Characteristics of Rahnella aquatilis Strain AY2000 for an Anti-Yeast Substance Production

항효모성 물질 생산을 위한 Rahnella aquatilis AY2000 균주의 생육특성

  • Kang, Min-Jung (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Lee, Bok-Kyu (Department of Molecular Biology, Dong-Eui University) ;
  • Kim, Kwang-Hyeon (Department of Life Science and Biotechnology, Dong-Eui University)
  • 강민정 (동의대학교 자연과학대학 생명응용학과) ;
  • 이복규 (동의대학교 자연과학대학 분자생물학과) ;
  • 김광현 (동의대학교 자연과학대학 생명응용학과)
  • Published : 2008.09.28


Rahnella aquatilis AY2000 has an unique characteristic which produces an anti-yeast substance (AYS). The AYS of the strain AY2000 was always secreted on agar plate, however, its activity in liquid culture was labile upon storage of the medium. In this paper, cultural conditions of the strain AY2000 for the AYS production were investigated in liquid culture, and minimal inhibitory concentration (MIC) against Saccharomyces cerevisiae was determined for the AYS activity. MIC of the AYS cultured in PYG broth at $^25{\circ}C$ for 24 hr was $23.5{\mu}g/mL$, however, that in MYCS (pH 5.5) broth at the same condition was $15.5{\mu}g/mL$. The activity of the AYS had increased rather in MYCS broth excluded $NH_4$-citrate than in the same broth contained $NH_4$-citrate, and MIC of the AYS produced in MYCS broth without $NH_4$-citrate was $15.5{\mu}g/mL$. When the strain AY2000 was maintained in MYCS broth without $NH_4$-citrate but added $100{\mu}M$ $FeCl_3$, the activity of the AYS had increased and its MIC was $7.8{\mu}g/mL$. MIC of the AYS was $7.8{\mu}g/mL$ after the strain AY2000 was cultured in MYCS broth containing $100{\mu}M$ $FeCl_3$ without $NH_4$-citrate, however, its MIC was $31.3{\mu}g/mL$ after 48-60 hr culture in the same broth.


Anti-yeast substance;Saccharomyces cerevisiae;Rahnella aquatilis;growth inhibiton;MIC;liquid culture


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