Changes in the Content of Individual Phenolic Compounds in Apple Slices during Cold Storage

냉장저장 중 사과슬라이스의 개별페놀성분함량과 제변화

  • Ahn, Sun-Choung (Department of Hotel & Restaurant Management, Shinheung College)
  • 안선정 (신흥대학 호텔외식경영과)
  • Published : 2008.08.31


The objectives of this study were to provide fundamental information on how individual phenolic compounds form on the inside of apple slices during cold storage, the changes in the content of four types of phenols, ingredient variation of individual phenolic compounds and the influence of phenolic compounds on enzymatic browning. This study measured the changes in the content of soluble solids, pH and vitamin C in order to investigate the correlations between these variables. HD and FA were the main phenolic compounds found in the apple slices, and HD was the most prevalent phenol. Furthermore, comparison of the CG and EP content revealed that there were more CGs than EPs. The phenol content tended to decrease considerably in the fresh apple slices and water-dipped apple slices but only slightly in the CP from sucrose-dipped apple slices and 0.5% ascorbic acid solution-dipped apple slices. The degree of browning increased in the following order: fresh apple slices, water-dipped apple slices, 0.5% ascorbic acid solution-dipped apple slices and CP from sucrose-dipped apple slices. The vitamin C content tended to decrease in the fresh apple slices, water-dipped apple slices, 0.5% ascorbic acid solution-dipped apple slices and CP from sucrose-dipped apple slices. The pH tended to increase in all sample groups, but the pH of the water-dipped apple slices was lower than that of the comparison group. The CP from sucrose-dipped apple slices had the lowest value of pH. The change in soluble solids tended to increase in all treatment groups, but this increase was less in the CP from sucrose-dipped apple slice. Correlation analysis revealed a high degree of correlation between browning and chlorogenic acid content. The results of the present study show that, when stored in the fridge, the change in phenol ingredient content in apple slices influences the browning of the slices. The results also showed that HD and FA were the main phenolic compounds, while CG was shown to have the greatest influence on browning.


individual phenolic compound;browning degree;apple slice;caramelization products (CP);antibrowning


  1. Burda, S. Oleszek, W. and Lee, C.Y. 1990. Phenolic compounds and their changes in apple during maturation and cold storage. J. Agric. Food Chem., 38(4):945-948
  2. Goupy, P., Amiot. M.L., Richard-forget, F., Duprat, F., Aubert, S. and Nicolas, J. 1995. Enzymatic Browning of model solutions and apple phenolic extracts by apple polyphenoloxidase. J. Food Science, 60(3):497-501
  3. Janovitz-klapp, A.H., Richard, F.C., Goupy, P.M. and Nicolas, J.J. 1990b. Kinetics studies on apple polyphenoloxidase, J. Agric., Food Chem, 38(7):1427-1441
  4. Murata, M., Tsurutani, M., Tomita, M., Homma, S. and Kaneko, K. 1995 Relationship between apple ripening and browning: Changes in polyphenol content and polyphenol oxidase, J. Agric. Food Chem., 43(5):1115-1121
  5. Peleg, H., Naim, M., Rouself, R.L. and Zehavi, U. 1991. Distribution of bound and free phenolic acids in oranges (citrus sinesis) and grapefruits (citrus paradisi), J. Sci. Food. Agric., 57:417-426
  6. Pilarcano, M., Becgono de Ancos, and Lobo, G. 1995. Peroxidase and polyphenoloxidase activities in Papaya during postharvest ripening and after freezing/thawing, J. Food Sci., 60(4):815-820
  7. Sapers, G.M., El-atawy, Y.S., Hicks, K.B. and Garzarella, L. 1989. Effect of emulsifying agents on inhibition of enzymatic browning in apple juice by ascorbyl palmitate,laurate and decanoate. J. Food Sci., 54(4):1096-1097
  8. Spanos, G.A., Wrolstad, R.E. and Hentherbell, D.A. 1990. Influence of processing and storage on the phenolic composition of apple juice. J. Agric. Food Chem., 38:1572-1579
  9. Walker, J.R.L. 1969. Inhibition of the apple phenolase system through intection by penicillium expansion, phytochem., 8:561-566
  10. Weller, A., Sims, C.A., Matthews, R.F., Bates, R.P. and Brecht, J.K. 1997. Browning susceptibility and changes in composition during storage of carambola slices. J. Food Sci., 62(2):256-260
  11. Ahn, S.C. and Lee, G.C. 2005. Effects of antibrowning agents on browning of apple slices during cold storage. Korean J. Food Cookery Sci., 21(1):24-32
  12. Amiot, M.J., Tacchini, M., Auburt, S., and Nicolas, J. 1992. Phenolic composition and browning susceptibility of various apple cultivars at maturity. J. Food Sci., 57(4):958-962
  13. Dijkstra, L. and Walker, J.R.L. 1992. Enzymatic browning in Apricots, J. Sci. Food Agric., 58:41-48
  14. Mosel, H.O. and Herman, K. 1974. Changes in catechins and hydroxycinnamic acid derivatives during development of apples and pears, J. Sci. Food Agric, 25:251-256
  15. Dao, L. and Friedman, M. 1992. Chlorogenic acid content of fresh and processed potatoes determined by ultraviolet spectrophotometry, J. Agric. Food Chem., 40:2152-2156
  16. Mondy, N.Y., Geddle-Dahl, S.B. and Mobley, E.O. 1966 Relationship of specific gravity to the enzyme activity and phenolase content of potatoes, J. Food Sci., 31:157-160
  17. Porter, O.C. Pharr, D.H., kushman, L.J., Pop, D.T. 1976. Discoloration of chilled sweet potato (Ipomoea hatatas(L.) Lac.) roots: Reaction related to cultivar differences, J. Food Sci., 91:938-941
  18. 황혜정, 김성수, 윤광호. 사과주스의 제조과정 중 페놀계물질 의 변화, PA077, 식품과학회 논문초록집, 1997
  19. Lee, C.Y., Kaghn, V., Jaworski, A.W. and Brown, S.K. 1990. Enzymatic browning in relation to phenolic compounds and polyphenol oxidase activity among various peach cultivars, J. Agric. Food Chem., 38:99-101
  20. Seib, P.A., and Lino, M.L. 1987. Ascarbiate-2-polyphosbuate esters and method of making same., U.S. Patent., 4:647, 672
  21. Oszmianski, J. and Lee, C.Y. 1990. Inhibition of polyphenol oxidase activity and browning by honey. J. Agric. Food Chem., 38:1892-1895
  22. Kim, D.M., Smith, N.L. and Lee, C.Y. 1993. Apple cultivar variation in response to heat treatment and minimal processing, J. Food Sci, 58(5):1111-1124
  23. Weaver, C. and Charley, H. 1974. Enzymatic browning of ripening bannas. J. Food Sci., 39:1200-1202
  24. Cheynier, V.F., Osse, C., and Rigaud, J. 1989. Oxidation of grape juice phenolic compounds in model solution, J. Food Sci, 53:1729-1734
  25. Cheynier, V., Basson, N., and Rigaud, J. 1988. Mechanism of transcaffeoyl- tartaric acid and catechic oxidation in model solutions containg grape polyphenol oxidase, J. Agric. Food. Chem, 37:1069-1971
  26. Walker, J.R.L., Wilson, E.L. 1975. Studies on the enzymatic browning of apples, Inhibition of apple o-dephenoloxidase by phenolic acids, J. Sci. Food Agric. 26:1825-1831
  27. Amiot, M.J., Tacchini, M., Aubert, S.Y. and Oleszek, W. 1995. Influence of cultivar,maturity stage and storage conditions on phenolic composition and enzymatic browning of pear fruits, J. Agric. Food Chem., 43:1132-1137
  28. Coseteng, M.Y. and Lee, C.Y. 1987. Changes in apple polyphenoloxidase and polyphenol concentrations in relation to degree of browning, J. Food Science, 52(4):985-989
  29. Harel, E., Mayer, A.M. and Sham, Y. 1966. Catechol oxidases endogeneous substrates and browning in developing apples, J. Sci. Food Agric. 17(9):389-391
  30. Janovitz-Klapp, A.H., Richard, F.C, Goupy, P.M., and Nicolas, J.J. 1990a. Inhibition studies on apple polyphenol oxidase. J. Agric. Food Chem., 38:926-931