Study on the Characteristics of DNA Comet Assay for Irradiated Vegetables and Grains

방사선조사된 채소류 및 곡물류의 DNA Comet Assay 특성 연구

  • Published : 2008.04.30


The possibility of DNA comet assay as a rapid method for screening the irradiated vegetables and grains was evaluated. Vegetables (spring onion, garlic, and tomato) irradiated at $0{\sim}3$ kGy and grains (rice flour and black soybean) irradiated at $0{\sim}9$ kGy were used as samples. Optimum DNA comet assay conditions, such as elution, sedimentation of suspension, and lysis time of cell, were established. The optimum conditions for vegetables were 10 min for the elution time, 0 min for the sedimentation time, and 5 min for the lysis time. The optimum conditions for grains were 15 min for the elution time, 60 min for the sedimentation time, and 30 min for the lysis time. For the food application of DNA comet assay, it was possible to screen various food samples irradiated at the following doses: spring onion at 2 kGy, garlic at 3 kGy, tomato at 1 kGy, rice flour at 9 kGy, and black soybean at 3 kGy. Each sample showed different forms and sizes in DNA comet. Therefore, further studies on various methods using comet shape, concentration, or area in DNA comet assay are necessary.


  1. Kwon JH. 2003. Commercialization of food irradiation technology and the identification of irradiated foods. Food Sci Ind 36: 50-55
  2. Oh KN, Kim KE, Yang JS. 2000. Detection of irradiated beans using the DNA comet assay. J Korean Soc Food Sci Nutr 29: 843-848
  3. Miyahara M, Saito A, Ito H, Toyoda M. 2000. Capability for identification of gamma-irradiated bovine liver by new high sensitivity comet assay. Biol Pharm Bull 23: 1399-1405
  4. Oh KN, Park JY, Kim KE, Yang JS. 2000. Detection of irradiated fruits using the DNA comet assay. Korean J Food Sci Technol 32: 531-537
  5. Delincee H. 2002. Analytical methods to identify irradiated food-a review. Radiat Phys Chem 63: 455-458
  6. Kim KS, Yang JS, Kwon JH. 2003. Method of detection for irradiated foods. Korean J Food Preserv 10: 427-434
  7. FAO/WHO CODEX STAN. 2003. General codex methods for the detection of irradiated foods. CODEX STAN 231-2001, Rev. 1
  8. Khan AA, Khan HM, Delincee H. 2002. Detection of radiation treatment of beans using DNA comet assay. Radiat Phys Chem 63: 407-410
  9. Kim CK, Yang JS, Lee HJ. 1999. Detection of irradiated grains using DNA comet assay. Korean J Food Sci Technol 31: 906-911
  10. Fairbairn DW, Olive PL, O'Neill KL. 1995. The comet assay: A comprehensive review. Mutat Res 339: 37-59
  11. Jeong SK, Park JH, Ji ST, Park KJ, Kim HH, Hyun CK, Shin HK. 2000. Discrimination of irradiated beef using comet assay. Korean J Food Sci Technol 32: 747-754
  12. An JH, Back MH, Kim JS, Jeong JH, Kwon ST. 2004. Assessment of nucleus-DNA damage in red pepper cells treated with γ-radiation through comet assay. Korean J Plant Biotechnol 31: 225-230
  13. Cerda H. 1998. Detection of irradiated fresh chicken, pork and fish using the DNA comet assay. Lebensm Wiss Technol 31: 89-92
  14. Lee EY, Yung JY, Jo DJ, Kwon JH. 2001. Detection characteristics of TL, ESR and DNA comet for irradiated peanuts by origins. J Korean Soc Food Sci Nutr 30: 1076-1081
  15. Cerda H, Delincee H, Haine H, Rupp H. 1997. The DNA 'comet assay' as a rapid screening technique to control irradiated food. Mutat Res 375: 167-181
  16. Byun MW, Lee JW. 2003. Application of irradiation technology for food safety and security. Food Sci Ind 36: 25-41