DOI QR코드

DOI QR Code

Isolation of a Bacillus licheniformis DK42 Producing Cellulase and Xylanase, and Properties of the Enzymes

Cellulase 및 Xylanase를 분비하는 Bacillus licheniformis DK42의 분리 및 효소 특성

  • Kim, Min Jeong (Dept. of Animal Resources Science, School of Bio-Resources Science, Dankook University) ;
  • Lim, Soo Jin (Dept. of Animal Resources Science, School of Bio-Resources Science, Dankook University) ;
  • Kang, Dae-Kyung (Dept. of Animal Resources Science, School of Bio-Resources Science, Dankook University)
  • 김민정 (단국대학교 생명자원과학대학 동물자원학과) ;
  • 임수진 (단국대학교 생명자원과학대학 동물자원학과) ;
  • 강대경 (단국대학교 생명자원과학대학 동물자원학과)
  • Published : 2008.06.01

Abstract

A bacterium producing cold-active cellulase and xylanase was isolated from pig feces. The isolate, DK42 strain, was found to be the Gram-positive, non-motile, catalase-positive, and spore-forming stain. Under an electron microscope, the cells were observed to be rod-shaped. The isolate was identified as Bacillus licheniformis DK42 on the basis of morphological and biochemical properties as well as 16S rRNA gene sequences. The characterization of crude cellulase and xylanase from B. licheniformis DK42 was investigated. Cellulase exhibited an optimum temperature and pH at 45℃ and 6.0, whereas xylanase exhibited an optimum temperature and pH at 55℃ and 6.0. Especially cellulase maintained approx. 50% of its maximum activity even at 10℃, indicating that it is cold-active. Both cellulase and xylanase were stable after 2hr at 35℃, whereas they lost their activities after 30min at 65℃.

Keywords

Cellulase; Xylanase; Bacillus licheniformis

References

  1. Akila, G. and Chandra, T. S. 2003. A novel cold-tolerant Clostridium strain PXYL1 isolated from a psychrophilic cattle manure digester that secretes themolabile xylanase and cellulase. FEMS Microbiol. Lett 219(1):63-67 https://doi.org/10.1016/S0378-1097(02)01196-5
  2. Campbell, G. L. and Bedford, M. R. 1992. Enzyme applications for monogastric feeds: A review. Can.. J. Anim. Sci. 72:449-453 https://doi.org/10.4141/cjas92-058
  3. Miller, G. L., Blum, R., Glennon, W. E. and Burton, A. L. 1960. Measurement of carboxy- methyl cellulase activity. Anal. Biochem. 2:127-132
  4. Omogbenigun, F. O., Nyachoti, C. M. and Slominski, B. A. 2004. Dietary supplementation with multienzyme preparations improves nutrient utilization and growth performance in weaned pigs. J. Anim. Sci. 82(4):1053-1061
  5. Sing, H. S., Madlala, A. M. and Prior, B. A. 2003. Thermomyces lanuginosus: properties of strains and their hemicellulases. FEMS Microbiol. Rev. 27:3-16 https://doi.org/10.1016/S0168-6445(03)00018-4
  6. Castanon, J. I. R., Flores, M. P. and Pettersson, D. 1997. Mode of degradation of non-starch polysaccharides by feed enzyme preparations. Anim. Feed Sci. Technol. 68:361-365 https://doi.org/10.1016/S0377-8401(97)00046-1
  7. Chiang, C. -C., Bi, Y. and Peter, W. -S. C. 2005. Effects of Xylanase supplementation to wheat-based diet on the performance and nutrient availability of Broiler Chickens. Asian-Aust. J. Anim. Sci. 18(8):1141-1146 https://doi.org/10.5713/ajas.2005.1141
  8. Kim, H., Park, J. S., Hancock, J. D., Hines, R. H., Cobb, C., Cao, H., Hong, J. W. and Kwon, O. S. 2003. Effects of amylase and cellulase supplementation in sorghum-based diets for finishing pigs. Asian-Aust. J. Anim. Sci. 16(11):70-76 https://doi.org/10.5713/ajas.2003.70
  9. 김창현, 이성실. 2003. 한우 및 산양의 장내 섬유소 분해 혐기 곰팡이의 분리 및 특성 규명. 한국동물자원학회지. 45(6):1007-1018
  10. 김태일, 한정대, 전병수, 하상우, 양창범, 김민균. 1999. 우분으로부터 Bacillus subtilis의 분리 및 균주가 분비하는 cellulase의 특성에 관한 연구. 한국미생물학회지. 35(4):277-282
  11. 정원형, 양시용, 송민동, 하종규, 김창원. 2003. Xylanase, cellulase의 생산성이 높은 Bacillus sp.의 분리 및 효소생산을 위한 배지조건의 최적화. 한국미생물생명공학회지. 31(4):383-388
  12. Sohn, J. H., Lee, J. H., Yi, H., Chun, J., Bae, K. S., Ahn, T. Y. and Kim, S. J. 2004. Kordia algicida gen. nov., sp. nov., an algicidal bacterium isolated from redtide. Int. J. Syst. Evol. Microbiol. 54:675-680 https://doi.org/10.1099/ijs.0.02689-0
  13. 김대준, 신한재, 민본홍, 윤기홍. 1995. 내열성 Cellulase-free Xylanase를 생산하는 고온성 Bacillus sp.의 분리 및 효소 특성. 산업미생물학회지. 23(3):304-310
  14. 김지연, 허성호. 홍정화. 2004. 알칼리성 Cellulase를 생산하는 호알칼리성 Bacillus sp. HSH-810의 분리 및 효소 특성. 한국미생물생명공학회지. 40 (2):139-146
  15. Beguin, P. and Aubert, J. P. 1994. The biological degradation of cellulase. FEMS Microbiol. Rev. 13:25-58 https://doi.org/10.1111/j.1574-6976.1994.tb00033.x
  16. Kiarie, E., Nyachoti, C. M., Slominski, B. A. and Blank, G. 2007. Growth performance, gastro- intestinal microbial activity, and nutrient digestibility in early-weaned pigs fed diets containing flaxseed and carbohydrase enzyme. J. Anim Sci. 85(11): 2982-2993 https://doi.org/10.2527/jas.2006-481
  17. Lee, J. -H. and Choi, S. H. 2006. Xylanase production by Bacillus sp. A-6 isolated from rice bran. J. Microbiol. Biotechnol. 16(12):1856-1861
  18. Veith, B., Herzberg, C., Steckel, S., Feesche, J., Maurer, K. H., Ehrenreich, P., Baumer, S., Henne, A., Liesegang, H., Merkl, R., Ehrenreich, A. and Gottschalk, G. 2004. The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential. J. Mol. Microbiol. Biotechnol. 7(4):204-211 https://doi.org/10.1159/000079829