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Optimization of the Medium Composition for Heteropolysaccharide-7 Production by Beijerinckia indica L3 Using Response Surface Methodology

표면반응방법을 이용한 Beijerinckia indica L3에 의한 PS-7 생산 최적화

  • Ra, Chae-Hun (Department of Biotechnology, Pukyong National University) ;
  • Kim, Ki-Myong (School of Life Sciences and Biotechnology, Korea University) ;
  • Hoe, Pil-Woo (Trade & Investment Promotion Team, Busan Metropolitan City) ;
  • Choi, Mi-Ran (Department of Biotechnology, Pukyong National University) ;
  • Kim, Sung-Koo (Department of Biotechnology, Pukyong National University)
  • Published : 2008.02.28

Abstract

The production of heteropolysaccharide-7 (PS-7) by Beijerinckia indica (B. indica L3) was evaluated in shaker flask culture. The medium optimization was studied using response surface methodology (RSM). A five-level three-factor central composite design was employed to determine the maximum PS-7 yield at optimum levels for whey lactose, glucose and ammonium nitrate contents. The validity of the model could be determined by the regression coefficient, $R^2$. The values of $R^2$ were 0.72, 0.64 and 0.85 in PS-7, DCW and viscosity, respectively. The optimal medium combinations of whey lactose, glucose and ammonium nitrate concentrations on the PS-7 production were whey lactose (2%), glucose (1 %) and ammonium nitrate 5 mM, respectively. The result indicated that PS-7 production was affected significantly by the addition of glucose to whey lactose based on medium and C/N ratio.

본 연구는 플라스크 배양을 통해 B. indica L3 균주를 가지고 PS-7을 생산할 수 있는 최적 조건을 조사하기 위해서 수행하였다. RSM을 통한 배지의 최적화 실험에서는 whey lactose, glucose, ammonium nitrate 세 가지의 독립변수의 조성에 따라 배양 72시간에 해당하는 DCW, PS-7, 그리고 점도의 변화에 대한 결과를 관찰하였다. 결정 계수(R square)의 값은 PS-7 생산량, DCW, 점성에서 각각 0.72, 0.64, 0.85로 신뢰성 있는 값을 획득하였다. Whey lactose와 glucose의 조합에 따른 PS-7 생산에 미치는 효과를 살펴보면 whey lactose (2%), glucose (1%)에서 최적의 PS-7 생산을 나타내었다. 결론적으로 glucose에 whey lactose를 기반으로 하는 배지 첨가와 C/N 비율이 PS-7의 생산에 큰 영향을 미침을 알 수 있었다.

References

  1. Cui, F. J., Y. Li, Z. H. Xu, H. Y. Xu, K. Sun and W. Y. Tao. 2005. Optimization of the medium composition for production of mycelial biomass and exo-polymer by Grifola frondosa GF9801 using response surface methodology. Bioresource Technology In Press
  2. Dlamini, A. M. and P. S. Peiris. 1997. Production of high viscosity whey broths by lactose utilizing Xanthomonas campestris strain. Appl. Environ. Microbiol. 50, 1483-1485
  3. Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers and F. Smith. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350-356 https://doi.org/10.1021/ac60111a017
  4. Fu, J. F. and Y. H. Tseng. 1990. Construction of lactose- utilizing Xanthomonas campestris and production of xanthan gum from whey. Appl. Environ. Microbiol. 56, 919-923
  5. Gillespie, T. and M. A. Hulme. 1969. On the single-point determination of intrinsic viscosity. J. Appl. Polym. Sci. 13, 2031 https://doi.org/10.1002/app.1969.070130921
  6. Jurgen, O. 2000. Respiratory protection of nitrogenase in Azotobacter species: is a widely held hypothesis unequivocally supported by experimental evidence: FEMS Microbiol. Rev. 24, 321-333 https://doi.org/10.1111/j.1574-6976.2000.tb00545.x
  7. Lee, J. H., J. H. Kim, I. H. Zhu, X. B. Zhan, J. W. Lee, D. H. Shin and S. K. Kim. 2001. Optimization of conditions for the production of pullulan and high molecular weight pullulan by Aureobasidium pullulans. Biotechnol. Lett. 23, 817-820 https://doi.org/10.1023/A:1010365706691
  8. Lee, J. H., J. H. Kim, M. R. Kim, S. M. Lim, S. W. Nam, J. W. Lee and S. K. Kim. 2002. Effect of dissolved oxygen concentration and pH on the mass production of high molecular weight pullulan by Aureobasidium pullulans. J. Microbiol. Biotechnol. 12, 1-7
  9. Mao, X. B., T. Eksriwong, S. Chauvatcharin, J. J. Zhong. 2005. Optimization of carbon source and carbon/nitrogen ratio for cordycepin production by submerged cultivation of medicinal mushroom Crodyceps militaris. Proc. Biochem. 40, 1667-1672 https://doi.org/10.1016/j.procbio.2004.06.046
  10. Stanford, P. A. and J. Baird. 1983. Industrial utilization of polysaccharide, In: polysaccharide II. Academic Press, London
  11. SAS Institute, SAS/STAT User's Guide, version 6, 4th eds., Cary, NC, 1990
  12. Sutherland, I. W. 1998. Novel and established applications of microbial polysaccharides. TIBTECH. 16, 41-46 https://doi.org/10.1016/S0167-7799(97)01139-6
  13. Schwartz, R. D. and E. Bodie. 1985. Production of high viscosity whey broths by lactose utilizing Xanthomonas campestris strain. Appl. Environ. Microbiol. 50, 1483-1485
  14. Vohra, A. and T. Satyanarayana. 2002. Statistical optimization of medium components by response surface methodology to enhance phytase production by Pichia anomala. Proc. Biochem. 37, 999-1004 https://doi.org/10.1016/S0032-9592(01)00308-9
  15. Wu, J. R., J. H. Son, K. M. Kim, S. W. Nam, J. W. Lee and S. K. Kim. 2005. Optimization of heteropolysaccharide-7 production by Beijerinckia indica. Kor. J. Microbiol. Biotechnol. 33, 117-122