Eng Sci 48: 333-349

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