Antifungal Activity of Bacillus sp. AM-651 Against Phytophthora capsici

고추역병 유발병원균 Phytophthora capsici에 대한 Bacillus sp. AM-651의 항진균활성

  • Lee, Jung-Bok ;
  • Shin, Jeong-Hak (School of Bioresource Science, Andong National University) ;
  • Jang, Jong-Ok (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Shin, Kee-Sun (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Choi, Chung-Sik ;
  • Kim, Kun-Woo (School of Bioresource Science, Andong National University) ;
  • Jo, Min-Sub (School of Bioresource Science, Andong National University) ;
  • Jeon, Chun-Pyo (School of Bioresource Science, Andong National University) ;
  • Kim, Yun-Hoi (School of Bioresource Science, Andong National University) ;
  • Kwon, Gi-Seok (School of Bioresource Science, Andong National University)
  • 이중복 ((주)한스바이오) ;
  • 신정학 (안동대학교 생명자원과학부) ;
  • 장종옥 (한국생명공학연구원) ;
  • 신기선 (한국생명공학연구원) ;
  • 최충식 ((주)한스바이오) ;
  • 김건우 (안동대학교 생명자원과학부) ;
  • 조민섭 (안동대학교 생명자원과학부) ;
  • 전춘표 (안동대학교 생명자원과학부) ;
  • 김윤회 (안동대학교 생명자원과학부) ;
  • 권기석 (안동대학교 생명자원과학부)
  • Published : 2008.09.28

Abstract

Biological antagonists of Phytophthora capsici were isolated from soil in Gyeongbuk, Korea. Among the isolated bacteria, a Bacillus sp. was identified from l6S rDNA sequence analysis and named Bacillus sp. AM-651. Bacillus sp. AM-65l strain which can strongly a antifungal activity against Phytophthora capsici. Culture conditions for the maximum production of the antagonistic substance were optimized. The production of antibiotic were high on modified Davis mineral medium pH 7 at $30^{\circ}C$. The medium for highest production of the agonistic substance optimized. It is composed the best activity on glucose, $(NH_4)_2SO_4$ and $K_2HPO_4$ at 0.5%, 0.1%, and 0.7%, respectively. By time course of culture solution selected Bacillus sp. AM-65l, the culture solution after 48hrs had strongly growth inhibition rate against P. capsici. And culture solution of Bacillus sp. AM-651 was stable within a pH range $5{\sim}11$ and temperature range $4{\sim}70^{\circ}C$. Bacillus sp. AM-651 cultured broth shown fungal growth inhibitory activity against B. sorokiniana, B. cinerea, R. solani avove and beyond P. capsici and comparatively showed a high activity against C. gloeosporioides, B. dothidea, B. cinerea and F. graminearum by agar diffusion method.

Keywords

Antifungal;antibiotic;Bacillus sp. AM-651;Phytiphthora capsici;biopestcide

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