Protoplast Formation of Collectotrichum acutum and the Assessment of Antifungal Activity of Several by using its Protoplasts

고추 탄저병군(Colletotrichum acutatum)의 원형질체 형성과 원형질체를 이용한 살균제 효과 검정

  • Kim, Myoung-Ki (Dept. of Plant Medicine, Chungbuk National University) ;
  • Kim, Heung-Tae (Dept. of Plant Medicine, Chungbuk National University)
  • 김명기 (충북대학교 농업생명환경대학 응용생명환경학부 식물의학) ;
  • 김흥태 (충북대학교 농업생명환경대학 응용생명환경학부 식물의학)
  • Published : 2008.03.31

Abstract

To obtain protoplasts of Colletotrichum acutatum JC24, conidia were inoculated onto cellophane membrane placed on PDA and incubated at $25^{\circ}C$ for 20 hrs under the dark condition. Cellophane membranes, where mycelia were incubated, were soaked into 2% lysing enzyme solution prepared with 0.02 M phosphate buffer (pH 7.0) including 1.2 M sorbitiol. After treatment in 2% enzyme solution for 2 - 3 hrs, it could be possible to harvest $2-3\;{\times}\;10^6$ protoplasts/mL. The effect of several fungicides on reversion ratio was determined by using the protoplasts obtained from C. acutatum JC24. Any protoplasts could not be reversed to mycelia on reversion PDA amended with $10\;{\mu}\;g\;mL^{-1}$ of propineb. With tebuconazole, inhibition ratio of protoplast reversion was 100 and 0.9% at 0.5 and $0.1\;{\mu}\;g\;mL^{-1}$, respectively, while inhibitory effect on mycelial growth was 85.1 and 75.7%. The inhibitory tendency of carbendazim on protoplast reversion was as same as mycelial growth. In the case of strobilurins, trifloxystrobin and kresoxim-methyl, they only could inhibit protoplast reversion of C. acutatum JC24, when salicylhydroxamic acid (SHAM) was amended into reversion PDA with strobilurins.

Keywords

Colletotrichum acutatum;protoplast;fungicidal activities;strobilurins;salicylhydroxamic acid

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