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Expression Profiles of Triacylglycerol Biosynthesis Genes on Fattening Stages in Hanwoo

한우 비육기간 중 중성지방 생합성 관련 유전자의 발현양상

  • Kim, Nam-Kuk (National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Sung-Kon (National Institute of Animal Science, Rural Development Administration) ;
  • Heo, Kang-Nyeong (National Institute of Animal Science, Rural Development Administration) ;
  • Yoon, Duhak (National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Chang-Soo (Dept. of Applied Biochemistry, College of Biomedical & Health Science, Konkuk University) ;
  • Im, Seok-Ki (National Institute of Animal Science, Rural Development Administration) ;
  • Park, Eung-Woo (National Institute of Animal Science, Rural Development Administration)
  • 김남국 (농촌진흥청 축산과학원) ;
  • 김성곤 (농촌진흥청 축산과학원) ;
  • 허강녕 (농촌진흥청 축산과학원) ;
  • 윤두학 (농촌진흥청 축산과학원) ;
  • 이창수 (건국대학교 생명과학부 응용생화학전공) ;
  • 임석기 (농촌진흥청 축산과학원) ;
  • 박응우 (농촌진흥청 축산과학원)
  • Published : 2008.06.01

Abstract

Muscle fat content including intramuscular fat content(IMF) is the most important parameter in meat quality of cattle. Triacylglycerol is the major component of fat and is found in adipocyte and skeletal muscle. In present study, we carried out the determined of triacylglycerol contents and mRNA expression patterns of genes related with triacylglycerol biosynthesis such as ACSS2, GPAT, MGAT and DGAT in Hanwoo longissimus muscle using the real-time PCR. The triacylglycerol contents were continuously increased during the fattening stages. Interestingly, the contents of triacylglycerol were 7.4 fold higher(0.34 vs. 2.51, P<0.01) in 27 months old group than 12 months old group. The mRNA levels of ACSS2, GPAT and DGAT genes were also increased during fattening stages, whereas that of MGAT gene did not show difference among the stages. Thus, these results suggested that increasing of the triacylglycerol contents in longissimus muscle during fattening stages may be related with increased expressions of triacylglycerol biosynthesis genes(ACSS2, GPAT and DGAT). These results will be helpful to understand the mechanism of muscle fat deposition in skeletal muscle.

Keywords

Triacylglycerol; Real-time PCR; Fattening; Hanwoo

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