DOI QR코드

DOI QR Code

Determination of Optimum Rice Harvest Time by Change of the Glutelin Contents During the Maturity Period

벼 등숙기간 중 글루테린 함량 변화에 따른 쌀의 수확적기 판정

  • Shin, Pyung-Gyun (Mushroom Research Division, National Institute of Horticultural & Herval Science) ;
  • Chang, An-Cheol (Soil and Fertilizer Management Division, National Academy of Agricultural Science, RDA) ;
  • Hong, Seong-Chang (Soil and Fertilizer Management Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Ki-Sang (Soil and Fertilizer Management Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Keum-Hee (National Plant Quarantine Service, MAF) ;
  • Lee, Yong-Bok (Soil and Fertilizer Management Division, National Academy of Agricultural Science, RDA)
  • 신평균 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 장안철 (국립농업과학원 토양비료관리과) ;
  • 홍성창 (국립농업과학원 토양비료관리과) ;
  • 이기상 (국립농업과학원 토양비료관리과) ;
  • 이금희 (식물검역원 중부격리재배연구소) ;
  • 이용복 (국립농업과학원 토양비료관리과)
  • Published : 2008.12.31

Abstract

The change of glutelin contents in rice grain during the maturity period was investigated to determine optimum rice harvesting time. The glutelin content was increased with increasing time after heading. In this study, eight of glutelin subunits were found. Among the glutelin subunits, 7208-subunit (MW, 35 kD) contents was significantly increased at 65 days after heading compared with 55 and 60 days after heading. 7405-subunit (MW, 50 kD) contents was steadily increased with time after heading. The results showed that at 55th day after heading would be optimum time for harvest to get the low glutelin content of rice grain.

Keywords

Harvest time;proteomics;glutelin;glutelin subunits

References

  1. Shevchenko, A., Wilm, M., Vorm, O., and Mann. M. (1996) Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels, Anal. Chem. 68, 850-858 https://doi.org/10.1021/ac950914h
  2. Yamagata, H, Sugimoto, T., Tanaka K., and Kasai. Z. (1982) Biosynthesis of storage proteins in developing rice seeds, Plant Physiol. 70, 1094-1100 https://doi.org/10.1104/pp.70.4.1094
  3. Park, O. K. (2004) Proteomic studies in plants, J. Biochem. Molecular Biol. 37(1), 133-138 https://doi.org/10.5483/BMBRep.2004.37.1.133
  4. Kusuba, M., Miyahara, K., Iida, S., Fukuoka, H., Takano, T., Sassa, H., Nishimura, M., and Nishio. T. (2003) Low glutelin content1: A dominant mutation that suppresses the glutelin multigene family via RNA silencing in rice, The Plant Cell 15, 1455-1467 https://doi.org/10.1105/tpc.011452
  5. Nishimura, M., Kusaba, M., Miyahara, K., Nishio, T., Lida, S., Imbe, T., and Sato. H. (2005) New rice varieties with low levels of easy-to-digest protein, 'LGC-Katsu' and 'LGC-Jun', Breeding Science 55, 103-105 https://doi.org/10.1270/jsbbs.55.103
  6. Komatsu, S., Konish, H., Shen, S., and Yang. G. (2003) Rice proteomics. A step toward functional analysis of the rice genome, Mole Cell Proteomics 2.1, 2-10 https://doi.org/10.1074/mcp.R200008-MCP200
  7. Komatsu, S., Kojima, K., Suzuki, K., Ozaki, K., and Higo. K. (2004) Rice proteome database based on two-dementional poltacrylamide gel electrophoresis: its ststus in 2003, Nucleic Acids Research 32, D389-D392
  8. Koller, A., Washburn, M. P., Lange, B. M., Andon, N. L., Deciu, C., Haynes, P. A., Hays, L., Schieltz, D., Ulaszek, R., Wei, J., Wolters, D., and Yaster III. J. R. Y. (2002) Proteomic survey of metabolic pathways in rice. www.pnas.org/cgi/doi/10.1073/pnas. 172183199:1-6 https://doi.org/10.1073/pnas.022622699
  9. Tanaka, N., Mitsui, S., Hiroya, H., Yanagi, K., and Komatsu, S. (2005) Expression and function of proteins during development of the basal region in rice seedling, Mole Cell Proteomics 4.6, 796-808 https://doi.org/10.1074/mcp.M400211-MCP200
  10. Komatsu, S., Kajiwara, H., and Hirano. H. (1993) A rice protein library: a data-file of rice proteins separated by two-dementional electrophoresis, Theor. Appl. Genet. 86, 935-942
  11. Chae, J.C. (2002) Present status prospect of crop production technology to improve the crop quality and functionality, Korean J. Crop Sci. 47(S), 1-14
  12. Li, X., and Okita, T.W. (1993) Accumulation of prolamins and glutelins during rice seed development: a quantitative evaluation, Plant Cell Physiol. 34, 385
  13. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72, 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  14. Anderson, N.L., Esquer-Blasco, R., Hofmann, J.P., and Anderson, N.G., (1991) A two-dimensional gel database of rat liver proteins useful in gene regulation and drug effects studies, Electrophoresis 12(11), 907-30 https://doi.org/10.1002/elps.1150121110