Evaluation of Antimicrobial, Antithrombin, and Antioxidant Activity of Aerial Part of Saxifraga stolonifera

호이초(Saxifraga stolonifera) 지상부의 항균, 항혈전 및 항산화 활성 평가

  • Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University) ;
  • Ryu, Hee-Young (Dept. of Food and Nutrition, Andong National University) ;
  • Jang, Yu-Jin (Agro-Biotechnology Education Center NURI, Kyungpook National University) ;
  • Jang, Han-Su (Gyeongbuk Institute for Bioindustry) ;
  • Park, Yu-Mi (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Kim, Sa-Youl (School of Life Science and Biotechnology, Kyungpook National University)
  • 손호용 (안동대학교 식품영양학과) ;
  • 류희영 (안동대학교 식품영양학과) ;
  • 장유진 (경북대학교 생물건강농업생명인재양성누리사업단) ;
  • 장한수 (경북바이오산업연구원) ;
  • 박유미 (경북대학교 생명공학부) ;
  • 김사열 (경북대학교 생명공학부)
  • Published : 2008.09.28

Abstract

Saxifraga stolonifera (Saxifragaceae) is a perennial herbaceous plant growing in Korea, China, Japan and Russia. The aerial part has been used as herbal medicine for treatment of pneumonia, frostbite, inflammation and microbial infection. In this study, fresh juice and methanol extract were prepared from the aerial part of S. stolonifera, and their antimicrobial, antithrombin, and antioxidant activity were evaluated, respectively. The fresh juice showed weak antimicrobial activity against Proteus vulgaris, Escherichia coli O157:H7 and Candida albicans with ignorable DPPH scavenging activity. But, the methanol extract showed strong antioxidant activity ($IC_{50}$ of $37.5{\mu}g/mL$) with minor, broad-range antimicrobial activity. Antithrombin activities were not observed in fresh juice and extract, up to 1.5 mg/mL. Sequential organic solvent fractionation of methanol extract showed that $IC_{50}s$ of ethylacetate and the butanol fraction were 6.9 and $7.8{\mu}g/mL$, respectively, that is comparable with vitamin C or butylated hydroxytoluene. Analysis of component in extract and fractionates suggested that the antioxidants in fractions are diverse and the active substances have glycosylated phenolic structure. Our results suggest that the aerial part of S. stolonifera could be used as the natural source of potential antioxidant.

Keywords

Antimicrobial;antioxidant;anti thrombosis;DPPH scavenging activity;Saxifraga stolonifera

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