The Effect of Bu-Zhong-Yi-Qi-Tang on Epidermal Melanocytes in Ultraviolet B-irradiated Mice

마우스에서 보중익기탕이 자외선 B 조사에 의한 표피멜라닌세포 변화에 미치는 영향

  • Lee, Hae-June (Korea Institute of Radiological & Medical Science) ;
  • Kim, Hwan-Sung (College of Veterinary Medicine, Chonnam National University) ;
  • Park, Young-Jong (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Joong-Sun (College of Veterinary Medicine, Chonnam National University) ;
  • Moon, Chang-Jong (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Jong-Choon (College of Veterinary Medicine, Chonnam National University) ;
  • Bae, Chun-Sik (College of Veterinary Medicine, Chonnam National University) ;
  • Jo, Sung-Kee (Advanced Radiation Technology Institute, KAERI) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University)
  • Published : 2008.09.30

Abstract

We induced the activation of melanocytes in the epidermis of C57BL/6 mice by ultraviolet B (UVB) irradiation and observed the effect of Bu-Zhong-Yi-Qi-Tang (BZYQT) on the formation, and decrease of UVB-induced epidermal melanocytes. C57BL/6 mice were irradiated by UVB $80\;mJ/cm^2$ (0.5 mW/sec) daily for 7 days, and BZYQT was intraperitoneally or topically applied pre- or post-irradiation. For the estimation of change of epidermal melanocytes, light microscopic observation with dihydroxyphenylalanine (DOPA) stain was performed. Split epidermal sheets prepared from the ear of untreated mice exhibited 11-16 melanocytes/$mm^2$, and one week after UV irradiation, the applied areas show an increased number of strongly DOPA-positive melanocytes with stout dendrites. But intraperitoneal or topical treatment with BZYQT before each irradiation interrupted UVB-induced pigmentation and resulted in a marked reduction in the number of epidermal melanocytes as compared to radiation control skin. The number and size of DOPA-positive epidermal melanocytes were also significantly decreased in intraperitoneally injected or topically applicated group after irradiation with BZYQT at 3rd and 6th weeks after irradiation. The present study suggests the BZYQT as inhibitor of UVB-induced pigmentation and depigmenting agent.

References

  1. 한약위원회, 조제지침연구소위원회. 한약조제 지침서 해설, 사단법인 대한약사회. 1995
  2. Cho JM, Sato N, Kikuchi K. Prophylactic anti-tumor effect of Hochu-ekki-to (TJ41) by enhancing natural killer cell activity. In Vivo. 1991;5:389-391
  3. Harada M, Seta K, Ito O, Tamada K, Li T, Terao H, Takenoyama M, Kimura G, Nomoto K. Concomitant immunity against tumor development is enhanced by the oral administration of a kampo medicine, Hochu-ekki-to (TJ-41: Bu-Zhong-Yi-Qi-Tang). Immunopharmacol Immunotoxicol. 1995;17:687-703 https://doi.org/10.3109/08923979509037189
  4. Kao ST, Yang SL, Hsieh CC, Yang MD, Wang TF, Lin JG. Immunomodulation of Bu-Zhong-Yi-Qi-Tang on in vitro granulocyte colony-stimulating-factor and tumor necrosis factor-alpha production by peripheral blood mononuclear cells. Immunopharmacol Immunotoxicol. 2000;22:711-720 https://doi.org/10.3109/08923970009016434
  5. Kao ST, Yeh CC, Hsieh CC, Yang MD, Lee MR, Liu HS, Lin JG. The Chinese medicine Bu-Zhong-Yi-Qi-Tang inhibited proliferation of hepatoma cell lines by inducing apoptosis via G0/G1 arrest. Life Sci. 2001;69:1485-1496 https://doi.org/10.1016/S0024-3205(01)01226-7
  6. Kuroiwa A, Liou S, Yan H, Eshita A, Naitoh S, Nagayama A. Effect of a traditional Japanese herbal medicine, Hochuekki-to (Bu-Zhong-Yi-Qi Tang), on immunity in elderly persons. Int Immunopharmacol. 2004;4:317-324 https://doi.org/10.1016/j.intimp.2003.12.004
  7. Furuya Y, Akashi T, Fuse H. Effect of Bu-zhong-yi-qi-tang on seminal plasma cytokine levels in patients with idiopathic male infertility. Arch Androl. 2004;50:11-14 https://doi.org/10.1080/01485010490250515
  8. Kobayashi H, Mizuno N, Kutsuna H, Teramae H, Ueoku S, Onoyama J, Yamanaka K, Fujita N, Ishii M. Hochu-ekki-to suppresses development of dermatitis and elevation of serum IgE level in NC/Nga mice. Drugs Exp Clin Res. 2003;29:81-84
  9. Shih HC, Chang KH, Chen FL, Chen CM, Chen SC, Lin YT, Shibuya A. Anti-aging effects of the traditional Chinese medicine bu-zhong-yi-qi-tang in mice. Am J Chin Med. 2000;28:77-86 https://doi.org/10.1142/S0192415X00000106
  10. Song QH, Kobayashi T, Hosoi T, Cyong JC. Effects of traditional Chinese medicines on murine bone metabolism in a microgravity environment. Am J Chin Med. 2003;31:739-749 https://doi.org/10.1142/S0192415X03001363
  11. Kim SH, Lee SE, Oh H, Kim SR, Yee ST, Yu YB, Byun MW, Jo SK. The radioprotective effects of bu-zhong-yi-qitang: a prescription of traditional Chinese medicine. Am J Chin Med. 2002;30:127-137 https://doi.org/10.1142/S0192415X02000144
  12. Laskin JD, Piccinini LA. Tyrosinase isozyme heterogeneity in differentiating B16/C3 melanoma. J Biol Chem. 1986;261:16626-16635
  13. Naeyaert JM, Eller M, Gordon PR, Park HY, Gilchrest BA. Pigment content of cultured human melanocytes does not correlate with tyrosinase message level. Br J Dermatol. 1991;125:297-303 https://doi.org/10.1111/j.1365-2133.1991.tb14161.x
  14. Maeda K, Fukada M. In vivo effectiveness of several whitening cosmetic components in human melanocytes. J Soc Cosmet Chem. 1991;42:361-368
  15. 이충환, 고영희. 멜라닌 생합성 저해물질의 탐색. 생물산업 1996;9:32-35
  16. Dawley RM, Flurkey WH. 4-hexylresocinol, a potent inhibitor of mushroom tyrosinase. J Food Sci. 1993;58:609-610 https://doi.org/10.1111/j.1365-2621.1993.tb04336.x
  17. Tomita K, Oda N, Ohbayashi M, Kamei H, Miyaki T, Oki T. A new screening method for melanin biosynthesis inhibitors using Streptomyces bikiniensis. J Antibiot (Tokyo). 1990;43:1601-1605 https://doi.org/10.7164/antibiotics.43.1601
  18. 김영근, 박윤기, 김홍직. 자외선B의 조사가 C57BL마우스 표피의 멜라닌세포에 미치는 영향. 대한피부과학회지 1988;26(2):139-144
  19. Nerya O, Vaya J, Musa R, Izrael S, Ben-Arie R, Tamir S. Glabrene and isoliquiritigenin as tyrosinase inhibitors from licorice roots. J Agric Food Chem. 2003;51:1201-1207 https://doi.org/10.1021/jf020935u
  20. Kahn V. Effect of kojic acid on the oxidation of DL-DOPA, norepinephrine, and dopamine by mushroom tyrosinase. Pigment Cell Res. 1995;8:234-240 https://doi.org/10.1111/j.1600-0749.1995.tb00669.x
  21. Chakraborty AK, Funasaka Y, Komoto M, Ichihashi M. Effect of arbutin on melanogenic proteins in human melanocytes. Pigment Cell Res. 1998;11:206-212 https://doi.org/10.1111/j.1600-0749.1998.tb00731.x
  22. Shin NH, Ryu SY, Choi EJ, Kang SH, Chang IM, Min KR, Kim Y. Oxyresveratrol as the potent inhibitor on dopa oxidase activity of mushroom tyrosinase. Biochem Biophys Res Commun. 1998;243:801-803 https://doi.org/10.1006/bbrc.1998.8169
  23. 김정아, 최지영, 손애랑, 박성희, 허광화, 이종구, 오인석, 김진준, 장현욱, 정시령, 장태수, 이승호. 대극과 식물로부터 분리한 천연폴리페놀의 멜라닌 생성 억제효과. 생약학회지 2004;35:157-163
  24. 김청택, 김원찬, 진무현, 김호정, 강상진, 강세훈, 정민환, 임영희. 전호의 멜라닌 생성억제 물질. 생약학회지 2002;33:395-398
  25. 최두영, 안소영, 이승기, 한정선, 김은철, 이향복, 신정현, 김은기, 노경호. 홍경천에 포함된 미백성분의 분리 및 성능검사. 한국생물공학회지 2004;19:169-173
  26. 천현자, 황상구, 이진선, 백승화, 전병훈, 우원홍. 소목의 부탄올 추출물에 의한 Melan-a 세포의 멜라닌생성 억제효과. 생약학회지 2002;33:130-136
  27. 이해준, 채세림, 김성호. 자외선 B를 조사한 마우스 표피멜라닌세포 변화에 대한 분죽(Phyllostachys nigra var. henenis Strapf)잎 추출물의 효과. Journal of Radiation Protection. 2007;32(2):59-64
  28. 김유찬, 윤재일. 자외선을 조사한 C57BL mice에서 자외선 조사부 및 차단부 표피내 멜라닌 세포의 변화에 관한 연구. 대한피부과학회지 1988;26:283-291
  29. Slominski A, Tobin DJ, Shibahara S, Wortsman J. Melanin pigmentation in mammalian skin and its hormonal regulation. Physiol Rev. 2004;84:1155-1228 https://doi.org/10.1152/physrev.00044.2003
  30. Gerson DE, Szabo G. Effect of single gene substitution on the melanocyte system of the C57BL mouse: quantitative and qualitative histology. Nature 1968;218:381-382 https://doi.org/10.1038/218381a0
  31. Reynolds J. The epidermal melanocytes of mice. J. Anat. 1954;88:45-58
  32. Jimbow K, Uesugi T. New melanogenesis and photobiological processes in activation and proliferation of precursor melanocytes after UV-exposure: ultrastructural differentiation of precursor melanocytes from Langerhans cells. J Invest Dermatol. 1982;78:108-115 https://doi.org/10.1111/1523-1747.ep12505758
  33. Yamakoshi J, Otsuka F, Sano A, Tokutake S, Saito M, Kikuchi M, Kubota Y. Lightening effect on ultravioletinduced pigmentation of guinea pig skin byoral administration of a proanthocyanidin-rich extract from grape seeds. Pigment Cell Res. 2003;16(6):629-638 https://doi.org/10.1046/j.1600-0749.2003.00093.x
  34. Jafari M, Felgner JS, Bussel II, Hutchili T, Khodayari B, Rose MR, Vince-Cruz C, Mueller LD. Rhodiola: a promising antiaging Chinese herb. Rejuvenation Res. 2007;10(4):587-602 https://doi.org/10.1089/rej.2007.0560