Isolation and Identification of Micromonospora sp. Showing Nematocidal Activity Against Pine Wood Nematode

소나무재선충에 대한 살선충 활성을 나타내는 Micromonospora sp.의 분리 및 동정

  • Park, Dong-Jin (Functional Metabolomics Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Jae-Chan (Functional Metabolomics Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Pan-Kyung (Functional Metabolomics Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Chang-Jin (Functional Metabolomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • 박동진 (한국생명공학연구원 기능대사물질연구센터) ;
  • 이재찬 (한국생명공학연구원 기능대사물질연구센터) ;
  • 김판경 (한국생명공학연구원 기능대사물질연구센터) ;
  • 김창진 (한국생명공학연구원 기능대사물질연구센터)
  • Published : 2008.03.31

Abstract

For the isolation of Actinomycetes showing nematocidal activity against Pine wood nematode, Bursaphelenchus xylophilus, about 2000 culture broth of Actinomycetes were tested and their activity were compared with that of Streptomyces avermitilis resulting a selected strain AW050027. The cultural, morphological and physiological analysis was performed for the identification of a selected strain. Phylogenetic analyses based on 165 rDNA gene sequences showed that the selected strain AW050027 belonged to the genus Micromonospora and M. corioriae $NAR01^T$ was the closest neighbors, sharing 98.9% 165 rDNA gene sequence similarity.

Keywords

Bursaphelenchus xylophilus;Micromonospora sp.;nematode;identification

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