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Analysis of Differential Expression of Cytochrome P450 Aromatase(Cyp19) in The Efferent Ductules and The Epididymis of Male Rats During Postnatal Development

생후 발달과정 동안 숫 흰쥐의 Efferent Ductules과 부정소에서 Cytochrome P450 Aromatase(Cyp19) 발현 양상 분석

  • Kim, Ju-Young (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine) ;
  • Seo, Hee-Jung (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine) ;
  • Kim, Ok-Soon (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine) ;
  • Kim, Byung-Joon (Departments of Biochemistry Internal Medicine, Eulji University School of Medicine) ;
  • Lee, Seong-Kyu (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine) ;
  • Baik, Haing-Woon (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine) ;
  • Lee, Ki-Ho (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine)
  • 김주영 (을지대학교 생화학-분자생물학교실 및 의과학연구소) ;
  • 서희정 (을지대학교 생화학-분자생물학교실 및 의과학연구소) ;
  • 김옥순 (을지대학교 생화학-분자생물학교실 및 의과학연구소) ;
  • 김병준 (을지대학교 생화학-내과학교실) ;
  • 이성규 (을지대학교 생화학-분자생물학교실 및 의과학연구소) ;
  • 백행운 (을지대학교 생화학-분자생물학교실 및 의과학연구소) ;
  • 이기호 (을지대학교 생화학-분자생물학교실 및 의과학연구소)
  • Published : 2008.12.31

Abstract

The present study was performed to determine expression of cytochrome P450 aromatase(Cyp19) in the efferent ductules(EDs) and the epididymis of male rat reproductive tract at different postnatal ages. Total RNAs isolated were reverse-transcribed, and cDNAs were utilized for real-time PCR analysis. In the EDs, the Cyp19 transcript was expressed at all prepubertal ages with the highest level at 14 days of age, but not at 90 days of age. Expression of Cyp19 mRNA in the epididymis was found at all age groups, except 7 days of age. Distinct expression patterns of Cyp19 transcript were shown in each segment of the epididymis. These results indicate that expression of Cyp19 gene in the excurrent duct of male reproductive tract is differentially regulated in age-dependent and segment-specific manners.

Keywords

References

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