Effect of Sodium Selenite and Zinc-L-selenomethionine on Performance and Selenium Concentrations in Eggs of Laying Hens

  • Chantiratikul, Anut (Faculty of Veterinary Medicine and Animal Science, Mahasarakham University Muang) ;
  • Chinrasri, Orawan (Faculty of Veterinary Medicine and Animal Science, Mahasarakham University Muang) ;
  • Chantiratikul, Piyanete (Department of Chemistry, Faculty of Science, Mahasarakham University)
  • Received : 2007.10.04
  • Accepted : 2008.03.04
  • Published : 2008.07.01


The objective of this study was to determine the effect of sodium selenite and zinc-L-selenomethionine on performance and egg Se concentration in laying hens. Two hundred and twenty-four CP Browns aged 71 weeks were divided according to a $2{\times}3$ factorial in a completely randomized design. One more group without additional Se supplementation was used as a negative control. Each treatment consisted of four replicates and each replicate contained eight laying hens. The dietary treatments were T1: basal diet; T2, T3 and T4: basal diets plus 0.3, 1.0 and 3.0 mg Se from sodium selenite/kg, respectively; T5, T6 and T7: basal diets plus 0.3, 1.0 and 3.0 mg Se from zinc-L-selenomethionine/kg, respectively. The findings revealed that feed conversion rate/kg eggs, egg production, egg weight, Haugh units and eggshell thickness were not affected by source and level of Se (p>0.05). Increasing level of dietary Se significantly increased (p<0.05 the Se content of eggs. Zinc-L-selenomethionine markedly increased p<0.05 egg Se concentration as compared with sodium selenite. The results indicated that Se source did not influence performance of laying hens. However, zinc-L-selenomethionine increased p<0.05 egg Se concentration more than sodium selenite.


Supported by : Masasarakham University


  1. Schrauzer, G. N. 2000. Selenomethionine: a review of its nutritional significance, metabolism and toxicity. J. Nutr. 130:1653-1656.
  2. Sheng, Z., L. Jiakui, L. Haijian, L. Bin and W. Xiaolong. 2002. Investigation of selenium content of egg samples from three major commercial cities along the lower reaches of the Changjiang River. Res. Vet. Sci. 72:7-9.
  3. Skrivan, M., J. Simane, G. Dlouha and J. Doucha. 2006. Effect of dietary sodium selenite, Se-enriched yeast and Se-enriched Chlorella on egg Se concentration, physical parameters on eggs and laying hens production. Czeck J. Anim. Sci. 51:163-167.
  4. Song, Z., Y. Guo and J. Yuan. 2006. Effects of dietary iodine and selenium on the activities of blood lymphocytes in laying hens. Asian-Aust. J. Anim. Sci. 19:713-719.
  5. Thomson, C. D. 1998. Selenium speciation in human body fluids. Analyst. 123:827-831.
  6. Utterback, P. L., C. M. Parson, I. Yoon and J. Butler. 2005. Effect of supplementing selenium yeast in diets of laying hens on egg selenium content. Poult. Sci. 84:1900-1901.
  7. Ward, T. 2003. Looking beyond selenium yeast. Feed Tech. 7(3):20-22.
  8. Choct, M., A. J. Naylor and N. Reinke. 2004. Selenium supplementation affects broiler growth performance, meat yield and feather coverage. Br. Poult. Sci. 45:677-683.
  9. Combs, G. F. and S. B. Combs. 1986. The Role of Selenium in Nutrition. Academic Press, Inc., New York.
  10. Geoge, B., S. Davis and T. L. Ward. 2004. A novel organic selenium source (zinc-L-selenomethionine, $Availa^{(R)}$ Se) for broilers. J. Anim. Sci. 82 (Suppl. 1):319.
  11. Girling, C. A. 1984. Selenium in agriculture and the environment. Agric. Eco. Eviron. 11:37-65.
  12. AOAC. 1999. Official Methods of Analysis. 16th ed. Association of Official Analysis Chemists, Washington, DC.
  13. Arthur, J. R. 1997. Non-glutathione peroxidase peroxidase functions of selenium. pp. 143-154. In: Biotechnogy in the Feed Industry (Ed. T. P. Lyons and K. A. Jacques). Redwood Books, Wiltshire, England.
  14. Ort, J. F. and J. D. Latshaw. 1978. The toxic level of sodium selenite in the diet of laying chickens. J. Nutr. 108:1114-1120.
  15. Pan, C., K. Huang, Y. Zhao, S. Qin. F. Chen and Q. Hu. 2007. Effect of selenium source and level in hen's diet on tissue selenium deposition and egg selenium concentration. J. Agric. Food Chem. 55:1027-1032.
  16. Paton, N. D., A. H. Cantor, A. J. Pescatore, M. J. Ford and C. A. Smith. 2002. The effect of dietary selenium source and level on the uptake of selenium by developing chick embryos. Poult. Sci. 81:1548-1554.
  17. Kim, Y. Y. and D. C. Mahan. 2003. Biological aspects of selenium in farm animals. Asian-Aust. J. Anim. Sci. 16:435-444.
  18. Lyons, M. P., T. T. Papazyan and P. F. Surai. 2007. Selenium in food chain and animal nutrition: lesson from nature-review. Asian-Aust. J. Anim. Sci. 20:1135-1155.
  19. National Research Council. 1994. Nutrition Requirement of Poultry, 9th ed. National Academy Press, Washington, DC. USA.
  20. Olivera, P., D. Backovic and S. Sladana. 2005. Dietary selenium supplementation of pigs and broilers as a way of producing selenium enriched meat. Acta Veterinaria (Beograd). 55:483-492.
  21. Golubkina, N. A. and T. T. Papazyan. 2006. Selenium distribution in eggs of avian species. Comp. Biochem. Physiol. B 145:384-388.
  22. Jiakui, L. and W. Xiaolong. 2004. Effect of dietary organic versus inorganic selenium in laying hens on the productivity, selenium distribution in egg and selenium content in blood, liver and kidney. J. Trace Elem. Med. Biol. 18:65-68.
  23. Joaquim, A. N., Y. Gelinas. A. Krushevska and R. M. Barnes. 1997. Determination of elements in biological and botanical materials by inductively coupled plasma atomic emission and mass spectrometry after extraction with a tertiary amine reagent. J. Anal. At. Spectrom. 12:1239-1242.
  24. Payne, R. L., T. K. Lavergne and L. L. Southern. 2005. Effect of inorganic versus organic selenium on hen production and egg selenium concentration. Poult. Sci. 84:232-237.
  25. Reasbeck, P. G., O. Gilbert, M. D. Barbezat, L. Fredrick, M. D. Weber, M. F. Robinson and C. D. Thomson. 1985. Selenium absorption by canine jejunum. Diges. Dis. Sci. 30:489-494.
  26. Richardson, S. M., P. D. Siciliano, T. E. Engle, C. K. Larson and T. L. Ward. 2006. Effect of selenium supplementation and source on the selenium status of horses. J. Anim. Sci. 84:1742-1748.
  27. SAS. 1996. $SAS/STAT^{(R)}$ User's Guide (Release 6.03 ed.). SAS Inst. Inc. Cary, NC.

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