Expression of Human Heavy-Chain and Light-Chain Ferritins in Saccharomyces cerevisiae for Functional Foods and Feeds

Saccharomyces cerevisiae을 이용한 사람의 H-, L-ferritins 발현 연구

  • Han, Hye-Song (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Lee, Joong-Lim (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Park, Si-Hong (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kim, Jae-Hwan (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kim, Hae-Yeong (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University)
  • 한혜송 (경희대학교 생명자원과학연구원, 생명과학대학 식품생명공학과) ;
  • 이중림 (경희대학교 생명자원과학연구원, 생명과학대학 식품생명공학과) ;
  • 박시홍 (경희대학교 생명자원과학연구원, 생명과학대학 식품생명공학과) ;
  • 김재환 (경희대학교 생명자원과학연구원, 생명과학대학 식품생명공학과) ;
  • 김해영 (경희대학교 생명자원과학연구원, 생명과학대학 식품생명공학과)
  • Published : 2008.09.28

Abstract

To produce human ferritins in yeast, human H-chain and L-chain ferritins were amplified from previously cloned vectors. Each amplified ferritin gene was inserted into the pYES2.1/V5-His-TOPO yeast expression vector under the control of the GAL1promoter. Western blot analysis of the recombinant yeast cells revealed that H-and L-chain subunits of human ferritin were expressed in Saccharomyces cerevisiae. Atomic absorption spectrometry (AAS) analysis demonstrated that the intracellular content of iron in the ferritin transformant was 1.6 to 1.8-fold higher than that of the control strain. Ferritin transformants could potentially supply iron-fortified nutrients for food and feed.

Keywords

Atomic absorption spectrometry;human ferritin;iron;Saccharomyces cerevisiae

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