Isolation and Characteristics of Photosynthetic Bacterium, Erythrobacter longus SY-46 which Produces Bacterial Carotenoids

Bacterial Carotenoids를 생산하는 광합성세균 Erythrobacter longus SY-46의 분리 및 특성

  • Kim, Yun-Sook (Department of Microbiology, Pukyong National University) ;
  • Lee, Dae-Sung (Department of Microbiology, Pukyong National University) ;
  • Jeong, Seong-Yun (Korea Bio-IT Foundry Center, Pusan National University) ;
  • Lee, Won-Jae (Department of Microbiology, Pukyong National University)
  • 김윤숙 (부경대학교 미생물학과) ;
  • 이대성 (부경대학교 미생물학과) ;
  • 정성윤 (부산대학교 한국 Bio-IT 파운드리 센터) ;
  • 이원재 (부경대학교 미생물학과)
  • Published : 2008.04.30


The aerobic photosynthetic bacterium, which produces bacterial carotenoids was isolated and identified from coastal marine environments. This bacterium was identified by 16S rDNA sequencing and designated as Erythrobacter longus SY-46. E. longus SY-46 was Gram negative and rod shape, and the optimal culture conditions were $25^{\circ}C$, pH 7.0, and 3.0% NaCl concentration, respectively. The carbon and nitrogen sources required for the optimal growth were lactose and tryptone, respectively. Fatty acid compositions of E. longus SY-46 were $C_{18:1}$(78.32%), v-linolenic acid($C_{18:3n9.12.15c}:3.83%$), margaric acid($C_{17:0}$: 3.38%), palmitic acid($C_{16:0}$: 3.07%), and docosahexaenoic acid($C_{22:6n3}$: 2.21%). In addition, E. longus SY-46 showed the characteristic absorption peaks of bacterial carotenoids(in the region of 450 to 480 nm) and bacteriochlorophyll(770 to 772 nm). Major carotenoids of E. longus SY-46 were polyhydroxylated xanthophylls such as fucoxanthin and zeaxanthin.


Aerobic photosynthetic bacterium;Bacterial carotenoids;Bacteriochlorophyll;Erythrobacet longus SY-46


  1. Holt S. C., Marr A. G., 1965, Isolation and purification of the intracytoplasmic membranes of Rhodospirillum, J. Bacteriol., 89, 1413-1420
  2. Harashima K., Nakagawa M., Murata N.,. 1982, Photochemical activities of bacteriochlorophyll in aerobically grown cells of heterotrophs, Erythrobacter species(Och 114) and Erythrobacter longus(Och 101), Plant Cell Physiol., 23, 185-193
  3. 紫田李雄, 1965, 生體中における光合成色素, 蛋白質核酸醱酵 10, 1377-1385pp
  4. Harashima K., Nakada H., 1983, Carotenoid and ubiquinone in aerobically grown cells of an aerobic photosynthetic bacterium, Erythrobacter species Och114, Agric. Biol. Chem., 47, 1057-1063
  5. Shiba T., Simidu U., Taga N., 1979, Distribution of aerobic bacteria which contain bacteriochlorophyll a, Appl. Environ. Microbiol,, 38, 43-45
  6. Shiba T., Simidu U., 1982, Erythrobacter longus gen. nov., sp. nov., an aerobic bacterium which contains bacteriochlorophyll a, Int. J. Syst. Bacteriol., 32, 211-217
  7. Sato K., 1978, Bacteriochlorophyll formation by facultative methylotrophs, Protaminobacter ruber and Pseudomonas Am1, FEBS Lett., 85, 207-210
  8. Harashima K., Shiba T., Totsuka T., Simidu U., Taga N., 1978, Occurrence of bacteriochlorophyll a in a strain of and aerobic heterotrophic bacterium. Agric. Biol. Chem., 42, 1627-1628
  9. Raphael L., Cottrell M. T., Ras J., Ulloa O., Obernosterer I., Claustre H., Kirchman D. L., Lebaron P., 2007, High abundances of aerobic anoxygenic photosynthetic bacteria in th South Pacific Ocean, Appl. Environ. Microbiol,, 73, 4198-4205
  10. Lee W. J., Park Y. S., Park Y. T., Kim S. J., Kim K. Y., 1997, Studies on the availability of marine bacteria and the environmental factors for the mass culture of the high quality of rotifer and Artemia, J. Kor. Fish. Soc., 30(3), 319-328
  11. Shiba T., 1987, $O_2$ regulation of bacteriochlorophyll synthesis in the aerobic bacterium Erythrobacter, Plant Cell Physiol., 28, 1313-1320
  12. Bertram J. S., Vine A. L., 2005, Cancer prevention by retinoids and carotenoids: independent action on a common target, Biochim. Biophys. Acta, 1740, 170-178
  13. Kurihara H., Koda H., Asami S., Kiso Y., Tanaka T., 2002, Contribution of the antioxidative property of astaxanthin to its protective effect on the promotion of cancer metastasis in mice treated with restraint stress, Life Sci., 70, 2509-2520
  14. Chew B. P., Park J. S., 2004, Carotenoid action on the immune response, J. Nutr., 134, 257-261
  15. Matsuno T., 1994, Mechanism of carotenoid, carotenoid of marine of organisms, 1st ed., Kanshohen Kouseisha Kouseikaku, Tokyo, 9-22pp
  16. Macrae R., Robbison R. K., Sader M. J., 1992, Encyclopedia of food science and technology, 1st ed., Academic Press, London, 293pp
  17. Hoffmann J., Linseisen J., Riedl J., Wolfram G., 1999, Dietary fiber reduces the antioxidative effect of a carotenoid and alpha-tocopherol mixture on LDL oxidation ex vivo in humans, Eur. J. Nutr., 38(6), 278-285
  18. Farombi E. O., Britton G., 1999, Antioxidant activity of palm oil carotenes in peroxyl radical-mediated peroxidation peroxidation of phosphatidyl choline liposomes, Redox Rep., 4(1-2), 61-68
  19. Pfennig N., TrŪper H. G., 1984, Anoxygenic photosynthetic bacteria, In: Bergey's manual of systematic bacteriology, 1st ed., Staley J. T., Bryant M. P., Prennig N., Holt J. G., The Williams and Wilkins Co., Baltimore, 1635-1709pp
  20. Gerhardt P., Murray R. G., Costilow E. R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B., 1981, Manual of method for general bacteriology, 1st ed., Am. Soc. Microbiol., Washington D.C., 135-154pp
  21. Seong C. S., 1997, Identification of Microorganism Responsible for Anaerobic Degradation of Pentachlorophenol Using RFLP Analysis of PCR Amplified 16S rDNAs, Ph. D. Dissertation, Dept. of Biology, Inje University, Busan
  22. Moyer C. L., Dobbs F. C., Karl D. M., 1994, Estimation of diversity and community structure through RFLP distribution analysis of bacterial 16S rRNA genes from a microbial mat at an active, hydrothermal vent, Loihi Seamount, Hawaii, Appl. Environ. Microbiol., 60, 871-879
  23. Benson H. J., 1990, Microbiological applications; A laboratory manual in general microbiology, 5th ed., Wm. C. Brown Publishers, 40-134pp
  24. MacFaddin J. F., 1980, Biochemical tests for identification of medical bacteria, 2nd ed., Williams and Wilkins Co., Baltimore, 36-308pp
  25. Takaichi S., Shimada K., Ishidsu J., 1990, Carotenoid from the aerobic photosynthetic bacterium, Erythrobacter longus: ${\beta}-carotene$ and its hydroxyl derivatives, Arch. Microbiol., 153, 118-122
  26. Shiba T., 1991, Roseobacter litoralis gen. nov., sp. nov., and Roseobacter denitrificans sp. nov., aerobic pink-pigmented bacteria which contain bacteriochlorophyll a, Syst. Appl. Microbiol., 14, 140-145
  27. Ciegler A, 1965, Microbial carotenogenesis, Adv. Appl. Microbiol, 7, 1-34
  28. Sambrook J., Fritsch E. F., Maniatis T., 1989, Molecular cloning, a laboratory manual, 2nd ed., Cold Spring Harbor Laboratory, New York, 25-28pp
  29. Shioi Y., 1986, Growth characteristics and substrate specificity of aerobic photosynthetic bacterium,Erythrobacter sp.(Och114), Plant Cell Physiol., 27, 567-572
  30. Bligh E. G., Dyer W. J., 1959, A rapid method of total lipid extraction and purification, Can. J. Biochem. Physiol., 37, 911-917
  31. Pfennig N., TrŪper, H. G.. 1974, The photosynthetic bacteria, In: Bergey's manual of determinative bacteriology, 8th ed., Buchanan R. E., Gibbons N. E., Williams and Wilkins Co., Baltimore, 24-64pp
  32. Urakami T., Komagata K., 1988, Cellular fatty acid composition with special reference to the existence of hydroxy fatty acids, and occurrence of squalece and sterols in species of Rhodospirillaceae genera and Erythrobacter longus, J. Gen. Appl. Microbiol. 34, 67-84
  33. Show N., 1974, Lipid composition as a guide to the classification of bacteria, Adv. Appl. Microbiol., 17, 63-108
  34. Reed D. W., Clayton R. K., 1968, Isolation of a reaction center fraction from Rhodopseudomonas sphaeroides, Biochem. Biophs. Res. Commun., 30, 471-475
  35. Jose A., Cavaleiro S., Smith K. M., 1986, Chromatography of chlorophylls and bacteriochlorophylls, Talanta., 33, 963-971
  36. Lascelles J., 1978, Regulation of pyrrole synthesis, In: the photosynthetic bacteria, 1st ed., Clayton R. K., Sistrom W. R., Plenum Press, New York and London, 795-808pp
  37. Shiba T., Simidu U., Taga N., 1979, Another aerobic bacterium which contains bacteriochlorophyll a, Bull. Jpn. Soc. Sci. Fish., 45, 801
  38. Murtaugh M. A., Ma K. N., Benson J., Curtin K., Caan B., Slattery M. L., 2004, Antioxidants, carotenoids, and risk of rectal cancer, Am. J. Epidemiol., 159, 32-41
  39. Johnson E. A., 1991, Astaxanthin from microbial sources, Cri. Rev. Biotech., 11, 297-301
  40. Taga N., 1968, Some ecological aspects of marine bacteria in the Kuroshio current, Bull. Misaki Mar. Biol. Kyoto Univ., 12, 65-76
  41. Jeong Y. K., Choi B. D., Kang S. J., Jeong S. H., Lee Y. K., Kim H. Y., Jung M. J., 2000, Characteristic of carotenoid component from halophilic bacteria, Haloarcular sp. EH-1, Kor. J. Biotechnol. Bioeng., 15, 673-676
  42. Navarro J. C., Hontoria F., Varo I., Amat F., 1988, Effect of alternate feeding with a poor long-chain polyunsaturated fatty acid Artemia strain and a rich one for sea bass(Dicentrarchus labrax) and prawn (Penaeus Kerathurus) larvae, Aquaculture, 74, 307-317