Characterization of Viable But Nonculturable Condition of Escherichia coli Induced with Copper

구리에 의해 유도된 VBNC 대장균의 특성

  • Ku, Hyung-Keun (Health Metrology Center, Division of Metrology for Quality Life, Korea Research Institute of Standards and Science) ;
  • Park, Sang-Ryoul (Health Metrology Center, Division of Metrology for Quality Life, Korea Research Institute of Standards and Science) ;
  • Kim, Sook-Kyung (Health Metrology Center, Division of Metrology for Quality Life, Korea Research Institute of Standards and Science)
  • 구형근 (한국표준과학연구원, 삶의질 표준본부, 보건측정센터) ;
  • 박상열 (한국표준과학연구원, 삶의질 표준본부, 보건측정센터) ;
  • 김숙경 (한국표준과학연구원, 삶의질 표준본부, 보건측정센터)
  • Published : 2008.09.28

Abstract

VBNC (Viable but nonculturable) state is an adaptive response of cells in adverse environments, which lead cell not grow on routine nutrient agar. In this study, we induced VBNC in Escherichia coli using copper and verify the characterization of it. After treatment of copper, we didn't detect any cells via plate cultivation, namely, colony forming unit (CFU) was zero. However, we identified the existence of VBNC by staining live cells with Live/Dead BacLight bacterial viability kit and counting them through flow cytometry. Then we isolated genomic DNA and RNA from VBNC-induced cells and analyzed the stability of them. Degradation of RNA is more severe than that of DNA and RNA is degraded as specific fragments. In addition, we showed the morphology of VBNC cell by Bio-Transmission Electron Microscope (Bio-TEM). VBNC cell showed impaired periplasmic space and inner and outer membrane were separated and the amount of cytosol were significantly decreased.

Keywords

VBNC;Escherichia coli;copper;Bio-TEM

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