DOI QR코드

DOI QR Code

Detection of estrogenic hormone 17β-estradiol in soil samples by a recombinant yeast bioassay and supercritical fluid extraction

  • Shim, Jae-Han ;
  • Kim, Mi-Ra ;
  • Topp, Edward ;
  • Choi, Jeong-Heui ;
  • Mamun, Iqbal Rouf
  • Published : 2008.12.31

Abstract

Recombinant yeast estrogenicity (YES) assay was used as a bioanalytical tool in order to screen $17{\beta}$-estradiol in the soil samples collected from different sites of South Korea. Solvent extraction and supercritical fluid extraction (SFE) methods were compared for the extraction of the estradiol from the soils. Most high detection of the estradiol based on YES assay was observed in the soils extracted with methanol. Different types of estrogenic hormones including $17{\beta}$-estradiol were suggested to be possibly exiting in the soils, since the methanol extracts of the soils showed an estrogenic activity that was not observed in the hexane extracts of the soil. SFE extracts showed estrogenic activity in some of the samples but methanol extract showed best activity.

Keywords

$17{\beta}$-Estradiol;Supercritical fluid extraction (SFE);Soil;Recombinant yeast estrogenicity (YES) assay

References

  1. Miller, W. R. and Sharpe, R. M. (1998) Environmental oestrogens and human reproductive cancers. Endocrinerelated Cancer 5, 69-96 https://doi.org/10.1677/erc.0.0050069
  2. Colucci, M. S., Bork, H. and Topp, E. (2001) Persistence of estrogenic hormones in agricultural solids: I. 17$\beta$-estradiol and estrone. J. Environ. Qual. 30, 2070-2076 https://doi.org/10.2134/jeq2001.2070
  3. Peterson, E. W., Davis, R. K. and Orndorff, H. A. (2000) 17$\beta$-estradiol as an indicator of animal waste contamination in mantled karst aquifers. J. Environ. Qual. 29, 826-834 https://doi.org/10.2134/jeq2000.00472425002900030019x
  4. Feldman, D., Tokes, L. G., Stathis P. A., Miller, S. C., Kurz, W. and Harvey, D. (1984) Identification of 17$\beta$ -estradiol as the estrogenic substance in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. 81, 4722-4726
  5. IUPAC Technical report on "Endocrine Disruptors in the Environment" (2003), Pure Appl. Chem., 75, 631-681 https://doi.org/10.1351/pac200375050631
  6. Colucci, M. S. and Topp, E. (2001) Persistence of estrogenic hormones in agricultural solids: II. 17$\alpha$ -Ethynylestradiol. J. Environ. Qual. 30, 2077-2080 https://doi.org/10.2134/jeq2001.2077
  7. Jobling, S., Nolan, M., Tyler, C. R., Brighty, G. and Sumpter, J. P. (1998) Widespread sexual disruption in wild fish Environ. Sci. Technol 32(17), 2498-2506 https://doi.org/10.1021/es9710870
  8. Aherne, G. W. and Briggs, R. (1989) The relevance of the presence of certain synthetic steroids in the aquatic environment. J. Pharm. Pharmacol. 41(10), 735-736 https://doi.org/10.1111/j.2042-7158.1989.tb06355.x
  9. Shore, L. S., Gurevitz, M. and Shemesh, M. (1993) Estrogen as an environmental pollutant. Bull. Environ. Contam. Toxicol. 51(3), 361-366
  10. Purdom, C. E., Hardiman, P. A., Bye, V. J., Eno, N. C., Tyler C. R. and Sumpter J. P. (1994) Estrogenic effects of effluents from sewage treatment works. Chem. Ecol. 8(4), 275-285 https://doi.org/10.1080/02757549408038554
  11. Desbrow, C., Routledge, E. J., Brighty, G. C., Sumpter, J. P. and Waldock, M. (1998) Identification of estrogenic chemicals in STW effluent. 1. Chemical fractionation and in vitro biological screening. Environ. Sci. Technol 32(11), 1549-1558 https://doi.org/10.1021/es9707973
  12. Lerch, O. and Zinn, P. (2003) Derivatisation and gas chromatography-chemical ionization mass spectrometry of selected synthetic and natural endocrine disruptive chemicals. J. Chromatogr. A. 991(1), 77-97 https://doi.org/10.1016/S0021-9673(03)00175-4
  13. Raman, D. R., Layton, A. C., Moody, L. B., Easter, L. P., Sayler, G. S., Burns, R. T. and Mullen, M. D. (2001) Degradation of estrogens in dairy waste solids: Effects of acidification and temperature. Transactions of the ASAE 44(6), 1881-1888
  14. Yu, Z., Xiao, B., Huang, W. and Peng, P. (2004) Sorption of steroid estrogens to soils and sediments. Environ. Toxicol. Chem. 23(3), 531-539 https://doi.org/10.1897/03-192
  15. Kim, S. D., Cho, J., Kim, I. S., Vanderford, B. J. and Snyder, S. A., (2007) Occurrence and removal of pharmaceuticals and endocrine disruptors in South Korean surface, drinking, and waste waters. Water Res. 41, 1013-1021 https://doi.org/10.1016/j.watres.2006.06.034
  16. Kawagoshi, Y., Fujita, Y., Kishi, I. and Fukunaga, I. (2003) Estrogenic chemicals and estrogenic activity in leachate from municipal waste landfill determined by yeast two-hybrid assay. J. Environ. Monit. 5, 269-274 https://doi.org/10.1039/b210962j
  17. Rehmann, K., Schramn, K. W. and Kettrup, A. A. (1999) Applicability of a yeast oestrogen screen-like activities in environmental samples. Chemosphere 34(14), 3303-3312
  18. Timms B. G., Howdeshell, K. L. Barton, L., Bradley, S. Richter, C. A. and. Saal F. S. (2005) Estrogenic chemicals in plastic and oral contraceptives disrupt development of the fetal mouse prostate and urethra. Proceedings of the National Academy of Sciences, USA 102(19), 7014-7019
  19. Routledge, E. J., and Sumpter, J. P. (1996) Estrogenic activity of surfactants and some of their degradation products assessed using a recombinant yeast screen Environ. Toxicol. Chem. 15(3), 241-248 https://doi.org/10.1897/1551-5028(1996)015<0241:EAOSAS>2.3.CO;2
  20. Topp, E. and Starratt, A. (2000) Rapid mineralization of the endocrine-disrupting chemical 4-nonylphenol in soil. Environ. Toxicol. Chem. 19(2), 313-318 https://doi.org/10.1897/1551-5028(2000)019<0313:RMOTED>2.3.CO;2
  21. King, J. W. (1998) Analytical supercritical fluid techniques and methodology: conceptualization and reduction to practice. J AOAC Internat. 81, 9-17
  22. King, J. W. (1989) Fundamentals and applications of supercritical fluid extraction in chromatographic science. J Chromatographic Sci. 27, 355-364 https://doi.org/10.1093/chromsci/27.7.355
  23. Belford, A. C., Horst, A. V., Vethaak, A. D., Schafer, A. J., Rijs, G. B. J., Wegener, J. and Cofino, W. P. (1999) Analysis and occurrence of estrogenic hormones and their glucuronides in surface water and waste water in The Netherlands. Sci. Total Environ. 225, 101-108 https://doi.org/10.1016/S0048-9697(98)00336-2
  24. Finlay-Moore, O., Hartel, P. G. and Cabrera, M. L. (2000) 17 beta-eatradiol and testosterone in soil and run-off from grasslands amended with broiler litter. J. Environ. Qual. 29(5), 1604-1611 https://doi.org/10.2134/jeq2000.00472425002900050030x
  25. Lee, L. S., Strock, T. J., Sarmah, A. K. and Rao, P. S. C. (2003) Sorption and dissipation of testosterone, estrogens, and their primary transformation products in soils and sediment. Environ. Sci. Technol 37(18), 4098-4105 https://doi.org/10.1021/es020998t
  26. Tashiro, Y., Takahira, K. and Nakanishi, Y. (2004) Improved application of recombinant yeast assays on environmental samples by size exclusion chromatography. J. Environ. Monit. 6, 546-551 https://doi.org/10.1039/b314116k
  27. Nishikawa, J., Saito, K., Goto, J., Dakeyama, F., Matsuo, M. and Nishihara, T. (1999) New screening methods for chemicals with hormonal activities using interaction of nuclear hormone receptor with coactivator. Toxicol. Appli. Pharmacol. 154, 76-83 https://doi.org/10.1006/taap.1998.8557
  28. Hanselman, T. A., Graetz, D. A., Wilkie, A. C., Szabo, N. J. and Diaz, C. S. (2006) Determination of Steroidal Estrogens in Flushed Dairy Manure Wastewater by Gas Chromatography-Mass Spectrometry. J Environ Qual 35, 695-700 https://doi.org/10.2134/jeq2005.0282