Antifungal Activity of Bacillus sp. AM-651 Against Phytophthora capsici

고추역병 유발병원균 Phytophthora capsici에 대한 Bacillus sp. AM-651의 항진균활성

  • Lee, Jung-Bok ;
  • Shin, Jeong-Hak (School of Bioresource Science, Andong National University) ;
  • Jang, Jong-Ok (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Shin, Kee-Sun (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Choi, Chung-Sik ;
  • Kim, Kun-Woo (School of Bioresource Science, Andong National University) ;
  • Jo, Min-Sub (School of Bioresource Science, Andong National University) ;
  • Jeon, Chun-Pyo (School of Bioresource Science, Andong National University) ;
  • Kim, Yun-Hoi (School of Bioresource Science, Andong National University) ;
  • Kwon, Gi-Seok (School of Bioresource Science, Andong National University)
  • 이중복 ((주)한스바이오) ;
  • 신정학 (안동대학교 생명자원과학부) ;
  • 장종옥 (한국생명공학연구원) ;
  • 신기선 (한국생명공학연구원) ;
  • 최충식 ((주)한스바이오) ;
  • 김건우 (안동대학교 생명자원과학부) ;
  • 조민섭 (안동대학교 생명자원과학부) ;
  • 전춘표 (안동대학교 생명자원과학부) ;
  • 김윤회 (안동대학교 생명자원과학부) ;
  • 권기석 (안동대학교 생명자원과학부)
  • Published : 2008.09.28


Biological antagonists of Phytophthora capsici were isolated from soil in Gyeongbuk, Korea. Among the isolated bacteria, a Bacillus sp. was identified from l6S rDNA sequence analysis and named Bacillus sp. AM-651. Bacillus sp. AM-65l strain which can strongly a antifungal activity against Phytophthora capsici. Culture conditions for the maximum production of the antagonistic substance were optimized. The production of antibiotic were high on modified Davis mineral medium pH 7 at $30^{\circ}C$. The medium for highest production of the agonistic substance optimized. It is composed the best activity on glucose, $(NH_4)_2SO_4$ and $K_2HPO_4$ at 0.5%, 0.1%, and 0.7%, respectively. By time course of culture solution selected Bacillus sp. AM-65l, the culture solution after 48hrs had strongly growth inhibition rate against P. capsici. And culture solution of Bacillus sp. AM-651 was stable within a pH range $5{\sim}11$ and temperature range $4{\sim}70^{\circ}C$. Bacillus sp. AM-651 cultured broth shown fungal growth inhibitory activity against B. sorokiniana, B. cinerea, R. solani avove and beyond P. capsici and comparatively showed a high activity against C. gloeosporioides, B. dothidea, B. cinerea and F. graminearum by agar diffusion method.


Antifungal;antibiotic;Bacillus sp. AM-651;Phytiphthora capsici;biopestcide


  1. Bowers, J. H. and Mitchell, D. J. 1991. Relationship between inoculum level of Phytophthora capsici and mortality of pepper. Phytopathol. 81: 178-184
  2. Chung, B. K. and M. J. Kang. 1990. Effect of temperature and nutrition affecting zoospore formation of Phytophthora capsici causing red pepper fruit rot. Kor. J. Mycol. 18: 203-208
  3. Kim, S. D. and H. S. Lim. 1990. The role of chitinase of Pseudomonas stutzeri YPL-1 in biocontrol of Fusarium solani. Kor. J. Microbiol. Biotechnol. 18: 88-94
  4. Kim, S. H., H. J. Suh, and C. O. Kim. 1993. Taxonomy, purification and physicochemical properties of novel antifungal antibiotic AF-011A. Kor. J. Appl. Microbiol. Biotechnol. 6: 556-536
  5. Lim, J. N. 2000. Phytophthora Diseases in korea. Plant Pathology Div. National Institute of Agricultultural Sicence and Technology, Rural Development Administration, Suwon 441-707. Korea
  6. Ristaino, J. B. 1990. Intraspecific variation among isolates of Phytophthora capsici from pepper and cucurbit fields in North in North Carolina. Phytopathol. 80: 1253-1259
  7. Kim, Y. S. and S. D. Kim. 1994. Antifungal mechanism and properties of antibiotic substances produced by Bacillus subtilis YB-70 as a biocontrol agent. J. Microbiol. Biotechnol. 4: 296-304
  8. Someya, N. and N. Kataoka. 2000. Biological control of cyclamen soilborne disease by Serratia marcescens strain B2. Plant disease. 4: 294-299
  9. Yoo, J. H., B. H. Song, J. G. Kim, M. H. Lee, and S. D. Kim. 1995. Genetic organization and nucleotide sequencing of the urea gene cluster in Bacillus pasteurii. Mol. cell. 5: 359-369
  10. Lamour, K. H. and M. K. Hausbeck. 2000. Mefenoxam insensitivity and the sexual stage of Phytophthora capsici in Michigan cucurbit fields. Phytopathol. 90: 369-400
  11. Lim, T. H., T. H. Chang, and B. J. Cha. 1998. Incidence of benzimidazole- and dicarboximide-resistant isolates of Monilinia fructicola from over-wintering memmies and peduncles on peach trees. Kor. J. Plant Pathol. 14: 367-370
  12. Lee, E. T. and S. D. Kim. 2001. An antifungal substance, 2,4-diacetylphloroglucinol, produced from antagonistic bacterium Pseudomonas fluorescens 2112 against Phytophthora capsici. Kor. J. Appl. Microbiol. Biotechnol. 29: 37-42
  13. Yoo, S. J. and H. G. Kim. 2002. Distribution and alteration of mating type of Phytophthora capsici population from red pepper in Korea. Kor. J. Mycol. 30: 152-156
  14. Paulitz, T. C. and J. E. Loper. 1991. Lack of a role for fluores cent siderphore production in the biological control of Phythium damping-off of cucumber by a strain of Pseudomonas putida. Phytopathol. 81: 930-935
  15. Oh, J. S. and C. H. Kim. 1992. Varying sensitivity to metalaxyl of Korean isolates of Phytophthora capsici from red pepper field. Kor. J. Plant Pathol. 8: 29-33
  16. Hwang, B. K. and B. S. Kim. 1995. In-vivo efficacy and invitro activity of tubercidine, and antibiotic nucleoside, for control of Phytophthora capsici blight in Capsicium annuum. Pestic. Sci. 44: 225-260
  17. Kim, Y. S. 1992. Biocontrol bacteria, Bacillus subtilis YB-70 producing the antifungal antibiotics and genetic improvement. Department of applied microbiology, Graduate school Yeungnam University
  18. Davidse, L. C., D. Looigen, L. J. Turkensteen, and D. Van der Wal. 1981. Occurrence of metalaxyl-resistant strains of Phytophthora infestans in Dutch potato field. Neth. J. Pl. Path. 87: 65-68
  19. Lim, H. S., J. M. Lee, and S. D. Kim. 2002. A plant growth promoting Pseudomonas fluorescens GL20 - mechanism for disease suppression, outer membrane receptors for ferric siderophore, and genetic improvement for increased biocontrol efficacy. J. Microbiol. Biochnol. 12: 240-249
  20. Shin, Y. J. 2000. Isolation, characteristics and structural analysis of the antifungal antibiotic from Bacillus sp. YJ-63. Department of applied microbiology, Graduate school Dongeui University
  21. Gregory, K. F., O. N. Allen, A. J. Riker, and W. H. Peterson. 1952. Antibiotics as agents for the control of certain damping-off fungi. Am. J. Botany. 9: 405-415
  22. Kim, K. D., S. Nemec, and G. Musson. 1997. Control of phytophthora root and crown rot of bell pepper with composts and soil amendments in the greenhouse. Apple. Soil Ecol. 5: 169-179