Characteristics of Rahnella aquatilis Strain AY2000 for an Anti-Yeast Substance Production

항효모성 물질 생산을 위한 Rahnella aquatilis AY2000 균주의 생육특성

  • Kang, Min-Jung (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Lee, Bok-Kyu (Department of Molecular Biology, Dong-Eui University) ;
  • Kim, Kwang-Hyeon (Department of Life Science and Biotechnology, Dong-Eui University)
  • 강민정 (동의대학교 자연과학대학 생명응용학과) ;
  • 이복규 (동의대학교 자연과학대학 분자생물학과) ;
  • 김광현 (동의대학교 자연과학대학 생명응용학과)
  • Published : 2008.09.28

Abstract

Rahnella aquatilis AY2000 has an unique characteristic which produces an anti-yeast substance (AYS). The AYS of the strain AY2000 was always secreted on agar plate, however, its activity in liquid culture was labile upon storage of the medium. In this paper, cultural conditions of the strain AY2000 for the AYS production were investigated in liquid culture, and minimal inhibitory concentration (MIC) against Saccharomyces cerevisiae was determined for the AYS activity. MIC of the AYS cultured in PYG broth at $^25{\circ}C$ for 24 hr was $23.5{\mu}g/mL$, however, that in MYCS (pH 5.5) broth at the same condition was $15.5{\mu}g/mL$. The activity of the AYS had increased rather in MYCS broth excluded $NH_4$-citrate than in the same broth contained $NH_4$-citrate, and MIC of the AYS produced in MYCS broth without $NH_4$-citrate was $15.5{\mu}g/mL$. When the strain AY2000 was maintained in MYCS broth without $NH_4$-citrate but added $100{\mu}M$ $FeCl_3$, the activity of the AYS had increased and its MIC was $7.8{\mu}g/mL$. MIC of the AYS was $7.8{\mu}g/mL$ after the strain AY2000 was cultured in MYCS broth containing $100{\mu}M$ $FeCl_3$ without $NH_4$-citrate, however, its MIC was $31.3{\mu}g/mL$ after 48-60 hr culture in the same broth.

Keywords

Anti-yeast substance;Saccharomyces cerevisiae;Rahnella aquatilis;growth inhibiton;MIC;liquid culture

References

  1. Cankar, K., H. Kraigher, M. Ravnikar, and M. Rupnik. 2005. Bacterial endophytes from seeds of Norwey spruce (Picea abies L. Karst). FEMS Microbiol. Lett. 244: 341-345 https://doi.org/10.1016/j.femsle.2005.02.008
  2. Carinder, J. E., J. D. Chua, R. B. Corales, A. J. Taege, and G. W. Procop. 2001. Rahnella aquatilis bacteremia in a patient with relapsed acute lymphoblastic leukemia. Scand. J. Infect. Dis. 33: 471-473 https://doi.org/10.1080/00365540152029972
  3. Evguenivieva-Hackenberg, E., and S. Selenska-Pobell. 1995. Genome analysis of five soil bacterial isolates named formally Enterobacter agglomerans. J. Appl. Bacteriol. 79: 49-60 https://doi.org/10.1111/j.1365-2672.1995.tb03123.x
  4. Gavini, F., C. Ferragut, B. Lefebvre, and H. Leclerc. 1976. Etude taxonomique d'enterobacteries appartenant ou apparentees au genre Enterobacter. Ann. Microbiol. (Paris) 127B: 317-335
  5. Niemi, R. M., M. P. Heikkila, K. Lahti, S. Kalso, and S. I. Niemela. 2001. Comparison of methods for determining the numbers and species distribution of coliform bacteria in well water samples. J. Appl. Microbiol. 90: 850-858 https://doi.org/10.1046/j.1365-2672.2001.01314.x
  6. Paludan-Muller, C., P. Dalgaard, H. H. Huss, and L. Gram. 1998. Evaluation of the role of Carnobacterium piscicola in spoilage of vacuum- and modified-atmosphere-packed cold-smoked salmon stored at $5^{\circ}C$. Int. J. Food Microbiol. 39: 155-166 https://doi.org/10.1016/S0168-1605(97)00133-5
  7. Kim, K. Y., D. Jordan, and H. B. Krishnan. 1997. Rahnella aquatilis, a bacterium isolated from soybean rhizosphere, can solubilize hydroxyapatite. FEMS Microbiol. Lett. 153: 273-277 https://doi.org/10.1016/S0378-1097(97)00246-2
  8. Hashidoko, Y., E. Itoh, K. Yokota, T. Yoshida, and S. Tahara. 2002. Characterization of five phyllosphere bacteria isolated from Rosa rugosa leaves, and their phenotypic and metabolic properties. Biosci. Biotechnol. Biochem. 66: 2474-2478 https://doi.org/10.1271/bbb.66.2474
  9. Lindow, S. E., C. Desurmont, R. Elkins, G. McGourty, E. Clark, and M. T. Brandl. 1998. Occurrence of indole-3-acetic acid-producing bacteria on pear trees and their association with fruit russet. Phytopathol. 88: 1149-1157 https://doi.org/10.1094/PHYTO.1998.88.11.1149
  10. Tallgren, A. H., U. Airaksinen, R. Weissenberg, H. Ojamo, J. Kuusisto, and M. Leisola. 1999. Exopolysaccharide-producing bacteria from sugar beets. Appl. Environ. Micriobiol. 65: 862-864
  11. Berge, O., T. Heulin, W. Achouak, C. Richard, R. Bally, and J. Balandreau. 1991. Rahnella aquatilis, a nitrogen-fixing enteric bacterium associated with the rhizosphere of wheat and maize. Can. J. Microbiol. 37: 195-203 https://doi.org/10.1139/m91-030
  12. National Committee for Clinical Laboratory Standards. 1995. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts: Tentative Standard M27-T. NCCLS, Vilanova, PA
  13. Bauman, P., D. J. Brenner, J. J. Farmer, W. Frederiksen, and J. M. Shewan. 1984. Facultatively anaerobic Gram-negative Rods, pp. 506-513. In N. R. Krieg and J. G. Holt (eds.), Bergey's Manual of Systematic Bacteriology, 9th Ed. Williams and Wilkins, Baltimore/London
  14. Heulin, T., O. Berge, P. Mavingui, L. Gouzou, K. P. Hebbar, and J. Balandreau, 1994. Bacillus polymyxa and Rahnella aquatilis, the dominant $N_{2}$-fixing bacteria associated with wheat rhizosphere in French soils. Eur. J. Soil Biol. 30: 35-42
  15. Iimura, K., and A. Hosono. 1996. Biochemical characteristics of Enterobacter agglomerans and related strains found in buck-wheat seeds. Int. J. Food Microbiol. 30: 243-253 https://doi.org/10.1016/0168-1605(96)00949-X
  16. Ryu. E. -J., H. -W. Kim, B.-W. KIM, H. -J. Kwon, and K.-H. Kim. 2006. Rahnella aquatilis strain AY2000 produces an anti-yeast substance. J. Microbiol. Biotechnol. 10: 1597-1604
  17. Hamze, M., J. Margaert, H. J. J. van Vuuren, F. Gavini, A. Beji, D. Izard, and K. Kersters. 1991. Rahnella aquatilis, a potential contaminant in lager beer breweries. Int. J. Food Microbiol. 13: 63-68 https://doi.org/10.1016/0168-1605(91)90137-E
  18. Brenner, D. J., H. E. Muller, A. G. Steigerwalt, A. M. Whitney, C. M. O'Hara, and P. Kalmpfer. 1998. Two new Rahnella genomospecies that cannot be phenotypically differentiated from Rahnella aquatilis. Int. J. Syst. Bacteriol. 48: 141-149 https://doi.org/10.1099/00207713-48-1-141
  19. El-Hendawy, H. H., M. E. Osman, and N. M. Sorour. 2005. Biological control of bacterial spot of tomato caused by Xanthomonas compestris pv. vescatoria by Rahnella aquatilis. Microbiol. Res. 160: 343-352 https://doi.org/10.1016/j.micres.2005.02.008
  20. Vasanthakumar, A., and P. S. McManus. 2004. Indole-3-acetic acid-producing bacteria are associated with cranberry stem gall. Phytopathol. 94: 1164-1171 https://doi.org/10.1094/PHYTO.2004.94.11.1164
  21. Laux, P., O. Baysal, and W. Zeller. 2002. Biological control of fire blight by using Rahnella aquatilis Ra39 and Pseudomonas spec. R1. Acta Hort. 590: 225-229
  22. Jensen, N., P. Varelis, and F. B. Whitfield. 2001. Formation of guaiacol in chocolate milk by the psychrotrophic bacterium Rahnella aquatilis. Lett. Appl. Microbiol. 33: 339-343 https://doi.org/10.1046/j.1472-765X.2001.01008.x
  23. Hamilton-Miller, J. M. T., and S. Shah, 2001. Identity and antibiotic susceptibility of enterobacterial flora of salad vegetables. Int. J. Antimicrob. Agents. 18: 81-83 https://doi.org/10.1016/S0924-8579(01)00353-3
  24. Lindberg, A.-M., A. Ljungh, S. Ahrne, G. Molin, and S. Lofdahl. 1998. Enterobacteriaceae found in high numbers in fish, minced meat and pasteurised milk or cream and presence of toxin encoding genes. Int. J. Food Microbiol. 39: 11-17 https://doi.org/10.1016/S0168-1605(97)00104-9