Characterization of Bacteria and Their Antibiotic Sensitivities in Porcine Liquid Semen

돼지 액상정액 내 세균오염과 항생제 감수성에 관한 연구

  • 유재원 (농촌진흥청 국립축산과학원) ;
  • 조규호 (농촌진흥청 국립축산과학원) ;
  • 홍준기 (농촌진흥청 국립축산과학원) ;
  • 김명직 (농촌진흥청 국립축산과학원) ;
  • 박준철 (농촌진흥청 국립축산과학원) ;
  • 정일병 (농촌진흥청 국립축산과학원) ;
  • 김인철 (농촌진흥청 국립축산과학원)
  • Published : 2008.12.31


The semen collection process in the porcine is far from being a sterile procedure. Consequently, porcine ejaculates commonly contain bacterial contaminants. The aim of this study is to identify the bacteria in porcine semen and to find the antibiotics resistance of bacteria. Twelve porcine originating from four AI center were used to collect semen. Bacteria were identified by automated instrument for rapid organism identification system and bacterial sensitivities of 8 antibiotics were tested. The Bacterial contaminants of Staphylococcus genus(37.8%), Proteus genus(7.0%), Bacillus genus (6.1%), Pasteulla genus(5.7%), Acinetobacte genus(5.2%), Serratia genus(4.3%) and others(33.9%) were frequently isolated. However, amikacin showed higher antibiotic sensitivity than other antibiotics. General sanitation protocols can contribute partly to inhibit the bacterial contamination, with monitoring boar housing, semen collection areas and the extended semen. But, proper selection of preservative antibiotics by microbial sensitivities can minimize the influence of bacteria.



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