cDNA Microarray Analysis of Gene Expression in Pig Spleen Lymphocytes in Response to Extract of Raspberry

분자 추출물을 돼지의 비장 면역세포에 처리시 cDNA Microarray를 이용한 유전자 발현분석

  • Chung, Chung-Soo (Department of Animal Science, Chungbuk National University) ;
  • Choi, Young-Sook (Department of Animal Science, Chungbuk National University) ;
  • Lim, Hee-Kyong (Graduate School of Biotechnology & Information Technology, Hankyong National University) ;
  • O, Yun-Genel (Graduate School of Biotechnology & Information Technology, Hankyong National University) ;
  • Mandal, Prabhat Kumar (School of Biotechnology, Hankyong National University) ;
  • Choi, Kang-Duk (School of Biotechnology, Hankyong National University)
  • 정정수 (충북대학교 축산학과) ;
  • 최영숙 (충북대학교 축산학과) ;
  • 임희경 (한경대학교 생물환경, 정보통신전문대학원) ;
  • 오윤길 (한경대학교 생물환경, 정보통신전문대학원) ;
  • ;
  • 최강덕 (한경대학교 생명공학부)
  • Published : 2008.12.31


The present study was undertaken to investigate specific immune response of Rubus coreanus Miquel (raspberry) in pig spleen lymphocytes and gene expression induced by the extracts of raspberry using gene chip technology. The 70% ethyl alcohol extracts of raspberry were treated to pig spleen lymphocytes. The extracts of raspberry stimulated the proliferation of splenocytes and increased the population of CD3 & CD4 T-cells and B-cells in pig spleen lymphocytes. The extracts of raspberry improved immune response by increasing the viability of splenocytes. In microarray study we found eight genes were significantly up- regulated by the extracts of raspberry in pig splenocytes, including genes known to be involved in cell structure and immune response, particularly microtubule-associated protein 4, cytoplasmic dynein heavy chain, tumor necrosis factor alpha, lymphotoxin-beta receptor precursor. However, ten genes were down- regulated by the extracts of raspberry treatment.



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