Representative Parameter of Immunostimulatory Ginseng Polysaccharide to Predict Radioprotection

방사선 방어효과 예측 가능한 면역증강 인삼 다당체의 활성인자

  • Son, Hyeog-Jin (Laboratory of Radiation Cancer Sciences, Korea Institute of Radiological and Medical Sciences) ;
  • Shim, Ji-Young (Laboratory of Radiation Cancer Sciences, Korea Institute of Radiological and Medical Sciences) ;
  • Ahn, Ji-Yeon (Laboratory of Radiation Cancer Sciences, Korea Institute of Radiological and Medical Sciences) ;
  • Yun, Yeon-Sook (Laboratory of Radiation Cancer Sciences, Korea Institute of Radiological and Medical Sciences) ;
  • Song, Jie-Young (Laboratory of Radiation Cancer Sciences, Korea Institute of Radiological and Medical Sciences)
  • 손혁진 (한국원자력의학원 방사선 암응용연구팀) ;
  • 심지영 (한국원자력의학원 방사선 암응용연구팀) ;
  • 안지연 (한국원자력의학원 방사선 암응용연구팀) ;
  • 윤연숙 (한국원자력의학원 방사선 암응용연구팀) ;
  • 송지영 (한국원자력의학원 방사선 암응용연구팀)
  • Published : 2008.09.30

Abstract

According to the increase in the use of radiotherapy to cancer patients, many approaches have been tried to develop new agents for the protection of surrounding normal tissues. However, it is still few applied in the clinic as a radioprotector. We aim to find a representative parameter for radioprotection to easily predict the activity of in vivo experiment from the results of in vitro screening. The polysaccharide extracted from Panax ginseng was used in this study because the immunostimulator has been regarded as one of the radioprotective agent category and was already reported having a promising radioprotective activity through the increase of hematopoietic cells and the production of several cytokines. Mitogenic activity, AK cells activity and nitric oxide production were monitored for the in vitro immunological assay, and endogenous colony-forming unit (e-CFU) was measured as in vivo radioprotective parameter. The immunological activity was increased by the galactose contents of ginseng polysaccharide dependently. The result of this study suggests that mitogenic activity of splenocytes demonstrated a good correlation with in vivo radioprotective effect, and may be used as a representative parameter to screen the candidates for radioprotector.

References

  1. Russell NS, Bartelink H. Radiotherapy: The last 25 years. Cancer Treat Rev. 1999 Dec;25(6):365-376 https://doi.org/10.1053/ctrv.1999.0141
  2. Koenig KL, Goans RE, Hatchett RJ, Mettler FA, Schumacher TA, Noji EK, Jarrett DG. Medical treatment of radiological casualties: Current concepts. Ann Emerg Med. 2005 Jun;45(6):643-652 https://doi.org/10.1016/j.annemergmed.2005.01.020
  3. Maurya DK, Salvi VP, Krishnan Nair CK. Radioprotection of normal tissues in tumor-bearing mice by troxerutin. J Radiat Res (Tokyo). 2004 Jun;45(2):221-228 https://doi.org/10.1269/jrr.45.221
  4. Littlefield LG, Joiner EE, Colyer SP, Sallam F, Frome EL. Concentration-dependent protection against X-ray-induced chromosome aberrations in human lymphocytes by the aminothiol WR-1065. Radiat Res. 1993 Jan;133(1):88-93 https://doi.org/10.2307/3578261
  5. Purdie JW. A comparative study of the radioprotective effects of cysteamine, WR-2721, and WR-1065 in cultured human cells. Radiat Res. 1979 Feb;77(2):303-311 https://doi.org/10.2307/3575142
  6. Weiss JF, Landauer MR. Protection against ionizing radiation by antioxidant nutrients and phytochemicals. Toxicology. 2003 Jul 15;189(1-2):1-20 https://doi.org/10.1016/S0300-483X(03)00149-5
  7. Milas L, Murray D, Brock WA, Meyn RE. Radioprotectors in tumor radiotherapy: Factors and settings determining therapeutic ratio. Pharmacol Ther. 1988;39(1-3):179-187 https://doi.org/10.1016/0163-7258(88)90059-9
  8. Maisin JR. Bacq and alexander award lecture--chemical radioprotection: Past, present, and future prospects. Int J Radiat Biol. 1998 Apr;73(4):443-450 https://doi.org/10.1080/095530098142284
  9. Neta R. Role of cytokines in radioprotection. Pharmacol Ther. 1988;39(1-3):261-266 https://doi.org/10.1016/0163-7258(88)90070-8
  10. Neta R. Modulation with cytokines of radiation injury: Suggested mechanisms of action. Environ Health Perspect. 1997;105 Suppl 6:1463-1465 https://doi.org/10.2307/3433652
  11. Ainsworth EJ. From endotoxins to newer immunomodulators: Survival-promoting effects of microbial polysaccharide complexes in irradiated animals. Pharmacol Ther. 1988;39(1-3):223-241 https://doi.org/10.1016/0163-7258(88)90066-6
  12. Hanson WR, Houseman KA, Collins PW. Radiation protection in vivo by prostaglandins and related compounds of the arachidonic acid cascade. Pharmacol Ther. 1988;39(1-3): 347-356 https://doi.org/10.1016/0163-7258(88)90082-4
  13. Block KI, Mead MN. Immune system effects of echinacea, ginseng, and astragalus: A review. Integr Cancer Ther. 2003 Sep;2(3):247-267 https://doi.org/10.1177/1534735403256419
  14. Song JY, Han SK, Son EH, Pyo SN, Yun YS, Yi SY. Induction of secretory and tumoricidal activities in peritoneal macrophages by ginsan. Int Immunopharmacol. 2002 Jun;2(7):857-865 https://doi.org/10.1016/S1567-5769(01)00211-9
  15. Song JY, Han SK, Bae KG, Lim DS, Son SJ, Jung IS, Yi SY, Yun YS. Radioprotective effects of ginsan, an immunomodulator. Radiat Res. 2003;159(6):768-774 https://doi.org/10.1667/0033-7587(2003)159[0768:REOGAI]2.0.CO;2
  16. Honda S, Akao E, Suzuki S, Okuda M, Kakehi K, Nakamura J. High-performance liquid chromatography of reducing carbohydrates as strongly ultraviolet-absorbing and electrochemically sensitive 1-phenyl-3-methyl-5-pyrazolone derivatives. Anal Biochem. 1989;180(2):351-357 https://doi.org/10.1016/0003-2697(89)90444-2
  17. Schepetkin IA, Quinn MT. Botanical polysaccharides: Macrophage immunomodulation and therapeutic potential. Int Immunopharmcol. 2006;6:317-333 https://doi.org/10.1016/j.intimp.2005.10.005
  18. Kinnamon KE, Ketterling LL, Stampfli HF, Grenan MM. Mouse endogenous spleen counts as a means of screening for anti-radiation drugs. Proc Soc Exp Biol Med. 1980;164(3):370-373