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Isolation and Characteristics of Photosynthetic Bacterium, Erythrobacter longus SY-46 which Produces Bacterial Carotenoids

Bacterial Carotenoids를 생산하는 광합성세균 Erythrobacter longus SY-46의 분리 및 특성

  • Kim, Yun-Sook (Department of Microbiology, Pukyong National University) ;
  • Lee, Dae-Sung (Department of Microbiology, Pukyong National University) ;
  • Jeong, Seong-Yun (Korea Bio-IT Foundry Center, Pusan National University) ;
  • Lee, Won-Jae (Department of Microbiology, Pukyong National University)
  • 김윤숙 (부경대학교 미생물학과) ;
  • 이대성 (부경대학교 미생물학과) ;
  • 정성윤 (부산대학교 한국 Bio-IT 파운드리 센터) ;
  • 이원재 (부경대학교 미생물학과)
  • Published : 2008.04.30

Abstract

The aerobic photosynthetic bacterium, which produces bacterial carotenoids was isolated and identified from coastal marine environments. This bacterium was identified by 16S rDNA sequencing and designated as Erythrobacter longus SY-46. E. longus SY-46 was Gram negative and rod shape, and the optimal culture conditions were $25^{\circ}C$, pH 7.0, and 3.0% NaCl concentration, respectively. The carbon and nitrogen sources required for the optimal growth were lactose and tryptone, respectively. Fatty acid compositions of E. longus SY-46 were $C_{18:1}$(78.32%), v-linolenic acid($C_{18:3n9.12.15c}:3.83%$), margaric acid($C_{17:0}$: 3.38%), palmitic acid($C_{16:0}$: 3.07%), and docosahexaenoic acid($C_{22:6n3}$: 2.21%). In addition, E. longus SY-46 showed the characteristic absorption peaks of bacterial carotenoids(in the region of 450 to 480 nm) and bacteriochlorophyll(770 to 772 nm). Major carotenoids of E. longus SY-46 were polyhydroxylated xanthophylls such as fucoxanthin and zeaxanthin.

Keywords

Aerobic photosynthetic bacterium;Bacterial carotenoids;Bacteriochlorophyll;Erythrobacet longus SY-46

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