Characterization of Phosphate-solubilizing Microorganisms in Upland and Plastic Film House Soils

밭과 시설재배지 토양의 인산가용화 미생물의 특성

  • 서장선 (농촌진흥청 국립농업과학원) ;
  • 권장식 (농촌진흥청 국립농업과학원)
  • Received : 2008.09.19
  • Accepted : 2008.10.11
  • Published : 2008.10.30


With the aim to explore the possible role of phosphate-solubilizing bacteria in soil, we conducted a survey of phosphate-solubilizing microorganisms colonizing in upland and plastic film house soils. Soil EC, pH, organic matter, available phosphate, exchangeable cation such as potassium, calcium and magnesium, and total P of plastic film house soils were higher than those of upland soils. Phosphate-solubilizing bacteria population was higher in plastic film house soils than upland soils, but species of phosphate-solubilizing bacteria was more diverse in the upland soils than the plastic film house soils. There was significant positive correlation between phosphate solubilization and phosphate-solubilizing bacteria in soils. Bacillus, Cedecea, Brevibacillus, Paenibacillus, Pseudomonas, Serratia spp. were isolated from upland soils and Bacillus and Cellulomonas spp. were from plastic film house soils.


Upland soil;Plastic film house soil;Phosphate-solubilizing bacteria


  1. Omar, S.A. 1998. The role of rock-phosphate-solubilizing fungi and vesicular-arbuscular-mycorrhiza(VAM) in growth of wheat plants fertilized with rock phosphate. World J. Microbiol. Biotechnol. 14: 211-218.
  2. Salih, H.M., A.I. Yonka, A.M. Abdul-Rahem, and B.H. Munam, 1989. Availability of phosphorous in calcareous soil treated with rock phosphate or superphosphate as affected by phosphate dissolving fungi. Plant and Soil 120, 181.185.
  3. Vassilev, N., Vassilev, M., 2003. Biotechnological solubilization of rock phosphate on media containing agro-industrial wastes. Appl. Microbiol. Biotech. 61: 435-440.
  4. Wu, S.C., Z.H. Cao, Z.G. Li, K.C. Cheung, and M.H. Wong. 2005. Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. Geoderma. 125:155-166
  5. 농촌진흥청. 1988. 토양화학분석법. 삼미인쇄사
  6. 토양미생물연구회. 1992. 토양미생물실험법. 양현당.
  7. Vesquez, P., G. Holguin, M.E. Puente, A. Lopez-Cortes, and Y. Bashan, 2000. Phosphate-solubilizing microorganisms associated with the rhizosphere of mangroves in a semiarid coastal lagoon. Biol. Fertil. Soils. 30: 460-468.
  8. Rodriguez, H., and R. Fraga. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol. Adv. 17:319-339.
  9. Nautiyal C.S. 1999. An efficient microbiological growth medium for screening phosphate-solubilizing microorganisms. FEMS Microbiology Letters. 170: 265-270.
  10. Perez, E., M. Sulbaran, M.M. Ball, and L.A. Yarzabal. 2007. Isolation and characterization of mineral phosphate-solubilizing bacteria naturally colonizing a limonitic crust in the south-eastern Venezuelan region. Soil Biol. Biochem. 39: 2905-2914.
  11. Illmer, P, and F. Schinner. 1992. Solubilization of inorganic phosphates by microorganisms isolated from forest soil. Soil Biol. Biochem. 24: 389-395.
  12. Sing, S., and K.K. Kapoor. 1994. Solubilization of insoluble phosphates by bacteria isolated from different sources. Environ. Ecol. 12: 51-55.
  13. Suh, J. S., S. J. Kim, H. J. Noh, J. S. Kwon, and W. K. Jung, 2007, Long-term compositing and fertilization impact on dehydrogenase-producing bacteria and dehydrogenase activity in rice paddy soil. Korean J. Soil Sci. Fert. 40(4), 229-233.
  14. Mittala, V., O. Singha, H. Nayyarb, J. Kaura, and R. Tewaria. 2008. Stimulatory effect of phosphate- solubilizing fungal strains (Aspergillus awamori and Penicillium citrinum) on the yield of chickpea (Cicer arietinum L. cv. GPF2). Soil Biol. Biochem. 40: 718-727
  15. Son, H.J., G.T. Park, M.S. Cha, and M.S. Heo. 2006. Solubilization of insoluble inorganic phosphates by a novel salt- and pH-tolerant Pantoea agglomerans R-42 isolated from soybean rhizosphere. 97: 204-210.
  16. Asea, P.E.A., R.M.N. Kucey, J.W.B. Stewart, 1988. Inorganic phosphate solubilization by two Penicillium species in solution culture. Soil Biol. Biochem. 20: 459-464.