Genetic Variation and Relationships of Korean Cattle(Hanwoo) and Foreign Breeds Using Microsatellite Markers

초위성체 유전표지를 이용한 한우와 외래품종간의 유전적 변이와 유연관계 분석

  • Oh, Jae-Don (Genomic Informatics Center, Hankyong National University) ;
  • Kong, Hong-Sik (Genomic Informatics Center, Hankyong National University) ;
  • Lee, Jae-Hyeon (Genomic Informatics Center, Hankyong National University) ;
  • Yang, Dae-Yong (Animal Products Grading Service) ;
  • Jeon, Gwang-Joo (Genomic Informatics Center, Hankyong National University) ;
  • Lee, Hak-Kyu (Genomic Informatics Center, Hankyong National University)
  • 오재돈 (한경대학교 유전정보연구소) ;
  • 공홍식 (한경대학교 유전정보연구소) ;
  • 이제현 (한경대학교 유전정보연구소) ;
  • 양대용 (축산물등급판정소) ;
  • 전광주 (한경대학교 유전정보연구소) ;
  • 이학교 (한경대학교 유전정보연구소)
  • Published : 2008.12.31


The purpose of this study was to assess the genetic variation and establish the relationship amongst Hanwoo, Angus and Holstein breed. The genetic characteristics and variability within Hanwoo(300), Angus(80) and Holstein(50) were estimated on the basis of relationships determined using the 10 kinds of microsatellite, which is located on different chromosomes. Frequencies of microsatellites markers were used to estimate heterozygosities, polymorphic information content(PIC) and genetic distances. The PICs ranged from 0.604 to 0.872(Hanwoo), from 0.562 to 0.812(Angus) and from 0.471 to 0.828(Holstein). Observed heterozygosity and PIC of Hanwoo are the highest among the analyzed breeds. Additionally, Estimates of genetic distance can be utilized to identify genetic relationships between Hanwoo and the other breed. Genetic distances(0.233) between Hanwoo and Angus was lower than distances between Hanwoo and Holstein(2.283). Also, Genetic distances between Angus and Holstein was shown for(2,400). The other side, each individuals were not ramified to different group and were spread evenly in phylogenetic dendrogram about all the populations.



  1. Ota, T. DISPAN. Pennsylvania State University. PA. USA. 1993
  2. Park, S. Microsatellite Toolkit For MS Excel 97 or 2000.(personnel communication). 2000
  3. Peelman, L, J., Mortiaux, F., Van Zeveren, A., Dansercoer, A., Mommens, G., Coopman, F., Bouquet, Y., Burny, A., Renaville, R. and Portetelle, D. 1998. Evaluation of the genetic variability of 23 bovine microsatellite markers in four Belgian cattle breeds. Anim Genet. Jun; 29(3):161-7
  4. Saitou, N. and Nei, M. The neighbor-joining method: A new method for reconstructing phylogenetic tree. Mol. Biol. Evol. 4:406-425. 1987
  5. Litt M, Luty J. A. A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am J Hum Genet. Mar; 44(3):397-401. 1989
  6. Luciana, P., Daniella, T., Andréa, P., Luiz, L. and Luciana, A. 2003. Genetic characterization of Aberdeen Angus cattle using molecular marker. Genetics and Molecular Biology. 26(2):133-137
  7. Bjornstad, G., Nilsen, N. O. and Roed, K. H. 2003. Genetic relationship between Mongolian and Norwegian horses. Anim. Genet. 34:55-58
  8. Hansen, C., Shrestha, J, N, B., Parker, R. J., Crow, G. H., McAlpine P. J. and Derr J. N. 2002. Genetic diversity among Canadienne, Brown Swiss, Holstein, and Jersey cattle of Canada based on 15 bovine microsatellite markers. Genome. 45:897-904
  9. Lee, C. and Pollak, E. J. 2002. Genetic antagonism between body weight and milk production in beef cattle. J. Anim. Sci. 80:316- 321
  10. Arranz, J. J., Bayon, Y. and San Primitivo, F. 1996. Comparison of protein markers and microsatellites in differentiation of cattle popu- lations. Anim. Genet. 27:415-419
  11. Barker, J. S. F., Tan, S. G., Selvaraj, O. S. and Mukherjee, T. K. 1997. Genetic variation within and relationships among populations of Asian water buffalo(Bubalus bubalis). Anim. Genet. 28: 1-13
  12. 임현태, 민희식, 문원곤, 이재봉, 김재환, 조인철, 이학교, 이용욱, 이정규, 전진태. 2005. 한우 생산이력제에 활용 가능한 Microsatellite의 분석과 선발. 동물자원과학회지. 47(4):491-500
  13. Blott, S. C., Williams, J. L. and Haley, C. S. 1999. Discriminating among cattle breeds using genetic markers. Heredity 82:613-619
  14. Li, K., Chen, Y., Moran, C., Fan, B., Zhao, S. and Peng, Z. 2000. Analysis of diversity and genetic relationships between four Chinese indigenous pig breeds and one Australian commercial pig breed. Anim. Genet. 31:322-325
  15. Martin-Burriel, I., Garcia-Muro, E. and Zaragoza, P. 1999. genetic diversity analysis of six Spanish native cattle breeds using microsatellites. Anim. Genet. 30:177-182
  16. Nei, M., Tajima, F. and Tateno, Y. 1983. Accuracy of estimated phylogenetic trees from molecular data. J. Mol. Evol. 19:153-170
  17. 윤두학, 박응우, 이승환, 이학교, 오성종, 정일정, 홍기창. 2005. Microsatellite loci 분석에 의한 한우와 타 품종간의 유전적 유연관계. 동물자원과학회지. 47(3):341-354

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