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Bacteriocidal Effects of Ultraviolet Irradiation for Reducing Bovine Mastitis Derived from Environmental Contamination

우분뇨 유래 젖소 유방염 저감을 위한 자외선 조사 살균의 효과 규명

  • Kim, Dong-Hyeok (College of Veterinary Medicine, Gyeongsang National University) ;
  • Lim, Jung-Ju (College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Jin-Ju (College of Veterinary Medicine, Gyeongsang National University) ;
  • Jang, Hong-Hee (College of Veterinary Medicine, Gyeongsang National University) ;
  • Jang, Dong-Il (Department of Bio-Industrial and Machinery, Chungnam National University) ;
  • Lee, Seung-Joo (Department of Bio-Industrial and Machinery, Chungnam National University) ;
  • Lee, Hu-Jang (College of Veterinary Medicine, Gyeongsang National University) ;
  • Min, Won-Gi (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kwon, Sun-Hong (Department of Bio-Industrial Machinery, Pusan National University) ;
  • Kim, Sang-Hun (College of Veterinary Medicine, Chungnam National University) ;
  • Oh, Kwon-Young (Department of Agricultural Engineering, National Academy of Agriculturral Science) ;
  • Kim, Suk (College of Veterinary Medicine, Gyeongsang National University)
  • 김동혁 (경상대학교 수의과대학) ;
  • 임정주 (경상대학교 수의과대학) ;
  • 이진주 (경상대학교 수의과대학) ;
  • 장홍희 (경상대학교 수의과대학) ;
  • 장동일 (충남대학교 농과대학) ;
  • 이승주 (충남대학교 농과대학) ;
  • 이후장 (경상대학교 수의과대학) ;
  • 민원기 (경상대학교 수의과대학) ;
  • 권순홍 (부산대학교 바이오산업기계공학과) ;
  • 김상훈 (충남대학교 수의과대학) ;
  • 오권영 (국립농업과학원 농업공학부) ;
  • 김석 (경상대학교 수의과대학)
  • Published : 2008.12.31

Abstract

Bovine mastitis is an important disease causing serious economic loss in dairy production and food poison in public health. The major causative agents of bovine mastitis include Escherichia coli (E. coli), Streptococcus agalactiae (S. agalactiae), Staphylococcus aureus (S. aureus). These bacteria were found in milk and environmental condition such as feces, water, soil and so on. Recently, many cases of mastitis are derived from environmental contamination of micro-organisms, which important factors for the spread of this disease in farm. Ultraviolet irradiation (UV) has been used as disinfection for waste and water in clinical and industrial facilities. Moreover the UV irradiation has been used as useful bactericidal agents to remove bacterial biofilms in environmental condition. In this study, we determined the bacterial replication in different percentage of water content (PWC) in sterilized saw dust and feces complexes from farm, and results showed that slightly decreased growth pattern of E. coli and S. agalactiae but increased growth pattern of S. aureus in various PWC (200, 400 and 600%) until 144 h incubation. In the bacteriocidal effect of UV irradiation to bacteria in saw dust and feces complex, the results showed that bacteriocidal effect was depended on the UV irradiation time, irradiation distance and PWC. Especially the antibacterial activity of UV irratiation is stronger in low PWC (50%), long time irradiation (50 sec), and short distance (5 cm) than other condition of this study. Furthermore UV irradiation with stirring showed increased the bactericidal effect compared without stirring. These results suggested that bovine mastitis causing agents may survive long time in environmental condition especially saw dust and feces complexes in farm and can cause a various disease including mastitis. Moreover, these data can be used as basis for application and development of UV disinfection to control of bovine mastitis from environmental contaminated bacteria in dairy farm.

Keywords

UV;bovine mastitis;environmental condition;disinfection;bacteriocidal agent

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