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The Antidermatophytic Potential of the Marine Isolate of Aspergillus sp. Collected from South Coast of Korea

  • Bajpai, Vivek K. ;
  • Kang, Sun-Chul
  • Published : 2008.03.31

Abstract

This study was carried out to assess the antidermatophytic potential of the ethyl acetate(EtOAc) extract of the marine isolate of Aspergillus sp.. The fungus was isolated by serial dilution, and was identified Aspergillus sp.. The EtOAc extract of the fungus was examined to evaluate the antidermatophytic efficacy against the fungal pathogens infecting human skin using the disc diffusion and MIC(minimum inhibitory concentration) determination methods. The EtOAc extract($5{\mu}l\;disc^{-1}$) was considered to have the antidermatophytic activity based on the inhibition percentage of the mycelial growth of the fungi tested such as Trichophyton mentagrophytes KCTC 6085, Microsporum canis KCTC 6591, Microsporum canis KCTC 6348, Trichophyton rubrum KCTC 6352, Microsporum canis KCTC 6349 and Trichophyton mentagrophytes KCTC 6316. The percentage of the inhibition ranged from 54% to 81, and the MIC obtained was 62.5, 62.5, 250, 125, 125, and $125{\mu}g\;ml^{-1}$, respectively. The extract had a strong detrimental effect on the spore germination of the tested skin infectious pathogens. These findings strongly support the role of the ethyl acetate extract as a potential antidermatophytic agent.

Keywords

marine fungus;Aspergillus sp.;dermatophytes;minimum inhibitory concentration;spore germination

References

  1. Baker, J. H., Goodpasture, H. C., Kuhns, H. R., and Rinaldi, M. G. (1989) Fungemia caused by an amphotericin B-resistant isolate of Sporothrix schenckii. Arch. Pathol. Lab. Med. 113, 1279-1281
  2. Willocks, L., Leen, C. L. S., Brettle, R. P., Urquart, D., Russel, T. B., and Milne, J. R. (1991) Fluconazole resistance in AIDS patients. J. Antimicrob. Chemother. 28, 937-939 https://doi.org/10.1093/jac/28.6.937
  3. Koenig, H. (1995) Guide de Mycologie MeA dicale. Ed. Ellipses: Paris
  4. Martinez, R. C. R., De Martinis, E. C. P. (2005) Antilisterial activity of a crude preparation of Lactobacillus sakei 1 bacteriocin and its lack of influence on Listeria monocytogenes haemolytic activity. Food Control 16, 429-433 https://doi.org/10.1016/j.foodcont.2004.05.002
  5. Fenical, W. (1993) Chemical studies of marine bacteria: developing a new resource. Chem. Rev. 93, 1673-1683 https://doi.org/10.1021/cr00021a001
  6. Grewe, M., Tsiotos, G. G., De-Leon, E. L., and Sarr, M. G. (1999) Fungal infection in acute necrotizing pancreatitis. J. Am. Coll. Surg. 188, 408-414 https://doi.org/10.1016/S1072-7515(98)00334-2
  7. Proksch, P., Edrada-Ebel, R., and Ebel, R. (2003) Drugs from the sea-opportunities and obstacles. Mar. Drugs 1, 5-17 https://doi.org/10.3390/md101005
  8. Faulkner, D. J. (2001) Marine natural products. Nat. Prod. Rep. 18, 1-49 https://doi.org/10.1039/b006897g
  9. http://www.botany.utoronto.ca/ResearchLabs/Mallo chLab/Malloch/Moulds/Isolation.html
  10. Collier, L., Balows, A., and Sussman, M. (1998) Topley & Wilson's Microbiology and Microbial Infections (9th edn), vol. 4. Arnold: London, Sydney, Auckland, New York
  11. Larone, D. H., 1995. Medically Important Fungi - A Guide to Identification (3rd edn), ASM Press: Washington DC
  12. Rana, B. K., Singh, U. P., and Taneja, V. (1997) Antifungal activity and kinetics of inhibition by essential oil isolated from leaves of Aegle marmelos. J. Ethanopharmacol. 57, 29-34 https://doi.org/10.1016/S0378-8741(97)00044-5
  13. St-Germain, G., and Summerbell, R. (1996) Identifying filamentous fungi-A Clinical Laboratory Handbook (1st edn), Star Publishing Company: Belmont, California
  14. Duru, M. E., Cakir, A., Kordali, S., Zengin, H., Harmandar, M., Izumi, S., and Hirata, T. (2003) Chemical composition and antifungal properties of essential oils of three Pistacia species. Fitoterapia 74, 170-176 https://doi.org/10.1016/S0367-326X(02)00318-0
  15. Murray, P. R., Baron, E. J., Pfaller, M. A., Tenover, F. C., and Yolke, R. H. (1995) Manual of Clinical Microbiology (6th edn), ASM: Washington DC
  16. Gadhi, C. A., Benharref, A., Jana, M., Basile, A. M., Contet-Audonneau, N., and Fortier, B. (2001) Antidermatophytic Properties of Extracts from the Leaves of Aristolochia paucinervis Pomel. Phytother. Res. 15, 79-81 https://doi.org/10.1002/1099-1573(200102)15:1<79::AID-PTR953>3.0.CO;2-K
  17. Liu, C. H., Liu, J. Y., Huang, L. L., Zou, W. X., and Tan, R. X. (2003b) Absolute configuration of keisslone, a new antimicrobial metabolite from Keissleriella sp. YS4108, a marine filamentous fungus. Planta Med. 69, 481-483 https://doi.org/10.1055/s-2003-39696