Effect of Lycii Fructus Powder on Lipid Metabolism in 1% Cholesterol Fed Rats

구기자 분말 첨가 식이가 1% 고콜레스테롤 급여 흰쥐의 지질 대사에 미치는 영향

  • Han, Sung-Hee (Department of Food of Nutrition, Wonkwang Health Science College) ;
  • Park, Sung-Hyee (Department of Orinetal Health Care, Wonkwang Digital University)
  • 한성희 (원광보건대학 식품영양과) ;
  • 박성혜 (원광디지털대학교 한방건강학과)
  • Published : 2008.08.31

Abstract

This study examined the effects of 2% Lycii fructus powder (LFP) supplementation on lipid metabolism in rats. Male Sprague-Dawley rats were administered 1% cholesterol and 0.25% sodium cholate to induce hypercholesterolemia. Then, 40 rats were divided into four diet groups: a normal diet group (NC), high cholesterol diet group (HC), normal diet plus 2% Lycii fructus powder (NC-LFP) group, and high cholesterol diet plus 2% Lycii fructus powder (HC-LFP) group. The HC group presented higher growth rates and liver weights than NC and NC-LFP however, growth rates and liver weights in the 2% LFP administered groups gradually decreased. HC also showed increased serum triglyceride, total cholesterol, free cholesterol, and LDL-cholesterol levels and decreased atherogenic index values, HDL-cholesterol, and phospholipid levels, whereas LFP group showed decreased serum triglyceride, total cholesterol, free cholesterol, and LDL-cholesterol levels as compared to HC. There were no differences in serum triglyceride, phospholipid, LDL-cholesterol, HDL-cholesterol, and free cholesterol concentrations between the normal diet groups (NC and NC-LFP). The high cholesterol diet groups (HC and HC-LFP) had significant increases in serum alanine aminotransferase (ALT), asparate aminotransferase (AST), alkaline phosphatase (ALP), and lactate dehydrogenase (LDHase) activities. And the 2% LFP administered groups had lower hepatic concentrations of total cholesterol and triglycerides than the HC groups. Overall, the results suggest that Lycii fructus powder has hypochloesterolemic effects by reducing serum and liver cholesterol contents.

Keywords

Lycii fructus;1% high cholesterol diet;lipid profiles;serum cholesterol content;AST;ALT;LDHase;ALP

References

  1. Akiyoshi S, Takane F, Kunio K. 1982. Isolation of 1,2-dehydroalpha- cyperone and solavetivone from Lycium chinese. phytochemistry, 21:2986-2995 https://doi.org/10.1016/0031-9422(80)85085-0
  2. Bligh EG, Dyer JA. 1989. A rapid method of total lipid extraction and purfication. Can J Biochem Physiol, 37:911-917
  3. Chung HK, Choi CS, Yang EJ, Kang MH. 2004. The effect of Lycii fructus beer intake on serum lipid profiles and antioxidant activity in rats. Korean J Food Cuture, 19(1):52-60
  4. Gilliard SE, Nelson CR and Maxwell C. 1985. Assimilation of cholesterol by Lactobacillus acidophilus. Appl Environ Microbiol, 49:377-381
  5. Hagulund O, Loustarinen R, Wallin I. 1991. The effect of fish oil on triglyceride, cholsterol, fibrinogen and malodialdehyde in humans supplementes with vitamin. Eur J Nutr, 12:165-172
  6. Kang JA and Kang JS. 1997. Effects of garlic and onion pn plasma an liver cholesterol and triglyceride and platelet aggregation in rate basal or cholesterol supplemented diets. Korean J Nutr, 32:132-138
  7. Kim BW, Roh KS. 2000. Study on the activity of GOT and GPT in the hepatotoxic rat treated Lycium chinense Mill. Korean J Biomed Lab Sci, 6:187-192
  8. Nam SM, Han SS, Oh DH, Jung ME, Kang IJ and Chung CK. 2002. Effects of eucommia ulmoides olivon ethanol extract on lipid metabolism and antioxidant enzymes activities of rats fed high fat. J Korean Soc Food Sci Nutr, 31(5):796-801 https://doi.org/10.3746/jkfn.2002.31.5.796
  9. Park OJ. 1994. Plasma lipids and fecal extracton of lipids in rats fed a high fat diet, a high cholesterol diet or a low fat/high sucrose diet. Kor J Nutr, 27(8):785-794
  10. Park EJ. 2002. Dietary carbohydrates effects on lipogenesis and the relationship of lipogenesis to blood insulin and glucose concentration. Br J Nutr, 87:247-253 https://doi.org/10.1079/BJN2001514
  11. Sheo HJ, Jun SJ, Lee MY. 1986. Effects of Lycii fructus extract on experimentally induced damage and alloxan diabetes in rabbits. J Korean Soc Food Nutr, 15(3):136-143
  12. Youna WG, Kim NJ, Hong ND. 1994. Pharmacological effects of Lycium chinense. Kor J Pharmacon, 25:264-271
  13. Joo HK. 1988. Study on development of tea by utilizing Lycium chinense and cornus officinalis. Korean J Dietary culture, 3(3):377-387
  14. Park JS, Park JD, Lee BC, Choi KJ. 2000. Effects of extracts from various parts of Lycium chinense Mill. on proliferation of mouse spleen cells. Korean J Medical Crop Sci, 8(4):291-296
  15. Choi SH, Lee MH, Shin CS, Sung C, Oh MJ, Kim CJ. 1996. Effects of storage condition on the quality of the wine and yakju made by Lycium chinense miller. Agriculture chemistry and biotechnology, 39:338-342
  16. Jang JY, Lee MK. 1998. Effects of serum lipid metabolism in rats with diet induced cholesterol mia. J Korean Soc Food Sci Nutr, 27:1211-1216
  17. Baker HJ, Lindsey JR, Weisbroth SH. 1984. The laboratory rat. Academic press Inc. New York. pp 123-139
  18. Kang YH, Park YH, Ha TY, Moon KD. 1996. Effects of pine needle extracts on serum and liver lipid contents in rats fed high fat diet. J Korean Soc Food Nutr, 25(3): 367-373
  19. Lee BY, Kim HM, Kim CJ, Park MH. 1992. Rheological properties of hot water extractable concentration of boxthorn(Lycii fructus) and mixed boxthorn. Korean J Food Sci Technol, 24(4):597-602
  20. Yun YP, Kang WS, Lee MY. 1996. The antithrombotic effects of green tea catechins. J Food Hyg Safety, 11:77-82
  21. Ki HY, Song SW, Ha CS, Han SS. 1993. Effects of the population density on growth and various physiological values of Sprague-Dawely rats. Korean Jab Ani Sci, 9:71-82
  22. Sohn IS, 1975. Studies on the hypercholesterolemia of koreans. Kor J Med, 18:354-357
  23. You SY. 1988. Medicinal plant culture. Ohsung Press Co. Seoul. pp 244-253
  24. Rosenfeld L. 1989. Lipoprotein analysis. Early methods in the diagnosis of atherosclerosis. Arch Pathol Lab Med, 113:1101-1110
  25. Cha JY, Cho YS, Yanagita T. 1999. Effects of cholesterol on heaptic phospholipid metabolism in rats fed a diet containing fish oil and beef tallow. J Food Sci Nutr, 4:125-129
  26. Lee BC, Park JS, Kwak TS, Moon CS. 1998. Variation of chemical properties in collected boxthorn varieties. Korean J Breed, 30:267-272
  27. Yao T, Sato M, Kobay Y, Wasa T. 1985. A perometric assays of total and free cholesterol in serum by the combined use of immobilized cholesterol esterases and cholesterol oxidant reactors and peroxidase electrode in a flow injection system. Anal biochem 149:387-397 https://doi.org/10.1016/0003-2697(85)90587-1
  28. Kim HS, Park YS, Kim CI. 1998. Changes of serum lipid profiles after eating Lycii fructus in rats fed high fat diet. J Korean Soc Food Nutr, 31(3):263-270
  29. Kim NJ, Youn WG, Hong ND. 1994. Pharmacological effects of Lycium chinense. Kor J Pharmacogn, 25:264-271
  30. Levy RI. 1981. Cholesterol lipoprotein, apoproteins and heart disease; present status and future prospects. Clin Chem, 27:653-662
  31. Sung IS, Kim MJ, Cho SY. 1997. Effect of quercus acutissima carruthers extracts on the lipid metabolism. J Kor Soc Food Sci Nutr, 26(2):327-333
  32. Kim HP, Lee EJ, Kim YC, Kim J, Kim HK, Park JH, Kim SY, Kim YC. 2002. Zeaxanthine dipalmitate from Lycium chinenese fruit reduces experimentally induced hepatic fibrosis in rats. Biolgical & pharmaceutical bulletin, 25:390-392 https://doi.org/10.1248/bpb.25.390
  33. Moon SJ. 1996. Nutrition problems of korean. Korean J Nutr, 29:371-380
  34. Do JR, Kim SB, Park YH, Kim DS. 1993. Angiotensin-I converting enzyme inhibitory activity by component of traditional the material. Korean J Food Sci Technol, 25(3):456-460
  35. Kang SM, Shim JY, Hwang SJ, Hong SG, Jang HE, Park MH. 2003. Effects of saengshik supplementation on health improvement in diet-induced hyperchole-sterolemic rats. J Korean Soc Food Sci Nutr, 32:906-912 https://doi.org/10.3746/jkfn.2003.32.6.906
  36. Oh SL, Kim SS, Min BY, Chung DH. 1990. Composition of free sugars, free amino acids, non-volatile organic acids and tannins in the extracts of L chinensis, A acutilobak, S chinensis B and A sessilflorum S. Korean J Food Sci Technol, 22:76-81
  37. Yun JS, Chung BH, Kim NY, Seong NS, Lee HY, Kim JD. 2003. Screening of 94 plant species showing ACE inhibitory activity. Korean J Medicinal Crop Sci, 11:246-251
  38. Korea national statistical office. 2008. The cause of death statistics 2006. Annual report on the cause of death statics. pp 20-30
  39. Wooller LA, Spady DK and Dietschy JM. 1992. Saturated and unsaturated fatty acids independently regulate low density lipoprotein receptor activity and production rate. J Lipid Res, 33:77-88
  40. Lee KA, Chung HY. 2000. Inhibitory effects of extracts from parmelia austrosinensis and P. prasorediosa on postprandial hyperglycemia. Korea J Food & Nutr, 13:134-138
  41. Shin JS, Kim KS, Jeong GH, Cheong CS. Cheong CS. 1997. Antidiabetic activity of Lycii fructs. Kor J Pharmacogn, 28:138-142
  42. Reeves PG, Nielsen FH, Fahey GC. 1993. AIN-93 Purified diets for laboratoary rodents final report of the American institute of nutrition and hoc writng committe on the reformulation of the AIN-76A rodent diet. J Nutr, 123:1939-1995 https://doi.org/10.1093/jn/123.11.1939
  43. Frantz ID, Dawson EA, Ashman DL, Gatewood LC, Barth GE. 1989. Test of the effects of lipid lowering by diet on cardiovascular risk. The Minnesota coronary survey. Atherosclerosis, 9:129-135
  44. Allred JB. 1990. Elevated blood cholesterol, A risk for heart disease that decreased with advancd age. J Am Diet Assoc, 90:574-576
  45. Oda T, Shikata T, Natio C, Suzuki H and Kanetaka T. 1970. Phospholipid fatty liver a report of three cases with a new type of fatty liver. Japn J Exp Med, 40:127-140
  46. Plaa GL, Charonneau M. 1994. Detection and evaluation of chemically induced liver injury. In principles and methods of toxicology. Hayes At Raven Press New York, 839-870