Functional Equivalence of Translation Factor elF5B from Candida albicans and Saccharomyces cerevisiae

  • Jun, Kyung Ok (Department of Biological Sciences, Sunchon National University) ;
  • Yang, Eun Ji (Department of Food and Nutrition, Sunchon National University) ;
  • Lee, Byeong Jeong (Department of Biological Sciences, Sunchon National University) ;
  • Park, Jeong Ro (Department of Food and Nutrition, Sunchon National University) ;
  • Lee, Joon H. (Myung-Gok Eye Research Institute, Kim's Eye Hospital, Konyang University College of Medicine) ;
  • Choi, Sang Ki (Department of Biological Sciences, Sunchon National University)
  • 투고 : 2007.05.07
  • 심사 : 2007.12.03
  • 발행 : 2008.04.30


Eukaryotic translation initiation factor 5B (eIF5B) plays a role in recognition of the AUG codon in conjunction with translation factor eIF2, and promotes joining of the 60S ribosomal subunit. To see whether the eIF5B proteins of other organisms function in Saccharomyces cerevisiae, we cloned the corresponding genes from Oryza sativa, Arabidopsis thaliana, Aspergillus nidulans and Candida albican and expressed them under the control of the galactose-inducible GAL promoter in the $fun12{\Delta}$ strain of Saccharomyces cerevisiae. Expression of Candida albicans eIF5B complemented the slow-growth phenotype of the $fun12{\Delta}$ strain, but that of Aspergillus nidulance did not, despite the fact that its protein was expressed better than that of Candida albicans. The Arabidopsis thaliana protein was also not functional in Saccharomyces. These results reveal that the eIF5B in Candida albicans has a close functional relationship with that of Sacharomyces cerevisiae, as also shown by a phylogenetic analysis based on the amino acid sequences of the eIF5Bs.


Candida albicans;eIF5B;Evolution;FUN12;Phylogeny;Saccharomyces cerevisiae;Translation


연구 과제 주관 기관 : Korea Research Foundation


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