Molecular Cloning of a Putative Gene Encoding Phospholipase B (plbA) from Aspergillus nidulans

사상설 진균 Aspergillus nidulans의 Phospholipase B 유전자(plb A)의 클로링

  • Hong, Sa-Hyun (Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korean National Institute of Health) ;
  • Cho, Eun-Min (Metabolome Analysis Team, Seoul Center, Korea Basic Science Institute) ;
  • Song, Seung-Eun (Metabolome Analysis Team, Seoul Center, Korea Basic Science Institute) ;
  • Eom, Chi-Yong (Metabolome Analysis Team, Seoul Center, Korea Basic Science Institute)
  • 홍사현 (국립보건연구원 감염병센터 장내세균팀) ;
  • 조은민 (한국기초과학지원연구원 서울센터 메타볼륨분석연구팀) ;
  • 송승은 (한국기초과학지원연구원 서울센터 메타볼륨분석연구팀) ;
  • 엄치용 (한국기초과학지원연구원 서울센터 메타볼륨분석연구팀)
  • Published : 2008.09.28

Abstract

The phospholipase B (PLB) families are enzymes sharing phospholipase (PL), lysophospholipase (LPL) and lysophospholipase-transacylase (LPTA) activities. In this study, we report the putative gene encoding phospholipase B (plbA) containing lipase motifs was cloned for the first time from the filamentous fungus, Aspergillus nidulans. plbA was isolated from A. nidulans genomic DNA library using a PCR-amplified probe, which is designed on the basis of sequence information derived from the conserved lipase regions of various PLBs. The deduced product of plbA is of 626 amino acids. From the assigned sequence, PlbA showed 72% identity with Penicillium notatum PLB but have low similarity with phospholipase A of other organisms.

Keywords

Aspergillus nidulans;fungi;Phospholipase B;PLB;plbA

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